Construct: ORF ccsbBroadEn_04850
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012339.1_s300c1, BRDN0000387815
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP1R21 (129285)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 129285 | PPP1R21 | protein phosphatase 1 regul... | NM_001135629.3 | 100% | 100% | |
| 2 | human | 129285 | PPP1R21 | protein phosphatase 1 regul... | NM_152994.5 | 98.5% | 98.5% | 1935_1936ins33 |
| 3 | human | 129285 | PPP1R21 | protein phosphatase 1 regul... | NM_001193475.1 | 94.6% | 94.6% | 1599_1600ins93;1842_1843ins33 |
| 4 | human | 129285 | PPP1R21 | protein phosphatase 1 regul... | XM_017003301.1 | 78.8% | 78.8% | 0_1ins495 |
| 5 | human | 129285 | PPP1R21 | protein phosphatase 1 regul... | XM_017003302.1 | 77.4% | 77.4% | 0_1ins495;1440_1441ins33 |
| 6 | human | 129285 | PPP1R21 | protein phosphatase 1 regul... | XR_001738609.1 | 72.8% | 1_153del;1379_1451del;2567_3211del | |
| 7 | human | 129285 | PPP1R21 | protein phosphatase 1 regul... | NR_024188.3 | 72.8% | 1_188del;313_351del;2568_3212del | |
| 8 | human | 129285 | PPP1R21 | protein phosphatase 1 regul... | XR_001738608.1 | 71.3% | 1_153del;2120_2259del;2634_3278del | |
| 9 | mouse | 73825 | Ppp1r21 | protein phosphatase 1, regu... | NM_028658.4 | 86.7% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2409
- ORF length:
- 2340
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGCCTCGGCT GAGTTGCAGG GGAAGTACCA GAAGCTGGCT CAGGAGTACT 121 CGAAGCTTCG GGCTCAGAAT CAGGTTCTGA AAAAAGGTGT TGTGGATGAA CAAGCAAATT 181 CTGCAGCTTT AAAGGAGCAA CTGAAAATGA AGGATCAGTC ATTGAGAAAA CTACAACAGG 241 AAATGGACAG TTTGACATTT CGAAATCTGC AGCTTGCCAA GAGGGTAGAA CTACTTCAAG 301 ATGAACTAGC TCTAAGTGAA CCACGAGGCA AGAAAAACAA GAAAAGTGGA GAATCTTCTT 361 CTCAGTTGAG TCAAGAGCAG AAGAGTGTCT TTGATGAAGA TCTGCAAAAG AAGATAGAAG 421 AGAATGAACG GTTGCATATA CAATTTTTTG AAGCTGATGA GCAGCACAAG CATGTGGAAG 481 CAGAGCTGAG GAGTCGACTG GCCACTCTGG AGACAGAAGC AGCCCAGCAC CAAGCTGTGG 541 TTGACGGTCT CACCCGGAAG TACATGGAAA CCATTGAGAA GCTGCAGAAC GACAAGGCTA 601 AACTAGAAGT GAAATCTCAG ACTCTAGAAA AGGAAGCCAA GGAATGTCGA CTTCGAACGG 661 AAGAATGTCA ATTACAGTTA AAGACTCTTC ATGAAGATTT GTCAGGTAGA TTAGAGGAAT 721 CCTTATCAAT CATCAATGAA AAAGTACCTT TTAATGATAC AAAATATAGT CAGTACAACG 781 CTCTGAACGT TCCACTCCAC AATAGGAGAC ACCAGCTGAA GATGCGAGAT ATTGCTGGGC 841 AGGCCCTGGC TTTTGTTCAG GATCTTGTGA CGGCTCTTCT AAACTTTCAT ACCTACACAG 901 AACAGAGGAT TCAAATTTTT CCTGTTGATT CTGCCATTGA CACTATATCT CCATTGAATC 961 AGAAGTTCTC ACAATACCTT CATGAAAATG CGTCCTATGT CCGCCCTCTT GAGGAAGGAA 1021 TGCTTCATTT ATTTGAAAGT ATCACTGAGG ATACTGTGAC TGTCTTGGAG ACAACTGTGA 1081 AATTGAAAAC TTTTTCAGAA CACTTAACCT CCTACATATG TTTTCTTAGG AAGATTCTTC 1141 CCTATCAGTT AAAAAGTTTA GAAGAAGAAT GTGAATCCTC TCTTTGCACA TCTGCGTTAA 1201 GAGCCAGGAA TCTAGAGCTG TCCCAGGACA TGAAAAAAAT GACAGCTGTG TTTGAGAAGC 1261 TGCAGACTTA CATAGCTCTT CTTGCCTTGC CAAGTACAGA GCCAGATGGA CTCCTTCGGA 1321 CAAACTACAG TTCTGTGTTA ACAAATGTTG GTGCTGCTCT GCATGGATTT CATGACGTTA 1381 TGAAAGATAT TTCCAAACAT TATAGTCAAA AAGCTGCAAT AGAGCATGAA CTTCCAACAG 1441 CAACACAGAA GCTGATAACA ACTAATGACT GTATCCTGTC ATCAGTAGTG GCATTAACAA 1501 ATGGAGCAGG AAAGATTGCA TCCTTCTTCA GCAACAATTT GGACTACTTC ATTGCTTCAC 1561 TGAGCTATGG ACCTAAGGCA GCGAGTGGAT TCATTAGTCC TCTTTCAGCT GAATGCATGC 1621 TACAGTATAA GAAAAAAGCT GCTGCCTATA TGAAGTCTTT GAGAAAGCCC CTCTTGGAGT 1681 CTGTGCCTTA TGAAGAAGCA CTGGCAAACC GCCGCATCCT TCTCAGCTCT ACTGAAAGTC 1741 GAGAAGGCCT TGCACAGCAA GTTCAACAGA GTTTGGAAAA GATTTCTAAA CTGGAGCAGG 1801 AAAAAGAACA TTGGATGTTG GAAGCACAAT TAGCCAAAAT CAAGCTAGAG AAAGAAAACC 1861 AGCGAATTGC AGATAAGCTG AAGAATACAG GTAGTGCCCA GCTGGTTGGG CTGGCCCAGG 1921 AAAATGCTGC TGTGTCAAAT ACTGCTGGCC AGGATGAAGC CACAGCTAAG GCTGTGTTGG 1981 AGCCCATTCA GAGCACCAGT CTAATTGGGA CTTTAACCAG GACATCTGAC AGTGAGGTTC 2041 CAGATGTGGA ATCTCGTGAA GACTTAATTA AAAATCACTA CATGGCAAGG ATAGTGGAAC 2101 TTACGTCTCA GTTGCAGCTG GCTGACAGTA AGTCAGTGCA TTTTTATGCC GAGTGCCGAG 2161 CACTGTCTAA AAGACTGGCC TTGGCTGAAA AGTCTAAGGA AGCATTGACA GAAGAAATGA 2221 AACTTGCCAG TCAGAACATC AGCAGACTTC AGGATGAGCT GACAACTACC AAGAGGAGTT 2281 ACGAGGATCA GTTAAGTATG ATGAGTGACC ACCTGTGCAG CATGAATGAG ACATTATCTA 2341 AACAGAGAGA AGAGATTGAC ACACTAAAGA TGTCCAGTAA GGGGAATTCT AAAAAGAACA 2401 AGAGTCGATT GCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa 2461 tttgttgcaa cgaac