Construct: ORF ccsbBroadEn_06329
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007691.1_s300c1, BRDN0000456246
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRPR (2925)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2925 | GRPR | gastrin releasing peptide r... | NM_005314.3 | 99.8% | 100% | 453T>C;663T>C |
| 2 | mouse | 14829 | Grpr | gastrin releasing peptide r... | NM_008177.3 | 87.5% | 89.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1221
- ORF length:
- 1152
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGCTCTAAAT GACTGTTTCC TTCTGAACTT GGAGGTGGAC CATTTCATGC 121 ACTGCAACAT CTCCAGTCAC AGTGCGGATC TCCCCGTGAA CGATGACTGG TCCCACCCGG 181 GGATCCTCTA TGTCATCCCT GCAGTTTATG GGGTTATCAT TCTGATAGGC CTCATTGGCA 241 ACATCACTTT GATCAAGATC TTCTGTACAG TCAAGTCCAT GCGAAACGTT CCAAACCTGT 301 TCATTTCCAG TCTGGCTTTG GGAGACCTGC TCCTCCTAAT AACGTGTGCT CCAGTGGATG 361 CCAGCAGGTA CCTGGCTGAC AGATGGCTAT TTGGCAGGAT TGGCTGCAAA CTGATCCCCT 421 TTATACAGCT TACCTCTGTT GGGGTGTCTG TCTTCACACT CACGGCGCTC TCGGCAGACA 481 GATACAAAGC CATTGTCCGG CCAATGGATA TCCAGGCCTC CCATGCCCTG ATGAAGATCT 541 GCCTCAAAGC CGCCTTTATC TGGATCATCT CCATGCTGCT GGCCATTCCA GAGGCCGTGT 601 TTTCTGACCT CCATCCCTTC CATGAGGAAA GCACCAACCA GACCTTCATT AGCTGTGCCC 661 CATACCCACA CTCTAATGAG CTTCACCCCA AAATCCATTC TATGGCTTCC TTTCTGGTCT 721 TCTACGTCAT CCCACTGTCG ATCATCTCTG TTTACTACTA CTTCATTGCT AAAAATCTGA 781 TCCAGAGTGC TTACAATCTT CCCGTGGAAG GGAATATACA TGTCAAGAAG CAGATTGAAT 841 CCCGGAAGCG ACTTGCCAAG ACAGTGCTGG TGTTTGTGGG CCTGTTCGCC TTCTGCTGGC 901 TCCCCAATCA TGTCATCTAC CTGTACCGCT CCTACCACTA CTCTGAGGTG GACACCTCCA 961 TGCTCCACTT TGTCACCAGC ATCTGTGCCC GCCTCCTGGC CTTCACCAAC TCCTGCGTGA 1021 ACCCCTTTGC CCTCTACCTG CTGAGCAAGA GTTTCAGGAA ACAGTTCAAC ACTCAGCTGC 1081 TCTGTTGCCA GCCTGGCCTG ATCATCCGGT CTCACAGCAC TGGAAGGAGT ACAACCTGCA 1141 TGACCTCCCT CAAGAGTACC AACCCCTCCG TGGCCACCTT TAGCCTCATC AATGGAAACA 1201 TCTGTCACGA GCGGTATGTC TTGCCAACTT TCTTGTACAA AGTTGGcatt ataagaaagc 1261 attgcttatc aatttgttgc aacgaac