Construct: ORF ccsbBroadEn_05691

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003894.1_s300c1, BRDN0000395813
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPIN2B (474343)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 474343 SPIN2B spindlin family member 2B NM_001006681.1 100% 100%
2 human 474343 SPIN2B spindlin family member 2B NM_001006682.2 100% 100%
3 human 474343 SPIN2B spindlin family member 2B NM_001006683.1 100% 100%
4 human 474343 SPIN2B spindlin family member 2B XM_005262009.5 100% 100%
5 human 474343 SPIN2B spindlin family member 2B XM_005262010.2 100% 100%
6 human 474343 SPIN2B spindlin family member 2B XM_011530788.3 100% 100%
7 human 474343 SPIN2B spindlin family member 2B XM_017029552.2 100% 100%
8 human 54466 SPIN2A spindlin family member 2A NM_019003.4 99.7% 99.6% 405C>T;700A>G
9 human 54466 SPIN2A spindlin family member 2A XM_005262016.4 99.7% 99.6% 405C>T;700A>G
10 human 54466 SPIN2A spindlin family member 2A XM_005262017.4 99.7% 99.6% 405C>T;700A>G
11 human 54466 SPIN2A spindlin family member 2A XM_005262019.3 99.7% 99.6% 405C>T;700A>G
12 human 54466 SPIN2A spindlin family member 2A XM_017029598.1 99.7% 99.6% 405C>T;700A>G
13 human 474343 SPIN2B spindlin family member 2B XM_005262008.5 84.8% 84.8% 1_138del
14 human 474343 SPIN2B spindlin family member 2B NM_001282461.2 60.8% 60.8% 222_223ins303
15 human 474343 SPIN2B spindlin family member 2B NM_001282462.2 60.8% 60.8% 222_223ins303
16 human 474343 SPIN2B spindlin family member 2B XM_011530789.2 60.8% 60.8% 222_223ins303
17 human 54466 SPIN2A spindlin family member 2A XM_005262018.5 60.7% 60.4% 222_223ins303;397A>G
18 mouse 278240 Spin2c spindlin family, member 2C NM_001005370.1 82.6% 81.8% (many diffs)
19 mouse 278240 Spin2c spindlin family, member 2C XM_006528868.3 82.6% 81.8% (many diffs)
20 mouse 278240 Spin2c spindlin family, member 2C XM_006528869.3 82.6% 81.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
840
ORF length:
774
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAA GACCCCCAAC GCACAGGAAG CCGAAGGGCA ACAAACCAGG GCAGCTGCAG
121GACGGGCCAC TGGGTCTGCA AACATGACAA AGAAAAAAGT CTCCCAAAAG AAGCAGAGAG
181GCCGACCTTC ATCCCAGCCC CGCAGGAACA TCGTGGGCTG CAGAATTTCT CATGGATGGA
241AGGAAGGAGA TGAGCCCATC ACGCAGTGGA AAGGAACCGT TCTGGATCAG GTGCCTATAA
301ATCCCTCTCT TTATCTGGTG AAATATGATG GAATTGACTG TGTCTATGGA CTGGAACTTC
361ACAGAGATGA AAGGGTTTTG TCTCTTAAAA TTCTTTCTGA CAGGGTGGCA TCATCTCACA
421TTAGTGATGC CAACCTTGCA AATACCATAA TTGGCAAAGC AGTGGAACAT ATGTTTGAGG
481GAGAGCATGG TTCTAAGGAT GAATGGAGGG GGATGGTCTT AGCTCAAGCA CCTATCATGA
541AAGCCTGGTT TTATATTACC TATGAGAAAG ATCCTGTCTT GTACATGTAC CAGCTTCTAG
601ATGATTATAA GGAAGGTGAC CTCCGCATCA TGCCAGAATC CAGTGAGTCT CCTCCAACAG
661AGAGGGAGCC AGGAGGAGTT GTAGATGGCC TAATAGGTAA GCATGTGGAA TATACCAAAG
721AAGATGGCTC CAAAAGGATC GGCATGGTCA TTCACCAAGT GGAAGCCAAA CCCTCTGTGT
781ATTTCATCAA GTTTGATGAT GATTTCCATA TCTATGTCTA CGATTTGGTG AAAAAGTCCT
841ACCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca
901acgaac

Download FASTA (ORF) (Full)