Construct: ORF ccsbBroadEn_06560

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011894.1_s300c1, BRDN0000395439
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MARK3 (4140)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4140 MARK3 microtubule affinity regula... NM_002376.6 100% 100%
2 human 4140 MARK3 microtubule affinity regula... XM_005267641.5 98.7% 98.7% 1843_1869del
3 human 4140 MARK3 microtubule affinity regula... NM_001128919.2 97.9% 97.9% 1843_1887del
4 human 4140 MARK3 microtubule affinity regula... NM_001128920.2 97.8% 97.8% 1108_1109ins48
5 human 4140 MARK3 microtubule affinity regula... NM_001128918.3 96.8% 96.8% 1843_1914del
6 human 4140 MARK3 microtubule affinity regula... XM_017021292.2 96.6% 96.6% 1108_1109ins48;1795_1821del
7 human 4140 MARK3 microtubule affinity regula... XM_017021291.2 95.8% 95.8% 1108_1109ins48;1795_1839del
8 human 4140 MARK3 microtubule affinity regula... XM_024449583.1 94.8% 94.5% (many diffs)
9 human 4140 MARK3 microtubule affinity regula... XM_005267642.5 94.6% 94.6% 1108_1109ins48;1795_1866del
10 human 4140 MARK3 microtubule affinity regula... XM_017021293.1 94.5% 94.3% (many diffs)
11 human 4140 MARK3 microtubule affinity regula... XM_017021298.2 91.2% 91.2% 51_52ins192
12 human 4140 MARK3 microtubule affinity regula... XM_017021296.2 90.1% 90.1% 51_52ins192;1651_1677del
13 human 4140 MARK3 microtubule affinity regula... XM_017021295.2 89.3% 89.3% 51_52ins192;1651_1695del
14 human 4140 MARK3 microtubule affinity regula... XM_017021294.2 88.3% 88.3% 51_52ins192;1651_1722del
15 human 4140 MARK3 microtubule affinity regula... XM_005267643.5 88.2% 88.2% 1584_1585ins258
16 human 4140 MARK3 microtubule affinity regula... NM_001128921.2 88% 88% 537_538ins237;1606_1632del
17 human 4140 MARK3 microtubule affinity regula... XM_017021299.2 87.2% 87.2% 51_52ins192;916_917ins48;1603_1647del
18 human 4140 MARK3 microtubule affinity regula... XM_017021297.2 86.1% 86.1% 51_52ins192;916_917ins48;1603_1674del
19 human 4140 MARK3 microtubule affinity regula... XM_011536769.3 86.1% 86% 0_1ins241;2delT;1603_1674del
20 human 4140 MARK3 microtubule affinity regula... XM_024449587.1 82% 82% 0_1ins393
21 human 4140 MARK3 microtubule affinity regula... XM_024449586.1 81% 81% 0_1ins393;1450_1476del
22 human 4140 MARK3 microtubule affinity regula... XM_024449585.1 80.3% 80.3% 0_1ins393;1450_1494del
23 human 4140 MARK3 microtubule affinity regula... XM_017021301.2 79.4% 79.4% 0_1ins393;1450_1521del
24 human 4140 MARK3 microtubule affinity regula... XM_024449584.1 79.4% 79.4% 0_1ins393;1450_1521del
25 human 4140 MARK3 microtubule affinity regula... XM_017021308.2 78.8% 78.8% 0_1ins393;715_716ins48;1402_1428del
26 human 4140 MARK3 microtubule affinity regula... XM_024449590.1 78.3% 77.9% (many diffs)
27 human 4140 MARK3 microtubule affinity regula... XM_024449591.1 78.3% 77.9% (many diffs)
28 human 4140 MARK3 microtubule affinity regula... XM_024449588.1 78.2% 78.2% 0_1ins393;715_716ins48;1402_1446del
29 human 4140 MARK3 microtubule affinity regula... XM_024449589.1 76.7% 76.3% (many diffs)
30 human 4140 MARK3 microtubule affinity regula... XM_017021306.2 75.8% 75.4% (many diffs)
31 human 4140 MARK3 microtubule affinity regula... XM_017021307.2 75.8% 75.4% (many diffs)
32 mouse 17169 Mark3 MAP/microtubule affinity re... NM_022801.4 89.9% 96.9% (many diffs)
33 mouse 17169 Mark3 MAP/microtubule affinity re... NM_021516.4 88.1% 95% (many diffs)
34 mouse 17169 Mark3 MAP/microtubule affinity re... XM_006515518.3 87.8% 94.7% (many diffs)
35 mouse 17169 Mark3 MAP/microtubule affinity re... XM_006515515.3 87% 93.8% (many diffs)
36 mouse 17169 Mark3 MAP/microtubule affinity re... XM_006515517.3 85% 91.7% (many diffs)
37 mouse 17169 Mark3 MAP/microtubule affinity re... XM_006515514.3 83.8% 90.4% (many diffs)
38 mouse 17169 Mark3 MAP/microtubule affinity re... XM_006515512.3 82.2% 88.7% (many diffs)
39 mouse 17169 Mark3 MAP/microtubule affinity re... XM_006515511.3 81.3% 87.7% (many diffs)
40 mouse 17169 Mark3 MAP/microtubule affinity re... XM_006515513.3 79.4% 85.7% (many diffs)
41 mouse 17169 Mark3 MAP/microtubule affinity re... XM_006515519.1 64.1% 69.4% (many diffs)
42 mouse 17169 Mark3 MAP/microtubule affinity re... XM_006515522.3 52.4% 56.5% (many diffs)
43 mouse 17169 Mark3 MAP/microtubule affinity re... XM_011244006.1 40.9% 37.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2253
ORF length:
2187
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTC CACTAGGACC CCATTGCCAA CGGTGAATGA ACGAGACACT GAAAACCACA
121CGTCACATGG AGATGGGCGT CAAGAAGTTA CCTCTCGTAC CAGCCGCTCA GGAGCTCGGT
181GTAGAAACTC TATAGCCTCC TGTGCAGATG AACAACCTCA CATCGGAAAC TACAGACTGT
241TGAAAACAAT CGGCAAGGGG AATTTTGCAA AAGTAAAATT GGCAAGACAT ATCCTTACAG
301GCAGAGAGGT TGCAATAAAA ATAATTGACA AAACTCAGTT GAATCCAACA AGTCTACAAA
361AGCTCTTCAG AGAAGTAAGA ATAATGAAGA TTTTAAATCA TCCCAATATA GTGAAGTTAT
421TCGAAGTCAT TGAAACTGAA AAAACACTCT ACCTAATCAT GGAATATGCA AGTGGAGGTG
481AAGTATTTGA CTATTTGGTT GCACATGGCA GGATGAAGGA AAAAGAAGCA AGATCTAAAT
541TTAGACAGAT TGTGTCTGCA GTTCAATACT GCCATCAGAA ACGGATCGTA CATCGAGACC
601TCAAGGCTGA AAATCTATTG TTAGATGCCG ATATGAACAT TAAAATAGCA GATTTCGGTT
661TTAGCAATGA ATTTACTGTT GGCGGTAAAC TCGACACGTT TTGTGGCAGT CCTCCATACG
721CAGCACCTGA GCTCTTCCAG GGCAAGAAAT ATGACGGGCC AGAAGTGGAT GTGTGGAGTC
781TGGGGGTCAT TTTATACACA CTAGTCAGTG GCTCACTTCC CTTTGATGGG CAAAACCTAA
841AGGAACTGAG AGAGAGAGTA TTAAGAGGGA AATACAGAAT TCCCTTCTAC ATGTCTACAG
901ACTGTGAAAA CCTTCTCAAA CGTTTCCTGG TGCTAAATCC AATTAAACGC GGCACTCTAG
961AGCAAATCAT GAAGGACAGG TGGATCAATG CAGGGCATGA AGAAGATGAA CTCAAACCAT
1021TTGTTGAACC AGAGCTAGAC ATCTCAGACC AAAAAAGAAT AGATATTATG GTGGGAATGG
1081GATATTCACA AGAAGAAATT CAAGAATCTC TTAGTAAGAT GAAATACGAT GAAATCACAG
1141CTACATATTT GTTATTGGGG AGAAAATCTT CAGAGCTGGA TGCTAGTGAT TCCAGTTCTA
1201GCAGCAATCT TTCACTTGCT AAGGTTAGGC CGAGCAGTGA TCTCAACAAC AGTACTGGCC
1261AGTCTCCTCA CCACAAAGTG CAGAGAAGTG TTTCTTCAAG CCAAAAGCAA AGACGCTACA
1321GTGACCATGC TGGACCAGCT ATTCCTTCTG TTGTGGCGTA TCCGAAAAGG AGTCAGACCA
1381GCACTGCAGA TAGTGACCTC AAAGAAGATG GAATTTCCTC CCGGAAATCA AGTGGCAGTG
1441CTGTTGGAGG AAAGGGAATT GCTCCAGCCA GTCCCATGCT TGGGAATGCA AGTAATCCTA
1501ATAAGGCGGA TATTCCTGAA CGCAAGAAAA GCTCCACTGT CCCTAGTAGT AACACAGCAT
1561CTGGTGGAAT GACACGACGA AATACTTATG TTTGCAGTGA GAGAACTACA GCTGATAGAC
1621ACTCAGTGAT TCAGAATGGC AAAGAAAACA GCACTATTCC TGATCAGAGA ACTCCAGTTG
1681CTTCAACACA CAGTATCAGT AGTGCAGCCA CCCCAGATCG AATCCGCTTC CCAAGAGGCA
1741CTGCCAGTCG TAGCACTTTC CACGGCCAGC CCCGGGAACG GCGAACCGCA ACATATAATG
1801GCCCTCCTGC CTCTCCCAGC CTGTCCCATG AAGCCACACC ATTGTCCCAG ACTCGAAGCC
1861GAGGCTCCAC TAATCTCTTT AGTAAATTAA CTTCAAAACT CACAAGGAGT CGCAATGTAT
1921CTGCTGAGCA AAAAGATGAA AACAAAGAAG CAAAGCCTCG ATCCCTACGC TTCACCTGGA
1981GCATGAAAAC CACTAGTTCA ATGGATCCCG GGGACATGAT GCGGGAAATC CGCAAAGTGT
2041TGGACGCCAA TAACTGCGAC TATGAGCAGA GGGAGCGCTT CTTGCTCTTC TGCGTCCACG
2101GAGATGGGCA CGCGGAGAAC CTCGTGCAGT GGGAAATGGA AGTGTGCAAG CTGCCAAGAC
2161TGTCTCTGAA CGGGGTCCGG TTTAAGCGGA TATCGGGGAC ATCCATAGCC TTCAAAAATA
2221TTGCTTCCAA AATTGCCAAT GAGCTAAAGC TGTACCCAAC TTTCTTGTAC AAAGTtggca
2281ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)