Construct: ORF ccsbBroadEn_07023
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018069.1_s300c1, BRDN0000388515
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYK (6850)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6850 | SYK | spleen associated tyrosine ... | NM_001174167.3 | 99.7% | 100% | (many diffs) |
| 2 | human | 6850 | SYK | spleen associated tyrosine ... | NM_003177.7 | 99.7% | 100% | (many diffs) |
| 3 | human | 6850 | SYK | spleen associated tyrosine ... | XM_005252147.4 | 99.7% | 100% | (many diffs) |
| 4 | human | 6850 | SYK | spleen associated tyrosine ... | XM_011518946.3 | 99.7% | 100% | (many diffs) |
| 5 | human | 6850 | SYK | spleen associated tyrosine ... | NM_001135052.4 | 96.1% | 96.3% | (many diffs) |
| 6 | human | 6850 | SYK | spleen associated tyrosine ... | NM_001174168.3 | 96.1% | 96.3% | (many diffs) |
| 7 | human | 6850 | SYK | spleen associated tyrosine ... | XR_001746370.2 | 37.5% | (many diffs) | |
| 8 | mouse | 20963 | Syk | spleen tyrosine kinase | NM_001198977.1 | 84.6% | 92.1% | (many diffs) |
| 9 | mouse | 20963 | Syk | spleen tyrosine kinase | NM_011518.2 | 84.6% | 92.1% | (many diffs) |
| 10 | mouse | 20963 | Syk | spleen tyrosine kinase | XM_006516895.4 | 84.6% | 92.1% | (many diffs) |
| 11 | mouse | 20963 | Syk | spleen tyrosine kinase | XM_006516897.4 | 81.6% | 88.8% | (many diffs) |
| 12 | mouse | 20963 | Syk | spleen tyrosine kinase | XM_017315471.2 | 47.5% | 49.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1971
- ORF length:
- 1905
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAGAAAG 61 TTGGCATGGC CAGCAGCGGC ATGGCTGACA GCGCCAACCA CCTGCCCTTC TTTTTCGGCA 121 ACATCACCCG GGAGGAGGCA GAAGATTACC TGGTCCAGGG GGGCATGAGT GATGGGCTTT 181 ATTTGCTGCG CCAGAGCCGC AACTACCTGG GTGGCTTCGC CCTGTCCGTG GCCCACGGGA 241 GGAAGGCACA CCACTACACC ATCGAGCGGG AGCTGAATGG CACCTACGCC ATCGCCGGTG 301 GCAGGACCCA TGCCAGCCCC GCCGACCTCT GCCACTACCA CTCCCAGGAG TCTGATGGCC 361 TGGTCTGCCT CCTCAAGAAG CCCTTCAACC GGCCCCAAGG GGTGCAGCCC AAGACTGGGC 421 CCTTTGAGGA TTTGAAGGAA AACCTCATCA GGGAATATGT GAAGCAGACA TGGAACCTGC 481 AGGGTCAGGC TCTGGAGCAG GCCATCATCA GTCAGAAGCC TCAGCTGGAG AAGCTGATCG 541 CTACCACAGC CCATGAAAAA ATGCCTTGGT TCCATGGAAA AATCTCTCGG GAAGAATCTG 601 AGCAAATTGT CCTGATAGGA TCAAAGACAA ATGGAAAGTT CCTGATCCGA GCCAGAGACA 661 ACAACGGCTC CTACGCCCTG TGCCTGCTGC ACGAAGGGAA GGTGCTGCAC TATCGCATCG 721 ACAAAGACAA GACAGGGAAG CTCTCCATCC CCGAGGGAAA GAAGTTCGAC ACGCTCTGGC 781 AGCTAGTCGA GCATTATTCT TATAAAGCAG ATGGTTTGTT AAGAGTTCTT ACTGTCCCAT 841 GTCAAAAAAT CGGCACACAG GGAAATGTTA ATTTTGGAGG CCGTCCACAA CTTCCAGGTT 901 CCCATCCTGC GACTTGGTCA GCGGGTGGAA TAATCTCAAG AATCAAATCA TACTCCTTCC 961 CAAAGCCTGG CCACAGAAAG TCCTCCCCTG CCCAAGGGAA CCGGCAAGAG AGTACTGTGT 1021 CATTCAATCC GTATGAGCCA GAACTTGCAC CCTGGGCTGC AGACAAAGGC CCCCAGAGAG 1081 AAGCCCTACC CATGGACACA GAGGTGTACG AGAGCCCCTA CGCGGACCCT GAGGAGATCA 1141 GGCCCAAGGA GGTTTACCTG GACCGAAAGC TGCTGACGCT GGAAGACAAA GAACTGGGCT 1201 CTGGTAATTT TGGAACTGTG AAAAAGGGCT ACTACCAAAT GAAAAAAGTT GTGAAAACCG 1261 TGGCTGTGAA AATACTGAAA AACGAGGCCA ATGACCCCGC TCTTAAAGAT GAGTTATTAG 1321 CAGAAGCAAA TGTCATGCAG CAGCTGGACA ACCCGTACAT CGTGCGCATG ATCGGGATAT 1381 GCGAGGCCGA GTCCTGGATG CTAGTTATGG AGATGGCAGA ACTTGGTCCC CTCAATAAGT 1441 ATTTGCAGCA GAACAGACAT GTCAAGGATA AGAACATCAT AGAACTGGTT CATCAGGTTT 1501 CCATGGGCAT GAAGTACTTG GAGGAGAGCA ATTTTGTGCA CAGAGATCTG GCTGCAAGAA 1561 ATGTGTTGCT AGTTACCCAA CATTATGCCA AGATCAGTGA TTTCGGACTC TCCAAAGCAC 1621 TGCGTGCTGA TGAAAACTAC TACAAGGCCC AGACCCATGG AAAGTGGCCT GTCAAGTGGT 1681 ACGCTCCGGA ATGCATCAAC TACTACAAGT TCTCCAGCAA AAGCGATGTC TGGAGCTTTG 1741 GAGTGTTGAT GTGGGAAGCA TTCTCCTATG GGCAGAAGCC ATATCGAGGG ATGAAAGGAA 1801 GTGAAGTCAC CGCTATGTTA GAGAAAGGAG AGCGGATGGG GTGCCCTGCA GGGTGTCCAA 1861 GAGAGATGTA CGATCTCATG AATCTGTGCT GGACATACGA TGTGGAAAAC AGGCCCGGAT 1921 TCGCAGCAGT GGAACTGCGG CTGCGCAATT ACTACTATGA CGTGGTGAAC TACCCAACTT 1981 TATTGTACAA AGTtggcatt ataagaaagc attgcttatc aatttgttgc aacgaac