Construct: ORF ccsbBroadEn_05973

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008890.1_s300c1, BRDN0000396885
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CDC27 (996)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 996 CDC27 cell division cycle 27 NM_001114091.4 99.9% 99.8% 1225A>G
2 human 996 CDC27 cell division cycle 27 XM_011525546.3 99.8% 99.7% 1225A>G;1394_1395insAGA
3 human 996 CDC27 cell division cycle 27 NM_001256.6 99.2% 99.1% 955_956insAGACTTTTCGTGTTTTAC;1207A>G
4 human 996 CDC27 cell division cycle 27 NM_001293089.3 99.1% 99% 955_956insAGACTTTTCGTGTTTTAC;1207A>G;1376_1377insAGA
5 human 996 CDC27 cell division cycle 27 XM_011525547.3 93.3% 90.6% 1225A>G;2251_2252ins64;2325_2326ins101
6 human 996 CDC27 cell division cycle 27 XM_017025481.2 93.2% 90.4% (many diffs)
7 human 996 CDC27 cell division cycle 27 NM_001353035.2 92.6% 89.8% (many diffs)
8 human 996 CDC27 cell division cycle 27 XM_011525548.3 92.6% 89.6% 0_1ins35;68_69ins148;1042A>G
9 human 996 CDC27 cell division cycle 27 XM_011525549.2 92.6% 89.6% 0_1ins35;68_69ins148;1042A>G
10 human 996 CDC27 cell division cycle 27 NM_001353047.2 92.4% 89.7% (many diffs)
11 human 996 CDC27 cell division cycle 27 NM_001293091.3 91.8% 88.9% (many diffs)
12 human 996 CDC27 cell division cycle 27 XM_024451052.1 91.8% 88.9% (many diffs)
13 human 996 CDC27 cell division cycle 27 XM_017025484.2 91.7% 88.7% (many diffs)
14 human 996 CDC27 cell division cycle 27 NM_001353050.2 87.5% 84.4% 0_1ins35;67_68ins274;916A>G
15 human 996 CDC27 cell division cycle 27 XM_017025485.2 86.8% 83.7% (many diffs)
16 human 996 CDC27 cell division cycle 27 XM_017025486.1 86.7% 83.6% (many diffs)
17 human 996 CDC27 cell division cycle 27 NM_001353049.2 85.2% 79.7% (many diffs)
18 human 102723570 CDC27P9 cell division cycle 27 pseu... XR_430342.4 75.2% (many diffs)
19 human 102724621 LOC102724621 cell division cycle protein... XR_002958855.1 75.2% (many diffs)
20 human 102723584 CDC27P10 cell division cycle 27 pseu... XR_430343.4 74.3% (many diffs)
21 human 102723603 CDC27P11 cell division cycle 27 pseu... XR_430344.3 54.6% (many diffs)
22 human 996 CDC27 cell division cycle 27 XM_017025488.1 51.4% 51.3% 0_1ins1209;16A>G
23 human 996 CDC27 cell division cycle 27 NM_001353051.2 51.2% 51.2% 0_1ins1209;16A>G;185_186insAGA
24 human 996 CDC27 cell division cycle 27 XR_934630.2 44.3% (many diffs)
25 human 996 CDC27 cell division cycle 27 NR_148340.2 37.7% (many diffs)
26 mouse 217232 Cdc27 cell division cycle 27 XM_006533050.2 92.7% 96.7% (many diffs)
27 mouse 217232 Cdc27 cell division cycle 27 NM_001285988.1 92.6% 96.6% (many diffs)
28 mouse 217232 Cdc27 cell division cycle 27 NM_145436.2 92% 96.1% (many diffs)
29 mouse 217232 Cdc27 cell division cycle 27 NM_001285989.1 91.9% 96% (many diffs)
30 mouse 217232 Cdc27 cell division cycle 27 NM_001285990.1 77.2% 78.7% (many diffs)
31 mouse 217232 Cdc27 cell division cycle 27 XM_006533051.2 55.7% 59% (many diffs)
32 mouse 217232 Cdc27 cell division cycle 27 XM_006533052.3 55.7% 59% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2556
ORF length:
2490
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAC GGTGCTGCAG GAACCCGTCC AGGCTGCTAT ATGGCAAGCA CTAAACCACT
121ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA TGCAGAAGTA CACTCAGAAG
181AAGCCTTGTT TTTACTGGCA ACCTGTTATT ACCGCTCAGG AAAGGCATAT AAAGCATATA
241GACTCTTGAA AGGACACAGT TGTACTACAC CGCAATGCAA ATACCTGCTT GCAAAATGTT
301GTGTTGATCT CAGCAAGCTT GCAGAAGGGG AACAAATCTT ATCTGGTGGA GTGTTTAATA
361AGCAGAAAAG CCATGATGAT ATTGTTACTG AGTTTGGTGA TTCAGCTTGC TTTACTCTTT
421CATTGTTGGG ACATGTATAT TGCAAGACAG ATCGGCTTGC CAAAGGATCA GAATGTTACC
481AAAAGAGCCT TAGTTTAAAT CCTTTCCTCT GGTCTCCCTT TGAATCATTA TGTGAAATAG
541GTGAAAAGCC AGATCCTGAC CAAACATTTA AATTCACATC TTTACAGAAC TTTAGCAACT
601GTCTGCCCAA CTCTTGCACA ACACAAGTAC CTAATCATAG TTTATCTCAC AGACAGCCTG
661AGACAGTTCT TACGGAAACA CCCCAGGACA CAATTGAATT AAACAGATTG AATTTAGAAT
721CTTCCAATTC AAAGTACTCC TTGAATACAG ATTCCTCAGT GTCTTATATT GATTCAGCTG
781TAATTTCACC TGATACTGTC CCACTGGGAA CAGGAACTTC CATATTATCT AAACAGGTTC
841AAAATAAACC AAAAACTGGT CGAAGTTTAT TAGGAGGACC AGCAGCTCTT AGTCCATTAA
901CCCCAAGTTT TGGGATTTTG CCATTAGAAA CCCCAAGTCC TGGAGATGGA TCCTATTTAC
961AAAACTACAC TAATACACCT CCTGTAATTG ATGTGCCATC CACCGGAGCC CCTTCAAAAA
1021AGACTTTTCG TGTTTTACAG TCTGTTGCCA GAATCGGCCA AACTGGAACA AAGTCTGTCT
1081TCTCACAGAG TGGAAATAGC CGAGAGGTAA CTCCAATTCT TGCACAAACA CAAAGTTCTG
1141GTCCACAAAC AAGTACAACA CCTCAGGTAT TGAGCCCCAC TATTACATCT CCCCCAAACG
1201CACTGCCTCG AAGAAGTTCA CGACTCTTTA CTAGTGACAG CTCCACAACC AAGGAGAATA
1261GCAAAAAATT AAAAATGAAG TTTCCACCTG AAATCCCAAA CAGAAAAACA AAAAGTAAAA
1321CTAATAAAGG AGGAATAACT CAACCTAACA TAAATGATAG CCTGGAAATT ACAAAATTGG
1381ACTCTTCCAT CATTTCAGAA GGGAAAATAT CCACAATCAC ACCTCAGATT CAGGCCTTTA
1441ATCTACAAAA AGCAGCAGCA GAAGGTTTGA TGAGCCTTCT TCGTGAAATG GGGAAAGGTT
1501ATTTAGCTTT GTGTTCATAC AACTGCAAAG AAGCTATAAA TATTTTGAGC CATCTACCTT
1561CTCACCACTA CAATACTGGT TGGGTACTGT GCCAAATTGG AAGGGCCTAT TTTGAACTTT
1621CAGAGTACAT GCAAGCTGAA AGAATATTCT CAGAGGTTAG AAGGATTGAG AATTATAGAG
1681TTGAAGGCAT GGAGATCTAC TCTACAACAC TTTGGCATCT TCAAAAAGAT GTTGCTCTTT
1741CAGTTCTGTC AAAAGACTTA ACAGACATGG ATAAAAATTC GCCAGAGGCC TGGTGTGCTG
1801CAGGGAACTG TTTCAGTCTG CAACGGGAAC ATGATATTGC AATTAAATTC TTCCAGAGAG
1861CTATCCAAGT TGATCCAAAT TACGCTTATG CCTATACTCT ATTAGGGCAT GAGTTTGTCT
1921TAACTGAAGA ATTGGACAAA GCATTAGCTT GTTTTCGAAA TGCTATCAGA GTCAATCCTA
1981GACATTATAA TGCATGGTAT GGTTTAGGAA TGATTTATTA CAAGCAAGAA AAATTCAGCC
2041TTGCAGAAAT GCATTTCCAA AAAGCGCTTG ATATCAACCC TCAAAGTTCA GTTTTACTTT
2101GCCACATTGG AGTAGTTCAA CATGCACTGA AAAAATCAGA GAAGGCTTTG GATACCCTAA
2161ACAAAGCCAT TGTCATTGAT CCCAAGAACC CTCTATGCAA ATTTCACAGA GCCTCAGTTT
2221TATTTGCAAA TGAAAAATAT AAGTCTGCTT TACAAGAACT TGAAGAATTG AAACAAATTG
2281TTCCCAAAGA ATCCCTCGTT TACTTCTTAA TAGGAAAGGT TTACAAGAAG TTAGGTCAAA
2341CGCACCTCGC CCTGATGAAT TTCTCTTGGG CTATGGATTT AGATCCTAAA GGAGCCAATA
2401ACCAGATTAA AGAGGCAATT GATAAGCGTT ATCTTCCAGA TGATGAGGAG CCAATAACCC
2461AAGAAGAACA GATCATGGGA ACAGATGAAT CCCAGGAGAG CAGCATGACA GATGCGGATG
2521ACACACAACT TCATGCAGCT GAAAGTGATG AATTTTACCC AACTTTCTTG TACAAAGTtg
2581gcattataag aaagcattgc ttatcaattt gttgcaacga ac

Download FASTA (ORF) (Full)