Construct: ORF ccsbBroadEn_05886

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000137.1_s300c1, BRDN0000396344
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BDNF (627)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 627 BDNF brain derived neurotrophic ... NM_001143805.1 99.7% 99.5% 196G>A;354T>C
2 human 627 BDNF brain derived neurotrophic ... NM_001143806.1 99.7% 99.5% 196G>A;354T>C
3 human 627 BDNF brain derived neurotrophic ... NM_001143807.2 99.7% 99.5% 196G>A;354T>C
4 human 627 BDNF brain derived neurotrophic ... NM_001143808.1 99.7% 99.5% 196G>A;354T>C
5 human 627 BDNF brain derived neurotrophic ... NM_001143811.1 99.7% 99.5% 196G>A;354T>C
6 human 627 BDNF brain derived neurotrophic ... NM_001143812.1 99.7% 99.5% 196G>A;354T>C
7 human 627 BDNF brain derived neurotrophic ... NM_001143813.2 99.7% 99.5% 196G>A;354T>C
8 human 627 BDNF brain derived neurotrophic ... NM_001143814.2 99.7% 99.5% 196G>A;354T>C
9 human 627 BDNF brain derived neurotrophic ... NM_001143816.1 99.7% 99.5% 196G>A;354T>C
10 human 627 BDNF brain derived neurotrophic ... NM_001709.5 99.7% 99.5% 196G>A;354T>C
11 human 627 BDNF brain derived neurotrophic ... NM_170732.4 99.7% 99.5% 196G>A;354T>C
12 human 627 BDNF brain derived neurotrophic ... NM_170733.3 99.7% 99.5% 196G>A;354T>C
13 human 627 BDNF brain derived neurotrophic ... NM_170735.5 99.7% 99.5% 196G>A;354T>C
14 human 627 BDNF brain derived neurotrophic ... NM_170731.5 96.6% 96.4% 1_24del;220G>A;378T>C
15 human 627 BDNF brain derived neurotrophic ... NM_170734.3 94% 93.8% 1_45del;241G>A;399T>C
16 human 627 BDNF brain derived neurotrophic ... NM_001143809.1 89.2% 89.1% 1_87del;283G>A;441T>C
17 human 627 BDNF brain derived neurotrophic ... NM_001143810.1 74.8% 74.7% 1_246del;442G>A;600T>C
18 human 627 BDNF brain derived neurotrophic ... XM_011520280.2 74.8% 74.7% 1_246del;442G>A;600T>C
19 mouse 12064 Bdnf brain derived neurotrophic ... NM_001048139.1 91.1% 96.3% (many diffs)
20 mouse 12064 Bdnf brain derived neurotrophic ... NM_001048141.1 91.1% 96.3% (many diffs)
21 mouse 12064 Bdnf brain derived neurotrophic ... NM_001048142.1 91.1% 96.3% (many diffs)
22 mouse 12064 Bdnf brain derived neurotrophic ... NM_001285416.1 91.1% 96.3% (many diffs)
23 mouse 12064 Bdnf brain derived neurotrophic ... NM_001285417.1 91.1% 96.3% (many diffs)
24 mouse 12064 Bdnf brain derived neurotrophic ... NM_001285418.1 91.1% 96.3% (many diffs)
25 mouse 12064 Bdnf brain derived neurotrophic ... NM_001285419.1 91.1% 96.3% (many diffs)
26 mouse 12064 Bdnf brain derived neurotrophic ... NM_001285420.1 91.1% 96.3% (many diffs)
27 mouse 12064 Bdnf brain derived neurotrophic ... NM_001285421.1 91.1% 96.3% (many diffs)
28 mouse 12064 Bdnf brain derived neurotrophic ... NM_001285422.1 91.1% 96.3% (many diffs)
29 mouse 12064 Bdnf brain derived neurotrophic ... NM_001316310.1 91.1% 96.3% (many diffs)
30 mouse 12064 Bdnf brain derived neurotrophic ... NM_007540.4 88.3% 93.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
807
ORF length:
741
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAC CATCCTTTTC CTTACTATGG TTATTTCATA CTTTGGTTGC ATGAAGGCTG
121CCCCCATGAA AGAAGCAAAC ATCCGAGGAC AAGGTGGCTT GGCCTACCCA GGTGTGCGGA
181CCCATGGGAC TCTGGAGAGC GTGAATGGGC CCAAGGCAGG TTCAAGAGGC TTGACATCAT
241TGGCTGACAC TTTCGAACAC ATGATAGAAG AGCTGTTGGA TGAGGACCAG AAAGTTCGGC
301CCAATGAAGA AAACAATAAG GACGCAGACT TGTACACGTC CAGGGTGATG CTCAGTAGTC
361AAGTGCCTTT GGAGCCTCCT CTTCTCTTTC TGCTGGAGGA ATACAAAAAT TACCTAGACG
421CTGCAAACAT GTCCATGAGG GTCCGGCGCC ACTCTGACCC TGCCCGCCGA GGGGAGCTGA
481GCGTGTGTGA CAGTATTAGT GAGTGGGTAA CGGCGGCAGA CAAAAAGACT GCAGTGGACA
541TGTCGGGCGG GACGGTCACA GTCCTTGAAA AGGTCCCTGT ATCAAAAGGC CAACTGAAGC
601AATACTTCTA CGAGACCAAG TGCAATCCCA TGGGTTACAC AAAAGAAGGC TGCAGGGGCA
661TAGACAAAAG GCATTGGAAC TCCCAGTGCC GAACTACCCA GTCGTACGTG CGGGCCCTTA
721CCATGGATAG CAAAAAGAGA ATTGGCTGGC GATTCATAAG GATAGACACT TCTTGTGTAT
781GTACATTGAC CATTAAAAGG GGAAGATACC CAACTTTCTT GTACAAAGTt ggcattataa
841gaaagcattg cttatcaatt tgttgcaacg aac

Download FASTA (ORF) (Full)