Construct: ORF ccsbBroadEn_06857
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016915.1_s300c1, BRDN0000389428
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RFC4 (5984)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5984 | RFC4 | replication factor C subunit 4 | NM_002916.4 | 99.9% | 100% | 798C>T |
| 2 | human | 5984 | RFC4 | replication factor C subunit 4 | NM_181573.2 | 99.9% | 100% | 798C>T |
| 3 | mouse | 106344 | Rfc4 | replication factor C (activ... | NM_145480.1 | 86.9% | 90.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1155
- ORF length:
- 1089
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGCA AGCATTTCTT AAAGGTACAT CCATCAGTAC TAAACCCCCG CTGACCAAGG 121 ATCGAGGAGT AGCTGCCAGT GCGGGAAGTA GCGGAGAGAA CAAGAAAGCC AAACCCGTTC 181 CCTGGGTGGA AAAATATCGC CCAAAATGTG TGGATGAAGT TGCTTTCCAG GAAGAAGTGG 241 TTGCAGTGCT GAAAAAATCT TTAGAAGGAG CAGATCTTCC TAATCTCTTG TTTTACGGAC 301 CACCTGGAAC TGGAAAAACA TCCACTATTT TGGCAGCAGC TAGAGAACTC TTTGGGCCTG 361 AACTTTTCCG ATTAAGAGTT CTTGAGTTAA ATGCATCTGA TGAACGTGGA ATACAAGTAG 421 TTCGAGAGAA AGTGAAAAAT TTTGCTCAAT TAACTGTGTC AGGAAGTCGC TCAGATGGGA 481 AGCCGTGTCC GCCTTTTAAG ATTGTGATTC TGGATGAAGC AGATTCTATG ACCTCAGCTG 541 CTCAGGCAGC TTTAAGACGT ACCATGGAGA AGGAGTCGAA AACCACCCGA TTCTGTCTTA 601 TCTGTAACTA TGTCAGTCGA ATAATTGAAC CCCTGACCTC TAGATGTTCA AAATTCCGCT 661 TCAAGCCTCT GTCAGATAAA ATTCAACAGC AGCGATTACT AGACATTGCC AAGAAGGAAA 721 ATGTCAAAAT TAGTGATGAG GGAATAGCTT ATCTTGTTAA AGTGTCAGAA GGAGACTTAA 781 GAAAAGCCAT TACATTTCTT CAAAGCGCTA CTCGATTAAC AGGTGGAAAG GAGATCACAG 841 AGAAAGTGAT TACAGACATT GCTGGGGTAA TACCAGCTGA GAAAATTGAT GGAGTATTTG 901 CTGCCTGTCA GAGTGGCTCT TTTGACAAAC TAGAAGCTGT GGTCAAGGAT TTAATAGATG 961 AGGGTCATGC AGCAACTCAG CTCGTCAATC AACTCCATGA TGTGGTTGTA GAAAATAACT 1021 TATCTGATAA ACAGAAGTCT ATTATCACAG AAAAACTTGC CGAAGTTGAC AAATGCCTAG 1081 CAGATGGTGC TGATGAACAT TTGCAACTCA TCAGCCTTTG TGCAACTGTG ATGCAGCAGT 1141 TATCTCAGAA TTGTTACCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct 1201 tatcaatttg ttgcaacgaa c