Construct: ORF ccsbBroadEn_06343

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000201.1_s300c1, BRDN0000389880
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GUCY1B1 (2983)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2983 GUCY1B1 guanylate cyclase 1 soluble... NM_000857.4 99.9% 100% 1047G>A
2 human 2983 GUCY1B1 guanylate cyclase 1 soluble... NM_001291951.2 96.5% 96.5% 298_363del;1113G>A
3 human 2983 GUCY1B1 guanylate cyclase 1 soluble... NM_001291952.2 96.4% 95.7% (many diffs)
4 human 2983 GUCY1B1 guanylate cyclase 1 soluble... NM_001291954.2 94.6% 94.6% 1047G>A;1175_1176ins99
5 human 2983 GUCY1B1 guanylate cyclase 1 soluble... XM_011531901.1 93.4% 93.5% 1047G>A;1176_1304del
6 human 2983 GUCY1B1 guanylate cyclase 1 soluble... NM_001291955.2 88.9% 89% 0_1ins204;843G>A
7 human 2983 GUCY1B1 guanylate cyclase 1 soluble... XM_017008132.1 88.9% 89% 0_1ins204;843G>A
8 human 2983 GUCY1B1 guanylate cyclase 1 soluble... XM_017008133.1 88.9% 89% 0_1ins204;843G>A
9 human 2983 GUCY1B1 guanylate cyclase 1 soluble... NM_001291953.2 83.1% 83.2% 0_1ins204;843G>A;972_1100del
10 human 2983 GUCY1B1 guanylate cyclase 1 soluble... XM_017008130.1 83.1% 83.2% 0_1ins204;843G>A;972_1100del
11 human 2983 GUCY1B1 guanylate cyclase 1 soluble... XM_017008131.2 83.1% 83.2% 0_1ins204;843G>A;972_1100del
12 mouse 54195 Gucy1b3 guanylate cyclase 1, solubl... NM_017469.4 88.5% 98.7% (many diffs)
13 mouse 54195 Gucy1b3 guanylate cyclase 1, solubl... NM_001161796.1 87.3% 92.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1923
ORF length:
1857
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTA CGGATTTGTG AATCACGCCC TGGAGTTGCT GGTGATCCGC AATTACGGCC
121CCGAGGTGTG GGAAGACATC AAAAAAGAGG CACAGTTAGA TGAAGAAGGA CAGTTTCTTG
181TCAGAATAAT ATATGATGAC TCCAAAACTT ATGATTTGGT TGCTGCTGCA AGCAAAGTCC
241TCAATCTCAA TGCTGGAGAA ATCCTCCAAA TGTTTGGGAA GATGTTTTTC GTCTTTTGCC
301AAGAATCTGG TTATGATACA ATCTTGCGTG TCCTGGGCTC TAATGTCAGA GAATTTCTAC
361AGAACCTTGA TGCTCTGCAC GACCACCTTG CTACCATCTA CCCAGGAATG CGTGCACCTT
421CCTTTAGGTG CACTGATGCA GAAAAGGGCA AAGGACTCAT TTTGCACTAC TACTCAGAGA
481GAGAAGGACT TCAGGATATT GTCATTGGAA TCATCAAAAC AGTGGCACAA CAAATCCATG
541GCACTGAAAT AGACATGAAG GTTATTCAGC AAAGAAATGA AGAATGTGAT CATACTCAAT
601TTTTAATTGA AGAAAAAGAG TCAAAAGAAG AGGATTTTTA TGAAGATCTT GACAGATTTG
661AAGAAAATGG TACCCAGGAA TCACGCATCA GCCCATATAC ATTCTGCAAA GCTTTTCCTT
721TTCATATAAT ATTTGACCGG GACCTAGTGG TCACTCAGTG TGGCAATGCT ATATACAGAG
781TTCTCCCCCA GCTCCAGCCT GGGAATTGCA GCCTTCTGTC TGTCTTCTCG CTGGTTCGTC
841CTCATATTGA TATTAGTTTC CATGGGATCC TTTCTCACAT CAATACTGTT TTTGTATTGA
901GAAGCAAGGA AGGATTGTTG GATGTGGAGA AATTAGAATG TGAGGATGAA CTGACTGGGA
961CTGAGATCAG CTGCTTACGT CTCAAGGGTC AAATGATCTA CTTACCTGAA GCAGATAGCA
1021TACTTTTTCT ATGTTCACCA AGTGTCATGA ACCTGGACGA TTTGACAAGG AGAGGGCTGT
1081ATCTAAGTGA CATCCCTCTG CATGATGCCA CACGCGATCT TGTTCTTTTG GGAGAACAAT
1141TTAGAGAGGA ATACAAACTC ACCCAAGAAC TGGAAATCCT CACTGACAGG CTACAGCTCA
1201CGTTAAGAGC CCTGGAAGAT GAAAAGAAAA AGACAGACAC ATTGCTGTAT TCTGTCCTTC
1261CTCCGTCTGT TGCCAATGAG CTGCGGCACA AGCGTCCAGT GCCTGCCAAA AGATATGACA
1321ATGTGACCAT CCTCTTTAGT GGCATTGTGG GCTTCAATGC TTTCTGTAGC AAGCATGCAT
1381CTGGAGAAGG AGCCATGAAG ATCGTCAACC TCCTCAACGA CCTCTACACC AGATTTGACA
1441CACTGACTGA TTCCCGGAAA AACCCATTTG TTTATAAGGT GGAGACTGTT GGTGACAAGT
1501ATATGACAGT GAGTGGTTTA CCAGAGCCAT GCATTCACCA TGCACGATCC ATCTGCCACC
1561TGGCCTTGGA CATGATGGAA ATTGCTGGCC AGGTTCAAGT AGATGGTGAA TCTGTTCAGA
1621TAACAATAGG GATACACACT GGAGAGGTAG TTACAGGTGT CATAGGACAG CGGATGCCTC
1681GATACTGTCT TTTTGGGAAT ACTGTCAACC TCACAAGCCG AACAGAAACC ACAGGAGAAA
1741AGGGAAAAAT AAATGTGTCT GAATATACAT ACAGATGTCT TATGTCTCCA GAAAATTCAG
1801ATCCACAATT CCACTTGGAG CACAGAGGCC CAGTGTCCAT GAAGGGCAAA AAAGAACCAA
1861TGCAAGTTTG GTTTCTATCC AGAAAAAATA CAGGAACAGA GGAAACAAAG CAGGATGATG
1921ACTGCCCAAC TTTCTTGTAC AAAGTtggca ttataagaaa gcattgctta tcaatttgtt
1981gcaacgaac

Download FASTA (ORF) (Full)