Construct: ORF ccsbBroadEn_05055
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007772.1_s300c1, BRDN0000396134
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAFA4 (151647)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151647 | TAFA4 | TAFA chemokine like family ... | NM_001005527.2 | 100% | 100% | |
| 2 | human | 151647 | TAFA4 | TAFA chemokine like family ... | NM_182522.5 | 100% | 100% | |
| 3 | human | 151647 | TAFA4 | TAFA chemokine like family ... | XM_011533371.1 | 77% | 72.5% | (many diffs) |
| 4 | human | 151647 | TAFA4 | TAFA chemokine like family ... | XM_011533372.1 | 77% | 72.5% | (many diffs) |
| 5 | mouse | 320701 | Fam19a4 | family with sequence simila... | NM_177233.5 | 85.2% | 90% | (many diffs) |
| 6 | mouse | 320701 | Fam19a4 | family with sequence simila... | XM_017321648.1 | 85.2% | 90% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 486
- ORF length:
- 420
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAG GTCCCCAAGG ATGAGAGTCT GTGCTAAGTC AGTGTTGCTG TCGCACTGGC 121 TCTTTCTAGC CTACGTGTTA ATGGTGTGCT GTAAGCTGAT GTCCGCCTCA AGCCAGCACC 181 TCCGGGGACA TGCAGGTCAC CACCAAATCA AGCAAGGGAC CTGTGAGGTG GTCGCCGTGC 241 ACAGGTGCTG CAATAAGAAC CGCATAGAAG AGCGGTCACA AACGGTCAAG TGCTCTTGCT 301 TCCCGGGACA GGTGGCGGGC ACAACTCGGG CTCAACCTTC TTGTGTTGAA GCTTCCATTG 361 TGATTCAGAA ATGGTGGTGT CACATGAATC CGTGTTTGGA AGGAGAGGAT TGTAAAGTGC 421 TGCCAGATTA CTCAGGTTGG TCCTGTAGCA GTGGCAATAA AGTCAAAACT ACGAAGGTAA 481 CGCGGTACCC AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc ttatcaattt 541 gttgcaacga ac