Transcript: Human NM_001308292.1

Homo sapiens crystallin gamma N (CRYGN), transcript variant 2, mRNA.

Source:
NCBI, updated 2018-07-01
Taxon:
Homo sapiens (human)
Gene:
CRYGN (155051)
Length:
2049
CDS:
254..631

Additional Resources:

NCBI RefSeq record:
NM_001308292.1
NBCI Gene record:
CRYGN (155051)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001308292.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000157301 CAATCAGATCAAGGACCGGAA pLKO.1 736 3UTR 100% 2.160 3.024 N CRYGN n/a
2 TRCN0000152640 GCTTTATGAACCGAGTGAACT pLKO.1 354 CDS 100% 4.950 3.465 N CRYGN n/a
3 TRCN0000158203 CTCTATGAAGGCAAGCACTTC pLKO.1 281 CDS 100% 4.050 2.835 N CRYGN n/a
4 TRCN0000156570 GAGCAGCTCTCTTCAATCAGA pLKO.1 723 3UTR 100% 3.000 2.100 N CRYGN n/a
5 TRCN0000153528 CTCTTCAATCAGATCAAGGAC pLKO.1 731 3UTR 100% 2.640 1.848 N CRYGN n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001308292.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05086 pDONR223 100% 60.6% 54.5% None (many diffs) n/a
2 ccsbBroad304_05086 pLX_304 0% 60.6% 54.5% V5 (many diffs) n/a
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