Construct: ORF ccsbBroadEn_06251

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017523.1_s300c1, BRDN0000389269
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GALK2 (2585)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2585 GALK2 galactokinase 2 NM_002044.4 99.9% 100% 1221C>T
2 human 2585 GALK2 galactokinase 2 XM_005254279.4 98% 96.9% (many diffs)
3 human 2585 GALK2 galactokinase 2 XM_006720461.4 97.6% 97.1% (many diffs)
4 human 2585 GALK2 galactokinase 2 NM_001001556.3 96.3% 96.2% (many diffs)
5 human 2585 GALK2 galactokinase 2 NM_001289030.2 94.6% 94.7% 0_1ins72;1149C>T
6 human 2585 GALK2 galactokinase 2 NM_001289031.1 94.6% 94.7% 0_1ins72;1149C>T
7 human 2585 GALK2 galactokinase 2 XM_005254280.4 94.4% 93.3% (many diffs)
8 human 2585 GALK2 galactokinase 2 XM_006720462.4 92.8% 91.8% (many diffs)
9 human 2585 GALK2 galactokinase 2 XM_024449892.1 92.8% 91.8% (many diffs)
10 human 2585 GALK2 galactokinase 2 XM_006720463.4 90.9% 89.9% (many diffs)
11 human 2585 GALK2 galactokinase 2 XM_017022062.2 87.4% 87.5% 0_1ins72;432_433ins99;1050C>T
12 human 2585 GALK2 galactokinase 2 XM_005254284.4 87.4% 86.2% (many diffs)
13 human 2585 GALK2 galactokinase 2 XM_024449893.1 86.9% 86.3% (many diffs)
14 human 2585 GALK2 galactokinase 2 XM_024449894.1 85.7% 84.7% (many diffs)
15 human 2585 GALK2 galactokinase 2 XM_006720464.4 79.9% 78.9% (many diffs)
16 human 2585 GALK2 galactokinase 2 XM_017022063.2 76.4% 75.3% (many diffs)
17 human 2585 GALK2 galactokinase 2 XM_011521441.3 70.2% 69.3% (many diffs)
18 human 2585 GALK2 galactokinase 2 XM_017022064.2 70.2% 69.3% (many diffs)
19 human 2585 GALK2 galactokinase 2 NM_001352047.1 69.7% 69.3% (many diffs)
20 human 2585 GALK2 galactokinase 2 NM_001352048.1 69.7% 69.3% (many diffs)
21 human 2585 GALK2 galactokinase 2 XM_024449895.1 64.5% 64.6% 0_1ins387;117_118ins99;735C>T
22 human 2585 GALK2 galactokinase 2 XR_001751207.2 63.6% 1_91del;1058_1227del;1302_1303ins333
23 human 2585 GALK2 galactokinase 2 XR_001751204.2 33% (many diffs)
24 human 2585 GALK2 galactokinase 2 XR_001751202.2 32.3% (many diffs)
25 human 2585 GALK2 galactokinase 2 XR_001751205.2 31.9% (many diffs)
26 human 2585 GALK2 galactokinase 2 XR_002957633.1 31.3% (many diffs)
27 human 2585 GALK2 galactokinase 2 XR_001751206.2 31% (many diffs)
28 human 2585 GALK2 galactokinase 2 XR_001751208.2 30% (many diffs)
29 human 2585 GALK2 galactokinase 2 NR_147895.2 26.2% (many diffs)
30 mouse 69976 Galk2 galactokinase 2 NM_001291002.1 84.9% 88.8% (many diffs)
31 mouse 69976 Galk2 galactokinase 2 NM_175154.3 82% 85% (many diffs)
32 mouse 69976 Galk2 galactokinase 2 XM_006500153.3 80.6% 84% (many diffs)
33 mouse 69976 Galk2 galactokinase 2 XM_011239770.2 80.6% 84% (many diffs)
34 mouse 69976 Galk2 galactokinase 2 XM_006500154.1 69.8% 70% (many diffs)
35 mouse 69976 Galk2 galactokinase 2 XM_011239772.1 49.7% 51% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1440
ORF length:
1374
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC TACAGAGAGC CCTGCTACGC GTCGGGTCCA GGTGGCAGAA CATCCTAGGT
121TACTGAAGCT AAAGGAGATG TTTAACTCCA AGTTTGGATC TATTCCCAAG TTTTATGTTC
181GAGCACCAGG AAGAGTCAAC ATAATAGGAG AGCATATAGA TTATTGTGGA TATTCTGTTC
241TTCCTATGGC TGTAGAACAA GATGTGCTAA TAGCTGTAGA ACCTGTGAAA ACGTACGCTC
301TCCAACTGGC CAATACAAAT CCCTTGTATC CGGACTTCAG TACTAGTGCT AATAACATCC
361AGATTGATAA AACCAAGCCT TTGTGGCACA ACTATTTCTT ATGTGGACTT AAAGGAATTC
421AGGAACACTT TGGTCTTAGT AACCTGACTG GAATGAACTG CCTGGTAGAT GGAAATATCC
481CACCAAGTTC TGGCCTCTCC AGCTCCAGTG CTTTGGTCTG TTGTGCTGGC TTGGTGACGC
541TCACAGTGCT GGGAAGGAAT CTATCCAAGG TGGAACTTGC AGAAATCTGT GCCAAGAGTG
601AGCGTTACAT TGGCACTGAA GGAGGAGGCA TGGACCAGTC TATATCATTT CTTGCAGAAG
661AAGGAACTGC CAAGTTGATA GAATTTAGTC CTCTGAGGGC AACCGATGTA AAACTCCCAA
721GTGGAGCAGT GTTTGTGATT GCCAACAGTT GTGTGGAGAT GAATAAGGCA GCAACTTCCC
781ATTTCAATAT CAGGGTGATG GAGTGTCGGC TGGCTGCGAA GCTCCTGGCT AAATACAAAA
841GCTTGCAATG GGACAAAGTA CTGAGGCTGG AGGAGGTGCA GGCTAAACTA GGGATTAGTC
901TAGAAGAAAT GCTGTTGGTC ACAGAAGATG CCCTTCATCC TGAACCCTAT AACCCTGAGG
961AGATCTGCAG GTGTCTGGGA ATTAGCCTGG AGGAACTCCG AACCCAAATC CTGAGTCCAA
1021ACACTCAAGA TGTGCTCATC TTCAAACTCT ATCAGCGGGC AAAGCATGTG TACAGCGAGG
1081CTGCGCGAGT GCTCCAGTTT AAGAAGATAT GTGAAGAAGC ACCTGAAAAC ATGGTCCAGC
1141TGCTGGGAGA GTTGATGAAC CAGAGCCACA TGAGCTGCCG GGACATGTAT GAGTGCAGCT
1201GCCCCGAGCT GGATCAGCTG GTGGACATCT GTCGGAAGTT TGGGGCTCAA GGGTCACGAC
1261TTACTGGAGC AGGATGGGGA GGCTGTACAG TATCAATGGT ACCTGCGGAC AAGCTGCCCA
1321GCTTTCTAGC AAATGTGCAC AAAGCTTATT ACCAGAGGAG TGATGGAAGC TTAGCACCGG
1381AGAAGCAAAG TTTGTTTGCT ACCAAACCTG GAGGTGGGGC TTTGGTTTTG CTTGAGGCCT
1441GCCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca
1501acgaac

Download FASTA (ORF) (Full)