Transcript: Human NM_001286954.2

Homo sapiens tRNA methyltransferase 10B (TRMT10B), transcript variant 6, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
TRMT10B (158234)
Length:
2109
CDS:
254..844

Additional Resources:

NCBI RefSeq record:
NM_001286954.2
NBCI Gene record:
TRMT10B (158234)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001286954.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000034855 GCTCTTGAAGATGTTGATCTA pLKO.1 572 CDS 100% 4.950 3.960 N TRMT10B n/a
2 TRCN0000415525 GAAGACTGCTTTAGTTTATTT pLKO_005 509 CDS 100% 15.000 10.500 N TRMT10B n/a
3 TRCN0000413624 ACGCTTGCCAATCCAGGAATA pLKO_005 685 CDS 100% 10.800 7.560 N TRMT10B n/a
4 TRCN0000034857 GCTCTAACCAAAGACAAACTT pLKO.1 408 CDS 100% 5.625 3.938 N TRMT10B n/a
5 TRCN0000034856 CGATTTGAGTATGACCCACTA pLKO.1 464 CDS 100% 4.050 2.835 N TRMT10B n/a
6 TRCN0000034858 GTCAGAAACCAGAATGGGAAA pLKO.1 710 CDS 100% 4.050 2.835 N TRMT10B n/a
7 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 1391 3UTR 100% 4.950 2.475 Y ERAP2 n/a
8 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 1392 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001286954.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05094 pDONR223 100% 62% 44.1% None 0_1ins176;242_243ins157;509_510ins27 n/a
2 ccsbBroad304_05094 pLX_304 0% 62% 44.1% V5 0_1ins176;242_243ins157;509_510ins27 n/a
3 TRCN0000470516 ATCAGACTAACCGAGGATTAATAC pLX_317 47% 62% 44.1% V5 0_1ins176;242_243ins157;509_510ins27 n/a
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