Construct: ORF ccsbBroadEn_06080

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010025.1_s300c1, BRDN0000396940
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DAD1 (1603)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1603 DAD1 defender against cell death 1 NM_001344.4 99.7% 99.1% 59C>T
2 mouse 13135 Dad1 defender against cell death 1 NM_001113358.1 91.1% 99.1% (many diffs)
3 mouse 13135 Dad1 defender against cell death 1 NM_010015.4 91.1% 99.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
405
ORF length:
339
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTC GGCGTCGGTA GTGTCTGTCA TTTCGCGGTT CTTAGAAGAG TACTTGAGCT
121CCATTCCGCA GCGTCTGAAG TTGCTGGACG CGTACCTGCT GTATATACTG CTGACCGGGG
181CGCTGCAGTT CGGTTACTGT CTCCTCGTGG GGACCTTCCC CTTCAACTCT TTTCTCTCGG
241GCTTCATCTC TTGTGTGGGG AGTTTCATCC TAGCGGTTTG CCTGAGAATA CAGATCAACC
301CACAGAACAA AGCGGATTTC CAAGGCATCT CCCCAGAGCG AGCCTTTGCT GATTTTCTCT
361TTGCCAGCAC CATCCTGCAC CTTGTTGTCA TGAACTTTGT TGGCTGCCTA ACTTTCTTGT
421ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)