Construct: ORF ccsbBroadEn_05094
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014442.1_s300c1, BRDN0000387018
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRMT10B (158234)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | NM_144964.4 | 100% | 100% | |
| 2 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | XM_024447426.1 | 97.1% | 97.1% | 842_843ins27 |
| 3 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | XM_011517735.3 | 96% | 96% | 1_39del |
| 4 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | XM_011517736.3 | 93.3% | 93.3% | 1_39del;881_882ins27 |
| 5 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | NM_001286950.1 | 81% | 81% | 419_420ins153;689_690ins27 |
| 6 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | XM_017014313.2 | 80.3% | 71.3% | (many diffs) |
| 7 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | XM_005251375.2 | 75.3% | 75.3% | 185_186ins234 |
| 8 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | NM_001286952.1 | 72.4% | 72.4% | 185_186ins234;608_609ins27 |
| 9 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | XM_011517738.2 | 72.3% | 72.3% | 1_39del;224_225ins234 |
| 10 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | NM_001286951.1 | 69.8% | 68.9% | (many diffs) |
| 11 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | XM_011517739.3 | 69.6% | 69.6% | 1_39del;224_225ins234;647_648ins27 |
| 12 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | NM_001286953.2 | 62% | 50.7% | 0_1ins88;97_98ins245;509_510ins27 |
| 13 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | NM_001286954.2 | 62% | 44.1% | 0_1ins176;242_243ins157;509_510ins27 |
| 14 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | XM_005251379.2 | 58.2% | 58.2% | 0_1ins396 |
| 15 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | XM_011517742.1 | 48.2% | 42.3% | (many diffs) |
| 16 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | XM_011517743.2 | 48.2% | 42.3% | (many diffs) |
| 17 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | NR_104612.1 | 39.1% | 1_79del;500_611del;1140_2422del | |
| 18 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | XR_929185.2 | 24.3% | 1_105del;948_1033del;1140_3894del | |
| 19 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | XR_001746192.1 | 24.1% | 1_112del;533_644del;1173_3927del | |
| 20 | human | 158234 | TRMT10B | tRNA methyltransferase 10B | XR_001746191.2 | 13% | (many diffs) | |
| 21 | mouse | 69934 | Trmt10b | tRNA methyltransferase 10B | XM_006538245.3 | 67.7% | 67.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1014
- ORF length:
- 948
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGA CTGGAAATTG GAAGGGAGTA CTCAGAAAGT AGAGTCACCT GTGCTGCAGG 121 GGCAAGAAGG CATCCTAGAG GAGACAGGTG AAGATGGACT TCCTGAAGGC TTCCAGCTTC 181 TGCAGATCGA TGCGGAAGGC GAGTGCCAGG AGGGAGAGAT CCTGGCCACA GGCAGTACGG 241 CATGGTGCTC GAAAAATGTC CAGAGAAAAC AGAGACACTG GGAAAAGATA GTTGCAGCAA 301 AGAAGAGCAA AAGAAAGCAA GAAAAAGAAC GAAGAAAAGC CAATCGTGCA GAAAATCCAG 361 GCATTTGCCC CCAGCACAGC AAACGTTTCC TGAGAGCTCT AACCAAAGAC AAACTTTTGG 421 AAGCCAAACA CTCAGGACCA AGACTATGTA TCGATTTGAG TATGACCCAC TACATGTCAA 481 AGAAGGAATT AAGTAGACTG GCTGGACAGA TTCGAAGGTT GTATGGTTCA AACAAAAAAG 541 CTGACAGGCC ATTTTGGATC TGCCTCACTG GATTCACAAC AGACAGTCCC CTTTATGAAG 601 AGTGTGTGAG GATGAATGAT GGATTTTCTA GTTACCTGTT AGACATAACA GAAGAAGACT 661 GCTTTAGTTT ATTTCCCTTG GAAACCCTTG TGTACCTGAC TCCTGACTCA GAACACGCTC 721 TTGAAGATGT TGATCTAAAC AAAGTTTACA TCCTCGGTGG GCTTGTGGAT GAAAGCATTC 781 AGAAGAAGGT GACATTTCAA AAGGCCCGGG AATACTCTGT CAAGACCGCA CGCTTGCCAA 841 TCCAGGAATA CATGGTCAGA AACCAGAATG GGAAAAACTA TCATTCAGAG ATACTGGCCA 901 TCAATCAAGT GTTTGATATC CTGTCCACTT ACTTAGAGAC TCACAACTGG CCTGAAGCAT 961 TGAAGAAAGG AGTTTCTTCA GGAAAAGGCT ATATTCTTCG GAACTCAGTG GAATGCCCAA 1021 CTTTCTTGTA CAAAGTtggc attataagaa agcattgctt atcaatttgt tgcaacgaac