Transcript: Human NM_001193475.1

Homo sapiens protein phosphatase 1 regulatory subunit 21 (PPP1R21), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-03-03
Taxon:
Homo sapiens (human)
Gene:
PPP1R21 (129285)
Length:
3055
CDS:
186..2402

Additional Resources:

NCBI RefSeq record:
NM_001193475.1
NBCI Gene record:
PPP1R21 (129285)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001193475.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000161152 CGAACGGAAGAATGTCAATTA pLKO.1 771 CDS 100% 13.200 18.480 N PPP1R21 n/a
2 TRCN0000433993 GAACGGTTGCATATACAATTT pLKO_005 543 CDS 100% 13.200 18.480 N PPP1R21 n/a
3 TRCN0000159146 GCATGAATGAGACATTATCTA pLKO.1 2311 CDS 100% 5.625 7.875 N PPP1R21 n/a
4 TRCN0000162399 CGAGGATCAGTTAAGTATGAT pLKO.1 2273 CDS 100% 5.625 4.500 N PPP1R21 n/a
5 TRCN0000161022 GTGGATTCATTAGTCCTCTTT pLKO.1 1702 CDS 100% 4.950 3.960 N PPP1R21 n/a
6 TRCN0000415813 ATCTCGTGAAGACTTAATTAA pLKO_005 2042 CDS 100% 15.000 10.500 N PPP1R21 n/a
7 TRCN0000420625 AGATTCTTCCCTATCAGTTAA pLKO_005 1249 CDS 100% 13.200 9.240 N PPP1R21 n/a
8 TRCN0000424463 AGCTGAAGATGCGAGATATTG pLKO_005 931 CDS 100% 13.200 9.240 N PPP1R21 n/a
9 TRCN0000160578 CAGAAGCTGATAACAACTAAT pLKO.1 1563 CDS 100% 13.200 9.240 N PPP1R21 n/a
10 TRCN0000428030 TGAACGTTCCACTCCACAATA pLKO_005 901 CDS 100% 13.200 9.240 N PPP1R21 n/a
11 TRCN0000420304 TGACGTTATGAAAGATATTTC pLKO_005 1490 CDS 100% 13.200 9.240 N PPP1R21 n/a
12 TRCN0000160197 CGATTTGGTATCTATGGAATA pLKO.1 2750 3UTR 100% 10.800 7.560 N PPP1R21 n/a
13 TRCN0000419711 CTCTTTGCACATCTGCGTTAA pLKO_005 1297 CDS 100% 10.800 7.560 N PPP1R21 n/a
14 TRCN0000413901 GATGGACTCCTTCGGACAAAC pLKO_005 1422 CDS 100% 10.800 7.560 N PPP1R21 n/a
15 TRCN0000163353 GCCTTCAGGAAGCTAAAGTAT pLKO.1 2490 3UTR 100% 5.625 3.938 N PPP1R21 n/a
16 TRCN0000162313 CCATTGACACTATATCTCCAT pLKO.1 1051 CDS 100% 2.640 1.848 N PPP1R21 n/a
17 TRCN0000166013 GCTGACAGTAAGTCAGTGCAT pLKO.1 2112 CDS 100% 0.264 0.185 N PPP1R21 n/a
18 TRCN0000162521 CCTAGTAAACTAGTCAGTGTT pLKO.1 2518 3UTR 100% 0.000 0.000 N PPP1R21 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001193475.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04850 pDONR223 100% 94.6% 94.6% None 1599_1600ins93;1842_1843ins33 n/a
2 ccsbBroadEn_13142 pDONR223 100% 49.1% 49% None 1089_2214delinsG n/a
3 ccsbBroad304_13142 pLX_304 0% 49.1% 49% V5 1089_2214delinsG n/a
4 TRCN0000469788 TACCACTGTTAAGACACATGGTGA pLX_317 33.2% 49.1% 49% V5 1089_2214delinsG n/a
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