Transcript: Mouse XM_006500153.3

PREDICTED: Mus musculus galactokinase 2 (Galk2), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Galk2 (69976)
Length:
1934
CDS:
305..1609

Additional Resources:

NCBI RefSeq record:
XM_006500153.3
NBCI Gene record:
Galk2 (69976)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006500153.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000024642 CCAAGGTGGAACTTGCAGAAA pLKO.1 732 CDS 100% 4.950 3.465 N Galk2 n/a
2 TRCN0000278674 CCAAGGTGGAACTTGCAGAAA pLKO_005 732 CDS 100% 4.950 3.465 N Galk2 n/a
3 TRCN0000024639 GCACATAGATTACTGTGGATA pLKO.1 379 CDS 100% 4.950 3.465 N Galk2 n/a
4 TRCN0000278677 GCACATAGATTACTGTGGATA pLKO_005 379 CDS 100% 4.950 3.465 N Galk2 n/a
5 TRCN0000024640 CCTGGAATGAATTGCCTGGTA pLKO.1 614 CDS 100% 2.640 1.848 N Galk2 n/a
6 TRCN0000278675 CCTGGAATGAATTGCCTGGTA pLKO_005 614 CDS 100% 2.640 1.848 N Galk2 n/a
7 TRCN0000024641 GCATAGCTTGTTTGCCACCAA pLKO.1 1552 CDS 100% 2.640 1.848 N Galk2 n/a
8 TRCN0000278673 GCATAGCTTGTTTGCCACCAA pLKO_005 1552 CDS 100% 2.640 1.848 N Galk2 n/a
9 TRCN0000024643 CTACAGCTAATAACATCTGTA pLKO.1 510 CDS 100% 0.495 0.347 N Galk2 n/a
10 TRCN0000278608 CTACAGCTAATAACATCTGTA pLKO_005 510 CDS 100% 0.495 0.347 N Galk2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006500153.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06251 pDONR223 100% 80.6% 84% None (many diffs) n/a
2 ccsbBroad304_06251 pLX_304 0% 80.6% 84% V5 (many diffs) n/a
3 TRCN0000471462 GAGCCGTCTCTGATTGCCTGGCAG pLX_317 32.5% 80.6% 84% V5 (many diffs) n/a
4 ccsbBroadEn_14652 pDONR223 0% 80.6% 84% None (many diffs) n/a
5 ccsbBroad304_14652 pLX_304 0% 80.6% 84% V5 (many diffs) n/a
6 TRCN0000481375 GTTCAACTCTAATCACCCACGGGC pLX_317 29.7% 80.6% 84% V5 (many diffs) n/a
7 TRCN0000489439 TGGGGACTCGCATTTTCGGGAGAG pLX_317 29.5% 80.6% 84% V5 (not translated due to prior stop codon) (many diffs) n/a
8 TRCN0000492356 ATTAACTTACTCAAGTGTGTTCCT pLX_317 29.3% 80.5% 83.8% V5 (many diffs) n/a
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