Construct: ORF ccsbBroadEn_06518
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006622.1_s300c1, BRDN0000390312
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LDHA (3939)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3939 | LDHA | lactate dehydrogenase A | NM_005566.4 | 99.7% | 100% | 483C>T;519A>G |
| 2 | human | 3939 | LDHA | lactate dehydrogenase A | NM_001165414.1 | 91.7% | 91.9% | 1_87del;570C>T;606A>G |
| 3 | human | 3939 | LDHA | lactate dehydrogenase A | NM_001135239.1 | 82.3% | 82.5% | 242_243ins174;309C>T;345A>G |
| 4 | human | 3939 | LDHA | lactate dehydrogenase A | NM_001165415.2 | 77.6% | 71.9% | (many diffs) |
| 5 | human | 3939 | LDHA | lactate dehydrogenase A | NM_001165416.1 | 71.9% | 71% | (many diffs) |
| 6 | human | 3939 | LDHA | lactate dehydrogenase A | NR_028500.1 | 39.3% | (many diffs) | |
| 7 | mouse | 16828 | Ldha | lactate dehydrogenase A | NM_010699.2 | 86.2% | 93.9% | (many diffs) |
| 8 | mouse | 16828 | Ldha | lactate dehydrogenase A | NM_001136069.2 | 79.3% | 86.4% | (many diffs) |
| 9 | mouse | 16828 | Ldha | lactate dehydrogenase A | NR_102727.1 | 45.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1062
- ORF length:
- 996
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC AACTCTAAAG GATCAGCTGA TTTATAATCT TCTAAAGGAA GAACAGACCC 121 CCCAGAATAA GATTACAGTT GTTGGGGTTG GTGCTGTTGG CATGGCCTGT GCCATCAGTA 181 TCTTAATGAA GGACTTGGCA GATGAACTTG CTCTTGTTGA TGTCATCGAA GACAAATTGA 241 AGGGAGAGAT GATGGATCTC CAACATGGCA GCCTTTTCCT TAGAACACCA AAGATTGTCT 301 CTGGCAAAGA CTATAATGTA ACTGCAAACT CCAAGCTGGT CATTATCACG GCTGGGGCAC 361 GTCAGCAAGA GGGAGAAAGC CGTCTTAATT TGGTCCAGCG TAACGTGAAC ATCTTTAAAT 421 TCATCATTCC TAATGTTGTA AAATACAGCC CGAACTGCAA GTTGCTTATT GTTTCAAATC 481 CAGTGGATAT CTTGACCTAC GTGGCTTGGA AGATAAGTGG TTTTCCCAAA AACCGTGTTA 541 TTGGAAGTGG TTGCAATCTG GATTCAGCCC GATTCCGTTA CCTGATGGGG GAAAGGCTGG 601 GAGTTCACCC ATTAAGCTGT CATGGGTGGG TCCTTGGGGA ACATGGAGAT TCCAGTGTGC 661 CTGTATGGAG TGGAATGAAT GTTGCTGGTG TCTCTCTGAA GACTCTGCAC CCAGATTTAG 721 GGACTGATAA AGATAAGGAA CAGTGGAAAG AGGTTCACAA GCAGGTGGTT GAGAGTGCTT 781 ATGAGGTGAT CAAACTCAAA GGCTACACAT CCTGGGCTAT TGGACTCTCT GTAGCAGATT 841 TGGCAGAGAG TATAATGAAG AATCTTAGGC GGGTGCACCC AGTTTCCACC ATGATTAAGG 901 GTCTTTACGG AATAAAGGAT GATGTCTTCC TTAGTGTTCC TTGCATTTTG GGACAGAATG 961 GAATCTCAGA CCTTGTGAAG GTGACTCTGA CTTCTGAGGA AGAGGCCCGT TTGAAGAAGA 1021 GTGCAGATAC ACTTTGGGGG ATCCAAAAGG AGCTGCAATT TTACCCAACT TTCTTGTACA 1081 AAGTtggcat tataagaaag cattgcttat caatttgttg caacgaac