Construct: ORF ccsbBroadEn_07022

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016983.1_s300c1, BRDN0000391838
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
VAMP1 (6843)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6843 VAMP1 vesicle associated membrane... NM_199245.3 99.7% 100% 252C>A
2 human 6843 VAMP1 vesicle associated membrane... NM_001297438.2 98% 96.5% (many diffs)
3 human 6843 VAMP1 vesicle associated membrane... NM_016830.4 96% 96.5% 252C>A;341_342insTAAGTATC;344_348delGGGAC
4 human 6843 VAMP1 vesicle associated membrane... NM_014231.5 95.2% 95.7% (many diffs)
5 human 6843 VAMP1 vesicle associated membrane... NR_123717.2 28.6% (many diffs)
6 mouse 22317 Vamp1 vesicle-associated membrane... NM_001080557.1 93.1% 98.2% (many diffs)
7 mouse 22317 Vamp1 vesicle-associated membrane... NM_009496.3 88.8% 94% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
417
ORF length:
351
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTC TGCTCCAGCT CAGCCACCTG CTGAAGGGAC AGAAGGGACT GCCCCAGGTG
121GGGGTCCCCC TGGCCCTCCT CCTAACATGA CCAGTAACAG ACGACTACAG CAAACCCAGG
181CACAAGTGGA GGAGGTGGTG GACATCATAC GTGTGAACGT GGACAAGGTC CTGGAGAGGG
241ACCAGAAGCT GTCAGAGCTG GATGACCGAG CTGATGCCTT GCAGGCAGGA GCATCACAAT
301TTGAGAGCAG TGCTGCAAAG CTAAAGAGGA AGTATTGGTG GAAAAACTGC AAGATGATGA
361TCATGCTGGG AGCCATCTGT GCCATCATCG TGGTAGTTAT TGTAAGTAAG TATCGCTGCC
421CAACTTTCTT GTACAAAGTt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
481aac

Download FASTA (ORF) (Full)