Construct: ORF ccsbBroadEn_06306

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005548.1_s300c1, BRDN0000386820
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GPM6A (2823)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2823 GPM6A glycoprotein M6A NM_005277.4 99.8% 99.6% 694C>A
2 human 2823 GPM6A glycoprotein M6A NM_201591.2 99.8% 99.6% 694C>A
3 human 2823 GPM6A glycoprotein M6A NM_001261448.1 97% 95.3% (many diffs)
4 human 2823 GPM6A glycoprotein M6A NM_201592.2 95.6% 95.3% 0_1ins33;2_3delTGinsAA;661C>A
5 human 2823 GPM6A glycoprotein M6A XM_006714183.1 77.2% 76.9% 0_1ins189;505C>A
6 human 2823 GPM6A glycoprotein M6A XM_011531877.1 77.2% 76.9% 0_1ins189;505C>A
7 human 2823 GPM6A glycoprotein M6A NM_001261447.1 68% 55% (many diffs)
8 mouse 234267 Gpm6a glycoprotein m6a NM_153581.6 91.8% 98.5% (many diffs)
9 mouse 234267 Gpm6a glycoprotein m6a NM_001253754.1 87.8% 94.2% (many diffs)
10 mouse 234267 Gpm6a glycoprotein m6a NM_001253756.1 59.7% 63.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
900
ORF length:
834
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA AGAGAATATG GAAGAGGGAC AGACACAAAA AGGGTGTTTT GAATGCTGTA
121TCAAATGCCT GGGGGGCATT CCCTATGCCT CTCTGATTGC CACCATCCTG CTCTATGCGG
181GTGTTGCCCT GTTCTGTGGC TGCGGTCATG AAGCGCTTTC TGGAACTGTC AACATTCTGC
241AAACCTACTT TGAGATGGCA AGAACTGCTG GAGACACACT GGATGTTTTT ACCATGATTG
301ACATCTTTAA GTATGTGATC TACGGCATCG CAGCTGCGTT CTTTGTGTAT GGCATTTTGC
361TGATGGTGGA AGGTTTCTTC ACAACTGGGG CCATCAAAGA TCTCTATGGG GATTTCAAAA
421TCACCACTTG TGGCAGATGT GTGAGCGCTT GGTTCATTAT GCTGACATAT CTTTTCATGT
481TGGCCTGGCT GGGAGTCACG GCTTTCACCT CACTGCCAGT TTACATGTAC TTCAATCTGT
541GGACCATCTG CCGGAACACC ACATTAGTGG AGGGAGCAAA TCTCTGCTTG GACCTTCGTC
601AGTTTGGAAT TGTGACAATT GGAGAGGAAA AGAAAATTTG TACTGTCTCT GAGAATTTCT
661TGAGGATGTG CGAATCTACT GAGCTGAACA TGACCTTCCA CTTGTTTATT GTGGCACTTG
721CTGGAGCTGG GGCAGCAGTC ATTGCTATGG TTCACTACAT TATGGTTCTG TCTGCCAACT
781GGGCCTATGT GAAAGACGCC TGCCGGATGC AGAAGTATGA AGACATCAAG TCGAAGGAAG
841AGCAAGAGCT TCATGACATC CACTCTACTC GCTCCAAAGA GCGGCTCAAT GCATACACAT
901ACCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca
961acgaac

Download FASTA (ORF) (Full)