Construct: ORF ccsbBroadEn_06741

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012548.1_s300c1, BRDN0000392441
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PLSCR1 (5359)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5359 PLSCR1 phospholipid scramblase 1 NM_021105.3 99.8% 99.6% 947G>T
2 human 5359 PLSCR1 phospholipid scramblase 1 XM_011512904.1 99.8% 99.6% 947G>T
3 human 5359 PLSCR1 phospholipid scramblase 1 XM_017006629.1 99.8% 99.6% 947G>T
4 human 5359 PLSCR1 phospholipid scramblase 1 NM_001363872.1 97.6% 97.4% 0_1ins21;926G>T
5 human 5359 PLSCR1 phospholipid scramblase 1 XM_017006628.1 94.9% 91.4% (many diffs)
6 human 5359 PLSCR1 phospholipid scramblase 1 NM_001363874.1 74.4% 66.1% 0_1ins25;67_68ins218;704G>T
7 human 5359 PLSCR1 phospholipid scramblase 1 XM_005247538.3 74.4% 66.1% 0_1ins25;67_68ins218;704G>T
8 human 5359 PLSCR1 phospholipid scramblase 1 XM_011512906.2 74.4% 66.1% 0_1ins25;67_68ins218;704G>T
9 human 57047 PLSCR2 phospholipid scramblase 2 NM_001199978.1 73.3% 66.4% (many diffs)
10 human 57047 PLSCR2 phospholipid scramblase 2 XM_024453670.1 73.3% 66.4% (many diffs)
11 human 57047 PLSCR2 phospholipid scramblase 2 XM_024453671.1 73.3% 66.4% (many diffs)
12 human 57047 PLSCR2 phospholipid scramblase 2 XM_024453672.1 73.3% 66.4% (many diffs)
13 human 57047 PLSCR2 phospholipid scramblase 2 XM_024453673.1 73.3% 66.4% (many diffs)
14 human 57047 PLSCR2 phospholipid scramblase 2 XM_024453674.1 73.3% 66.4% (many diffs)
15 human 57047 PLSCR2 phospholipid scramblase 2 NM_001199979.1 72.4% 62.9% (many diffs)
16 human 57047 PLSCR2 phospholipid scramblase 2 XM_011513022.3 71.5% 61.7% (many diffs)
17 human 57047 PLSCR2 phospholipid scramblase 2 XM_011513023.3 71.5% 61.7% (many diffs)
18 human 57047 PLSCR2 phospholipid scramblase 2 XM_017006903.2 71.5% 61.7% (many diffs)
19 human 57047 PLSCR2 phospholipid scramblase 2 XM_017006904.2 71.5% 61.7% (many diffs)
20 human 57047 PLSCR2 phospholipid scramblase 2 NM_020359.2 62.9% 59.4% (many diffs)
21 human 57047 PLSCR2 phospholipid scramblase 2 XM_017006910.2 62.9% 59.4% (many diffs)
22 human 57047 PLSCR2 phospholipid scramblase 2 XM_017006911.1 62.9% 59.4% (many diffs)
23 human 57047 PLSCR2 phospholipid scramblase 2 XM_024453675.1 62.9% 59.4% (many diffs)
24 human 57047 PLSCR2 phospholipid scramblase 2 XM_024453676.1 62.9% 59.4% (many diffs)
25 human 57047 PLSCR2 phospholipid scramblase 2 XM_024453677.1 61.8% 52.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1020
ORF length:
954
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA CAAACAAAAC TCACAGATGA ATGCTTCTCA CCCGGAAACA AACTTGCCAG
121TTGGGTATCC TCCTCAGTAT CCACCGACAG CATTCCAAGG ACCTCCAGGA TATAGTGGCT
181ACCCTGGGCC CCAGGTCAGC TACCCACCCC CACCAGCCGG CCATTCAGGT CCTGGCCCAG
241CTGGCTTTCC TGTCCCAAAT CAGCCAGTGT ATAATCAGCC AGTATATAAT CAGCCAGTTG
301GAGCTGCAGG GGTACCATGG ATGCCAGCGC CACAGCCTCC ATTAAACTGT CCACCTGGAT
361TAGAATATTT AAGTCAGATA GATCAGATAC TGATTCATCA GCAAATTGAA CTTCTGGAAG
421TTTTAACAGG TTTTGAAACT AATAACAAAT ATGAAATTAA GAACAGCTTT GGACAGAGGG
481TTTACTTTGC AGCGGAAGAT ACTGATTGCT GTACCCGAAA TTGCTGTGGG CCATCTAGAC
541CTTTTACCTT GAGGATTATT GATAATATGG GTCAAGAAGT CATAACTCTG GAGAGACCAC
601TAAGATGTAG CAGCTGTTGT TGTCCCTGCT GCCTTCAGGA GATAGAAATC CAAGCTCCTC
661CTGGTGTACC AATAGGTTAT GTTATTCAGA CTTGGCACCC ATGTCTACCA AAGTTTACAA
721TTCAAAATGA GAAAAGAGAG GATGTACTAA AAATAAGTGG TCCATGTGTT GTGTGCAGCT
781GTTGTGGAGA TGTTGATTTT GAGATTAAAT CTCTTGATGA ACAGTGTGTG GTTGGCAAAA
841TTTCCAAGCA CTGGACTGGA ATTTTGAGAG AGGCATTTAC AGACGCTGAT AACTTTGGAA
901TCCAGTTCCC TTTAGACCTT GATGTTAAAA TGAAAGCTGT AATGATTGGT GCCTGTTTCC
961TCATTGACTT CATGTTTTTT GAAAGCACTG GCAGCCAGGA ACAAAAATCA GTAGTGTGGT
1021ACCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca
1081acgaac

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