Construct: ORF ccsbBroadEn_06732

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015711.1_s300c1, BRDN0000392778
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PKNOX1 (5316)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5316 PKNOX1 PBX/knotted 1 homeobox 1 NM_004571.5 99.9% 100% 342T>C
2 human 5316 PKNOX1 PBX/knotted 1 homeobox 1 NM_001320694.2 99.6% 99.7% 342T>C;519_520insCAG
3 human 5316 PKNOX1 PBX/knotted 1 homeobox 1 NM_001286258.1 73.1% 73.1% 0_1ins351
4 mouse 18771 Pknox1 Pbx/knotted 1 homeobox NM_016670.3 87.5% 96.1% (many diffs)
5 mouse 18771 Pknox1 Pbx/knotted 1 homeobox XM_006523854.2 87.5% 96.1% (many diffs)
6 mouse 18771 Pknox1 Pbx/knotted 1 homeobox XM_006523855.2 87.5% 96.1% (many diffs)
7 mouse 18771 Pknox1 Pbx/knotted 1 homeobox XM_006523856.3 87.3% 95.8% (many diffs)
8 mouse 18771 Pknox1 Pbx/knotted 1 homeobox XM_006523857.3 87.3% 95.8% (many diffs)
9 mouse 18771 Pknox1 Pbx/knotted 1 homeobox XM_006523858.1 87.1% 95.6% (many diffs)
10 mouse 18771 Pknox1 Pbx/knotted 1 homeobox NR_027493.1 25.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1374
ORF length:
1308
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAT GGCTACACAG ACATTAAGTA TAGACAGCTA TCAAGATGGG CAACAGATGC
121AAGTAGTAAC AGAGTTAAAG ACAGAACAAG ATCCAAACTG CTCTGAACCC GATGCAGAAG
181GAGTGAGCCC TCCCCCTGTG GAGTCTCAGA CCCCGATGGA TGTGGACAAG CAGGCCATTT
241ATAGGCATCC ACTATTTCCA TTATTAGCTT TGTTGTTTGA AAAATGTGAA CAATCTACAC
301AGGGCTCTGA AGGCACAACT TCTGCCAGTT TTGATGTAGA CATCGAAAAT TTTGTAAGAA
361AGCAAGAGAA GGAAGGGAAA CCTTTCTTTT GTGAAGATCC AGAAACCGAT AATTTAATGG
421TAAAAGCAAT CCAGGTTTTG CGCATTCATC TTCTTGAGCT GGAAAAGGTT AACGAACTCT
481GCAAAGATTT CTGCAGTCGA TACATTGCTT GTCTGAAAAC AAAAATGAAC AGTGAAACTC
541TGTTGAGTGG AGAGCCTGGA AGCCCGTACT CACCAGTGCA GTCCCAGCAG ATTCAAAGTG
601CCATCACAGG CACCATCAGC CCTCAGGGAA TTGTGGTGCC GGCGTCCGCG CTGCAGCAGG
661GAAACGTAGC CATGGCGACG GTGGCAGGTG GCACAGTGTA TCAGCCTGTC ACGGTCGTCA
721CTCCCCAAGG CCAAGTGGTC ACACAGACAT TGTCGCCTGG GACAATTAGG ATCCAGAACT
781CCCAGCTTCA GTTACAGTTA AACCAAGATC TCAGCATCTT GCATCAAGAT GATGGTTCAT
841CTAAGAACAA GAGGGGCGTC CTGCCAAAGC ATGCCACGAA CGTGATGCGG TCCTGGCTCT
901TCCAGCACAT CGGGCATCCC TACCCAACAG AGGATGAGAA AAAACAGATT GCTGCTCAGA
961CAAATTTGAC ACTACTCCAA GTCAACAACT GGTTCATCAA TGCCAGAAGA CGAATTCTTC
1021AGCCAATGTT GGATTCAAGT TGTTCAGAGA CCCCCAAAAC AAAGAAAAAA ACTGCTCAGA
1081ACCGGCCAGT TCAGAGGTTT TGGCCTGATT CTATTGCATC AGGAGTCGCA CAGCCACCGC
1141CGAGCGAGCT CACCATGTCG GAAGGAGCTG TTGTCACCAT CACCACGCCC GTGAACATGA
1201ACGTGGACAG CCTTCAGTCT CTGTCCTCGG ACGGGGCCAC CCTGGCGGTG CAGCAGGTCA
1261TGATGGCAGG GCAGAGCGAG GACGAGTCTG TGGACAGCAC AGAGGAGGAT GCGGGTGCCC
1321TGGCCCCTGC CCACATCAGC GGGCTGGTCT TGGAGAACAG TGACTCCCTG CAGTACCCAA
1381CTTTCTTGTA CAAAGTtggc attataagaa agcattgctt atcaatttgt tgcaacgaac

Download FASTA (ORF) (Full)