Construct: ORF ccsbBroadEn_05748
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010607.1_s300c1, BRDN0000393366
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CMC4 (100272147)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100272147 | CMC4 | C-X9-C motif containing 4 | NM_001018024.3 | 100% | 100% | |
| 2 | mouse | 105886298 | Cmc4 | C-x(9)-C motif containing 4 | NM_001294277.1 | 86.7% | 88.2% | (many diffs) |
| 3 | mouse | 105886298 | Cmc4 | C-x(9)-C motif containing 4 | NM_010839.6 | 86.7% | 88.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 270
- ORF length:
- 204
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCC GCAGAAGGAT CCGTGCCAGA AGCAAGCCTG TGAGATACAG AAATGTTTAC 121 AAGCCAACAG CTACATGGAA TCAAAGTGTC AGGCTGTCAT CCAAGAACTG CGTAAGTGTT 181 GTGCTCAGTA TCCCAAGGGA AGATCTGTCG TCTGTTCAGG ATTTGAAAAA GAAGAGGAAG 241 AAAACCTAAC ACGGAAGTCT GCATCAAAGT ACCCAACTTT CTGGTACAAA GTtggcatta 301 taagaaagca ttgcttatca atttgttgca acgaac