Construct: ORF ccsbBroadEn_06339
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010426.1_s300c1, BRDN0000388963
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GTF2F2 (2963)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2963 | GTF2F2 | general transcription facto... | NM_004128.3 | 99.7% | 100% | 102C>T;621G>A |
| 2 | human | 2963 | GTF2F2 | general transcription facto... | XM_011535052.3 | 90.3% | 90.5% | 102C>T;303_380del;699G>A |
| 3 | human | 2963 | GTF2F2 | general transcription facto... | XM_017020551.1 | 59.5% | 59.4% | 0_1ins299;3_3delGinsCA;321G>A |
| 4 | human | 2963 | GTF2F2 | general transcription facto... | XM_011535053.3 | 48.2% | 43.7% | (many diffs) |
| 5 | mouse | 68705 | Gtf2f2 | general transcription facto... | NM_026816.3 | 89.8% | 97.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 813
- ORF length:
- 747
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC CGAGCGCGGG GAACTCGACT TGACCGGCGC CAAACAGAAC ACAGGAGTGT 121 GGCTAGTCAA GGTTCCTAAA TATTTGTCAC AGCAATGGGC TAAAGCTTCT GGAAGAGGTG 181 AAGTTGGGAA ACTGCGGATT GCCAAGACTC AAGGAAGGAC TGAGGTGTCA TTTACTTTGA 241 ATGAGGATCT TGCAAATATT CATGATATTG GTGGAAAACC AGCTTCAGTC AGTGCTCCTA 301 GAGAACATCC ATTTGTCTTG CAAAGTGTTG GAGGACAGAC ATTAACAGTA TTTACTGAGA 361 GCTCATCAGA TAAGCTGTCA TTGGAAGGAA TAGTGGTACA AAGAGCTGAA TGCCGACCAG 421 CTGCCAGTGA AAACTACATG CGATTAAAAA GATTGCAAAT AGAAGAGTCT TCCAAACCAG 481 TGAGGCTATC ACAACAGCTG GACAAAGTTG TAACAACCAA TTACAAACCT GTTGCTAATC 541 ATCAATACAA TATCGAATAT GAAAGGAAAA AGAAAGAAGA CGGAAAGCGA GCTCGAGCTG 601 ATAAACAACA TGTTTTAGAC ATGCTATTTT CAGCCTTTGA GAAACATCAA TACTATAATC 661 TTAAGGACTT GGTGGACATC ACAAAACAAC CTGTGGTGTA CCTGAAGGAA ATCTTAAAAG 721 AAATTGGTGT TCAGAATGTA AAAGGGATCC ACAAAAACAC ATGGGAGCTG AAGCCAGAGT 781 ACAGACACTA TCAAGGAGAA GAAAAGAGTG ACTACCCAAC TTTCTTGTAC AAAGTtggca 841 ttataagaaa gcattgctta tcaatttgtt gcaacgaac