Construct: ORF ccsbBroadEn_05760
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003149.1_s300c1, BRDN0000388525
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACOX1 (51)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51 | ACOX1 | acyl-CoA oxidase 1 | NM_004035.7 | 99.8% | 99.6% | 458C>T;936C>G |
| 2 | human | 51 | ACOX1 | acyl-CoA oxidase 1 | NM_007292.6 | 95.3% | 95.1% | (many diffs) |
| 3 | human | 51 | ACOX1 | acyl-CoA oxidase 1 | NM_001185039.2 | 94.1% | 93.9% | 0_1ins114;344C>T;822C>G |
| 4 | human | 51 | ACOX1 | acyl-CoA oxidase 1 | XM_011524868.3 | 87.8% | 85.9% | (many diffs) |
| 5 | human | 51 | ACOX1 | acyl-CoA oxidase 1 | XM_011524869.3 | 79.2% | 78% | (many diffs) |
| 6 | mouse | 11430 | Acox1 | acyl-Coenzyme A oxidase 1, ... | NM_015729.3 | 83.8% | 87.7% | (many diffs) |
| 7 | mouse | 11430 | Acox1 | acyl-Coenzyme A oxidase 1, ... | NM_001271898.1 | 80.6% | 84.2% | (many diffs) |
| 8 | mouse | 11430 | Acox1 | acyl-Coenzyme A oxidase 1, ... | XM_006532002.3 | 74.7% | 74.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2046
- ORF length:
- 1980
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccCTTTTTG TACAAGAAAG 61 TTGGCATGAA CCCGGACCTG CGCAGGGAGC GGGATTCCGC CAGCTTCAAC CCGGAGCTGC 121 TTACACACAT CCTGGACGGC AGCCCCGAGA AAACCCGGCG CCGCCGAGAG ATCGAGAACA 181 TGATCCTGAA CGACCCAGAC TTCCAGCATG AGGACTTGAA CTTCCTCACT CGCAGCCAGC 241 GTTATGAGGT GGCTGTCAGG AAAAGTGCCA TCATGGTGAA GAAGATGAGG GAGTTTGGCA 301 TCGCTGACCC TGATGAAATT ATGTGGTTTA AAAATTTTGT GCACCGAGGG CGGCCTGAGC 361 CTCTGGATCT TCACTTGGGC ATGTTCCTGC CCACCTTGCT TCACCAGGCA ACTGCGGAGC 421 AGCAGGAGCG CTTCTTCATG CCCGCCTGGA ACTTGGAGAT CATTGGCACT TATGCCCAGA 481 CAGAGATGGG TCATGGAACT CACCTTCGAG GCTTGGAAAC CATAGCCACG TATGACCCTG 541 AAACCCAGGA GTTCATTCTC AACAGTCCTA CTGTGACCTC CATTAAATGG TGGCCTGGTG 601 GGCTTGGAAA GACTTCAAAT CATGCAATAG TTCTTGCCCA GCTCATCACT AAGGGGAAAT 661 GCTATGGATT ACATGCCTTT ATCGTACCTA TTCGTGAAAT CGGGACCCAT AAGCCTTTGC 721 CAGGAATTAC CGTTGGTGAC ATCGGCCCCA AATTTGGTTA TGATGAGATA GACAATGGCT 781 ACCTCAAAAT GGACAACCAT CGTATTCCCA GAGAAAACAT GCTGATGAAG TATGCCCAGG 841 TGAAGCCTGA TGGCACATAC GTGAAACCGC TGAGTAACAA GCTGACTTAC GGGACCATGG 901 TGTTTGTCAG GTCCTTCCTT GTGGGAGAAG CTGCTCGGGC TCTGTCTAAG GCGTGCACCA 961 TTGCCATCCG ATACAGCGCT GTGAGGCACC AGTCTGAAAT GAAGCCAGGT GAACCAGAAC 1021 CACAGATTTT GGATTTTCAA ACCCAGCAGT ATAAACTCTT TCCACTCCTG GCCACTGCCT 1081 ATGCCTTCCA GTTTGTGGGC GCATACATGA AGGAGACCTA TCACCGGATT AACGAAGGCA 1141 TTGGTCAAGG GGACCTGAGT GAACTGCCTG AGCTTCATGC CCTCACCGCT GGACTGAAGG 1201 CTTTCACCTC CTGGACTGCA AACACTGGCA TTGAAGCATG TCGGATGGCT TGTGGTGGGC 1261 ATGGCTATTC TCATTGCAGT GGTCTTCCAA ATATTTATGT CAATTTCACC CCAAGCTGTA 1321 CCTTTGAGGG AGAAAACACT GTCATGATGC TCCAGACGGC TAGGTTCCTG ATGAAAAGTT 1381 ATGATCAGGT GCACTCAGGA AAGTTGGTGT GTGGCATGGT GTCCTATTTG AACGACCTGC 1441 CCAGTCAGCG CATCCAGCCA CAGCAGGTAG CAGTCTGGCC AACCATGGTG GATATCAACA 1501 GCCCCGAAAG CCTAACCGAA GCATATAAAC TCCGTGCAGC CAGATTAGTA GAAATTGCTG 1561 CAAAAAACCT TCAAAAAGAA GTGATTCACA GAAAAAGCAA GGAGGTAGCT TGGAACCTAA 1621 CTTCTGTTGA CCTTGTTCGA GCAAGTGAGG CACATTGCCA CTATGTGGTA GTTAAGCTCT 1681 TTTCAGAAAA ACTCCTCAAA ATTCAAGATA AAGCCATTCA AGCTGTCTTA AGGAGTTTAT 1741 GTCTGCTGTA TTCTCTGTAT GGAATCAGTC AGAACGCGGG GGATTTCCTT CAGGGGAGCA 1801 TCATGACAGA GCCTCAGATT ACACAAGTAA ACCAGCGTGT AAAGGAGTTA CTCACTCTGA 1861 TTCGCTCAGA TGCTGTTGCT TTGGTTGATG CATTTGATTT TCAGGATGTG ACACTTGGCT 1921 CTGTGCTTGG CCGCTATGAT GGGAATGTGT ATGAAAACTT GTTTGAGTGG GCTAAGAACT 1981 CCCCACTGAA CAAAGCAGAG GTCCACGAAT CTTACAAGCA CCTGAAGTCA CTGCAGTCCA 2041 AGCTCTGCCC AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc ttatcaattt 2101 gttgcaacga ac