Construct: ORF ccsbBroadEn_06644

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003847.1_s300c1, BRDN0000385520
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NME3 (4832)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4832 NME3 NME/NM23 nucleoside diphosp... NM_002513.2 99.8% 100% 282A>T
2 human 4832 NME3 NME/NM23 nucleoside diphosp... XM_005255332.4 97.4% 75% 282A>T;384_385insGTTGGCAA;495_496insTGAG
3 human 4832 NME3 NME/NM23 nucleoside diphosp... XM_011522503.2 96.1% 58.8% 177_187del;293A>T;395_396insGTTGGCAA
4 human 4832 NME3 NME/NM23 nucleoside diphosp... XM_011522504.2 76.4% 33.3% 177_187del;293A>T;408_409ins110
5 mouse 79059 Nme3 NME/NM23 nucleoside diphosp... NM_019730.2 82.8% 88.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
573
ORF length:
507
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAT CTGCCTGGTG CTGACCATCT TCGCTAACCT CTTCCCCGCG GCCTGCACCG
121GCGCACACGA ACGCACCTTC CTGGCCGTGA AGCCGGACGG CGTGCAGCGG CGGCTGGTGG
181GCGAGATTGT GCGGCGCTTC GAGAGGAAGG GCTTCAAGTT GGTGGCGCTG AAGCTGGTGC
241AGGCCTCCGA GGAGCTGCTG CGTGAGCACT ACGCCGAGCT GCGTGAACGC CCGTTCTACG
301GCCGCCTTGT CAAGTATATG GCCTCCGGGC CGGTGGTGGC CATGGTTTGG CAGGGGCTGG
361ACGTGGTGCG CACCTCGCGG GCGCTCATCG GAGCCACGAA CCCGGCCGAC GCCCCGCCCG
421GCACCATCCG CGGGGATTTC TGCATCGAGG TTGGCAAGAA CCTGATTCAC GGCAGCGACT
481CGGTGGAGAG TGCCCGCCGC GAGATCGCTC TCTGGTTCCG CGCAGACGAG CTCCTCTGCT
541GGGAGGACAG CGCTGGGCAC TGGCTGTATG AGTACCCAAC TTTCTTGTAC AAAGTtggca
601ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)