Construct: ORF ccsbBroadEn_05086
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014716.1_s300c1, BRDN0000394279
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRYGN (155051)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 155051 | CRYGN | crystallin gamma N | NM_144727.2 | 100% | 100% | |
| 2 | human | 155051 | CRYGN | crystallin gamma N | XM_005249952.4 | 80.6% | 76.5% | (many diffs) |
| 3 | human | 155051 | CRYGN | crystallin gamma N | NM_001308292.1 | 60.6% | 54.5% | (many diffs) |
| 4 | human | 155051 | CRYGN | crystallin gamma N | XM_017011773.1 | 60.6% | 54.5% | (many diffs) |
| 5 | human | 155051 | CRYGN | crystallin gamma N | XR_002956411.1 | 39.4% | (many diffs) | |
| 6 | human | 155051 | CRYGN | crystallin gamma N | XR_002956412.1 | 29% | (many diffs) | |
| 7 | human | 155051 | CRYGN | crystallin gamma N | NR_131779.1 | 22.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 615
- ORF length:
- 546
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACACAAAAG 61 TTGGCACCAT GGCGCAGCGC TCGGGGAAGA TCACTCTCTA TGAAGGCAAG CACTTCACAG 121 GGCAGAAGCT GGAGGTCTTC GGGGACTGTG ACAACTTCCA GGACCGGGGC TTTATGAACC 181 GAGTGAACTC CATCCACGTG GAGAGCGGAG CCTGGGTCTG CTTCAATCAC CCCGACTTCC 241 GGGGCCAGCA GTTCATCTTG GAGCACGGCG ACTACCCCGA CTTCTTCCGC TGGAACAGCC 301 ACAGTGACCA CATGGGCTCC TGTCGGCCTG TAGGAATGCA CGGAGAACAT TTCCGCCTAG 361 AAATCTTCGA GGGTTGCAAC TTCACGGGCC AGTGCCTGGA GTTCCTGGAG GACAGCCCCT 421 TCCTCCAGAG CAGGGGCTGG GTCAAGAACT GTGTGAACAC CATCAAGGTG TACGGGGACG 481 GAGCAGCATG GAGCCCTAGA AGCTTCGGAG CTGAGGACTT CCAGCTGAGC AGCTCTCTTC 541 AATCAGATCA AGGACCGGAA GAGGCCACCA CAAAACCAGC AACAACCCAG CCACCTTTCC 601 TGACTGCAAA CCTCTTGCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct 661 tatcaatttg ttgcaacgaa c