Construct: ORF ccsbBroadEn_06292

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012503.1_s300c1, BRDN0000385198
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GNAI2 (2771)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2771 GNAI2 G protein subunit alpha i2 NM_002070.4 99.9% 99.7% 125G>T
2 human 2771 GNAI2 G protein subunit alpha i2 NM_001282620.2 92.1% 89.8% (many diffs)
3 human 2771 GNAI2 G protein subunit alpha i2 NM_001282619.2 90.8% 88% (many diffs)
4 human 2771 GNAI2 G protein subunit alpha i2 NM_001166425.2 89.2% 88.7% (many diffs)
5 human 2771 GNAI2 G protein subunit alpha i2 NM_001282617.1 85.3% 85.3% 0_1ins156
6 human 2771 GNAI2 G protein subunit alpha i2 NM_001282618.2 77.1% 77.1% 0_1ins243
7 mouse 14678 Gnai2 guanine nucleotide binding ... NM_008138.5 91.6% 98% (many diffs)
8 mouse 14678 Gnai2 guanine nucleotide binding ... XM_006511637.3 91.6% 98% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1131
ORF length:
1065
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGG CTGCACCGTG AGCGCCGAGG ACAAGGCGGC GGCCGAGCGC TCTAAGATGA
121TCGACAAGAA CCTGCGGGAG GACGGAGAGA AGGCGGCGCG GGAGGTGAAG TTGCTGCTGT
181TGGGTGCTGT GGAGTCAGGG AAGAGCACCA TCGTCAAGCA GATGAAGATC ATCCACGAGG
241ATGGCTACTC CGAGGAGGAA TGCCGGCAGT ACCGGGCGGT TGTCTACAGC AACACCATCC
301AGTCCATCAT GGCCATTGTC AAAGCCATGG GCAACCTGCA GATCGACTTT GCCGACCCCT
361CCAGAGCGGA CGACGCCAGG CAGCTATTTG CACTGTCCTG CACCGCCGAG GAGCAAGGCG
421TGCTCCCTGA TGACCTGTCC GGCGTCATCC GGAGGCTCTG GGCTGACCAT GGTGTGCAGG
481CCTGCTTTGG CCGCTCAAGG GAATACCAGC TCAACGACTC AGCTGCCTAC TACCTGAACG
541ACCTGGAGCG TATTGCACAG AGTGACTACA TCCCCACACA GCAAGATGTG CTACGGACCC
601GCGTAAAGAC CACGGGGATC GTGGAGACAC ACTTCACCTT CAAGGACCTA CACTTCAAGA
661TGTTTGATGT GGGTGGTCAG CGGTCTGAGC GGAAGAAGTG GATCCACTGC TTTGAGGGCG
721TCACAGCCAT CATCTTCTGC GTAGCCTTGA GCGCCTATGA CTTGGTGCTA GCTGAGGACG
781AGGAGATGAA CCGCATGCAT GAGAGCATGA AGCTATTCGA TAGCATCTGC AACAACAAGT
841GGTTCACAGA CACGTCCATC ATCCTCTTCC TCAACAAGAA GGACCTGTTT GAGGAGAAGA
901TCACACACAG TCCCCTGACC ATCTGCTTCC CTGAGTACAC AGGGGCCAAC AAATATGATG
961AGGCAGCCAG CTACATCCAG AGTAAGTTTG AGGACCTGAA TAAGCGCAAA GACACCAAGG
1021AGATCTACAC GCACTTCACG TGCGCCACCG ACACCAAGAA CGTGCAGTTC GTGTTTGACG
1081CCGTCACCGA TGTCATCATC AAGAACAACC TGAAGGACTG CGGCCTCTTC TGCCCAACTT
1141TCTTGTACAA AGTtggcatt ataagaaagc attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)