Construct: ORF ccsbBroadEn_06172

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003745.1_s300c1, BRDN0000396884
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EPS8 (2059)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2059 EPS8 epidermal growth factor rec... NM_004447.6 99.9% 99.8% 1892C>T
2 human 2059 EPS8 epidermal growth factor rec... XM_024448879.1 99.9% 99.8% 1892C>T
3 human 2059 EPS8 epidermal growth factor rec... XM_024448880.1 99.9% 99.8% 1892C>T
4 human 2059 EPS8 epidermal growth factor rec... XM_024448881.1 99.9% 99.8% 1892C>T
5 human 2059 EPS8 epidermal growth factor rec... XM_024448882.1 99.9% 99.8% 1892C>T
6 human 2059 EPS8 epidermal growth factor rec... XM_024448878.1 98.6% 98.5% 1_33del;1925C>T
7 human 2059 EPS8 epidermal growth factor rec... XM_011520605.3 97.5% 97.5% 1_60del;1952C>T
8 mouse 13860 Eps8 epidermal growth factor rec... NM_001271588.1 84.4% 88.9% (many diffs)
9 mouse 13860 Eps8 epidermal growth factor rec... NM_001271595.1 84.4% 88.9% (many diffs)
10 mouse 13860 Eps8 epidermal growth factor rec... NM_007945.3 84.4% 88.9% (many diffs)
11 mouse 13860 Eps8 epidermal growth factor rec... XM_011241204.2 84.4% 88.9% (many diffs)
12 mouse 13860 Eps8 epidermal growth factor rec... XM_017321383.1 83% 86.9% (many diffs)
13 mouse 13860 Eps8 epidermal growth factor rec... NM_001271587.1 81.8% 86.2% (many diffs)
14 mouse 13860 Eps8 epidermal growth factor rec... XM_006505508.3 81.5% 86.3% (many diffs)
15 mouse 13860 Eps8 epidermal growth factor rec... NM_001271589.1 57.2% 60.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2532
ORF length:
2466
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAA TGGTCATATT TCTAATCATC CCAGTAGTTT TGGAATGTAC CCATCTCAGA
121TGAATGGCTA CGGATCATCA CCTACCTTTT CCCAGACGGA CAGAGAACAT GGTTCAAAAA
181CAAGTGCAAA GGCCCTTTAT GAACAAAGGA AGAATTATGC ACGGGACAGT GTCAGCAGTG
241TGTCAGATAT ATCTCAATAC CGTGTTGAAC ACTTGACTAC CTTTGTCCTG GATCGGAAAG
301ATGCTATGAT CACTGTTGAT GATGGAATAA GGAAATTGAA ATTGCTTGAT GCCAAGGGCA
361AAGTGTGGAC TCAAGATATG ATTCTTCAAG TGGATGACAG AGCTGTGAGC CTGATTGATT
421TAGAATCAAA GAATGAACTG GAGAATTTTC CTTTAAACAC AATCCAGCAC TGCCAAGCTG
481TGATGCATTC ATGCAGCTAT GATTCAGTTC TTGCACTGGT GTGCAAAGAG CCAACCCAGA
541ACAAGCCAGA TCTTCATCTC TTCCAGTGTG ATGAGGTTAA GGCAAACCTA ATTAGTGAAG
601ATATTGAAAG TGCAATCAGT GACAGTAAAG GAGGGAAACA GAAGAGGCGG CCCGACGCCC
661TGAGGATGAT TTCCAATGCA GACCCTAGTA TACCGCCTCC ACCCAGAGCT CCTGCCCCTG
721CGCCCCCTGG GACCGTCACC CAGGTGGATG TTAGAAGTCG AGTGGCAGCC TGGTCTGCAT
781GGGCAGCCGA CCAAGGGGAC TTTGAGAAAC CAAGGCAGTA TCATGAGCAG GAAGAAACAC
841CTGAGATGAT GGCAGCCCGC ATTGACAGAG ATGTGCAAAT CTTAAACCAC ATTTTGGATG
901ACATTGAATT TTTTATCACA AAACTCCAAA AAGCAGCAGA AGCATTTTCT GAGCTTTCTA
961AAAGGAAGAA AAACAAGAAA GGTAAAAGGA AAGGACCAGG AGAGGGTGTT TTAACGCTGC
1021GGGCAAAACC TCCACCTCCT GATGAATTTC TTGACTGTTT CCAAAAGTTT AAACACGGAT
1081TTAACCTTCT GGCCAAACTG AAGTCTCATA TTCAGAATCC TAGTGCTGCA GATTTGGTTC
1141ACTTTTTGTT TACTCCATTA AATATGGTGG TGCAGGCAAC AGGAGGTCCT GAACTAGCCA
1201GTTCAGTACT TAGTCCCCTA TTGAATAAGG ACACAATTGA TTTCTTAAAT TATACTGTCA
1261ATGGTGATGA ACGGCAGCTG TGGATGTCAT TGGGAGGAAC TTGGATGAAA GCCAGAGCAG
1321AGTGGCCAAA AGAACAGTTT ATTCCACCAT ATGTTCCACG ATTCCGCAAT GGCTGGGAGC
1381CCCCAATGCT GAACTTTATG GGAGCCACAA TGGAACAAGA TCTTTATCAA CTGGCAGAAT
1441CTGTGGCAAA TGTAGCAGAA CATCAGCGCA AACAGGAAAT AAAAAGATTA TCCACAGAGC
1501ATTCCAGTGT ATCAGAGTAT CATCCAGCCG ATGGCTATGC GTTCAGTAGC AACATTTACA
1561CAAGAGGATC CCACCTGGAC CAAGGGGAAG CTGCTGTTGC TTTTAAGCCA ACTTCTAATC
1621GCCATATAGA TAGAAATTAT GAACCACTCA AAACACAACC CAAGAAATAT GCCAAATCCA
1681AGTATGACTT TGTAGCAAGG AACAACAGTG AGCTCTCGGT TCTAAAGGAT GATATTTTAG
1741AGATACTTGA TGATCGGAAG CAATGGTGGA AAGTTCGAAA TGCAAGTGGA GACTCTGGAT
1801TTGTGCCAAA TAACATTTTG GATATTGTGA GACCTCCAGA ATCTGGATTG GGGCGTGCTG
1861ATCCACCTTA TACTCATACT ATACAGAAAC AAAGGATGGA GTATGGCCCA AGACCAGCTG
1921ATACTCCCCC TGCTCCATCA CCTCCTCCAA CACCAGTTCC TGTTCCTGTT CCCCTTCCCC
1981CTTCCACTCC AGCACCTGTT CCTGTGTCAA AGGTCCCAGC AAATATAACA CGTCAAAACA
2041GCAGCTCCAG TGACAGTGGT GGCAGTATCG TGCGAGACAG CCAGAGACAC AAACAACTTC
2101CGGTGGACCG AAGGAAATCT CAGATGGAGG AAGTGCAAGA TGAACTCATC CACAGACTGA
2161CCATTGGTCG GAGTGCCGCT CAGAAGAAAT TCCATGTGCC ACGGCAGAAC GTGCCAGTTA
2221TCAATATCAC TTACGACTCC ACACCAGAGG ATGTGAAGAC GTGGTTACAG TCAAAGGGAT
2281TCAACCCTGT GACTGTCAAT AGTCTTGGAG TATTAAATGG TGCACAACTT TTCTCTCTCA
2341ATAAGGATGA ACTGAGGACA GTCTGCCCTG AAGGGGCGAG AGTCTATAGC CAAATCACTG
2401TACAAAAAGC TGCATTGGAG GATAGCAGTG GCAGCTCCGA GTTACAAGAA ATTATGAGAA
2461GACGACAGGA AAAAATCAGT GCTGCCGCTA GTGATTCAGG AGTGGAATCT TTTGATGAAG
2521GAAGCAGTCA CTACCCAACT TTCTTGTACA AAGTtggcat tataagaaag cattgcttat
2581caatttgttg caacgaac

Download FASTA (ORF) (Full)