Construct: ORF ccsbBroadEn_07079
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004158.1_s300c1, BRDN0000388939
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSPAN4 (7106)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7106 | TSPAN4 | tetraspanin 4 | NM_001025234.1 | 99.8% | 100% | 166T>C |
| 2 | human | 7106 | TSPAN4 | tetraspanin 4 | NM_001025235.1 | 99.8% | 100% | 166T>C |
| 3 | human | 7106 | TSPAN4 | tetraspanin 4 | NM_001025236.1 | 99.8% | 100% | 166T>C |
| 4 | human | 7106 | TSPAN4 | tetraspanin 4 | NM_001025237.1 | 99.8% | 100% | 166T>C |
| 5 | human | 7106 | TSPAN4 | tetraspanin 4 | NM_001025238.2 | 99.8% | 100% | 166T>C |
| 6 | human | 7106 | TSPAN4 | tetraspanin 4 | NM_003271.5 | 99.8% | 100% | 166T>C |
| 7 | human | 7106 | TSPAN4 | tetraspanin 4 | XM_005253102.1 | 99.8% | 100% | 166T>C |
| 8 | human | 7106 | TSPAN4 | tetraspanin 4 | XM_011520336.1 | 84.8% | 80.5% | (many diffs) |
| 9 | human | 7106 | TSPAN4 | tetraspanin 4 | NM_001025239.1 | 73.1% | 73.1% | 0_1ins192 |
| 10 | human | 7106 | TSPAN4 | tetraspanin 4 | XM_005253104.1 | 73.1% | 73.1% | 63_64ins192 |
| 11 | human | 7106 | TSPAN4 | tetraspanin 4 | XM_011520337.2 | 73.1% | 73.1% | 0_1ins192 |
| 12 | human | 7106 | TSPAN4 | tetraspanin 4 | XM_011520338.1 | 73.1% | 73.1% | 0_1ins192 |
| 13 | human | 7106 | TSPAN4 | tetraspanin 4 | XM_011520339.2 | 73.1% | 73.1% | 63_64ins192 |
| 14 | human | 7106 | TSPAN4 | tetraspanin 4 | XM_011520340.2 | 73.1% | 73.1% | 0_1ins192 |
| 15 | human | 7106 | TSPAN4 | tetraspanin 4 | XM_011520341.2 | 73.1% | 73.1% | 0_1ins192 |
| 16 | mouse | 64540 | Tspan4 | tetraspanin 4 | NM_001252588.1 | 87.3% | 93.6% | (many diffs) |
| 17 | mouse | 64540 | Tspan4 | tetraspanin 4 | NM_053082.3 | 87.3% | 93.6% | (many diffs) |
| 18 | mouse | 64540 | Tspan4 | tetraspanin 4 | XM_006536224.3 | 76.7% | 82.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 780
- ORF length:
- 714
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC GCGCGCCTGC CTCCAGGCCG TCAAGTACCT CATGTTCGCC TTCAACCTGC 121 TCTTCTGGCT GGGAGGCTGT GGCGTGCTGG GTGTCGGCAT CTGGCTGGCC GCCACACAGG 181 GGAGCTTCGC CACGCTGTCC TCTTCCTTCC CGTCCCTGTC GGCTGCCAAC CTGCTCATCA 241 TCACCGGCGC CTTTGTCATG GCCATCGGCT TCGTGGGCTG CCTGGGTGCC ATCAAGGAGA 301 ACAAGTGCCT CCTGCTCACT TTCTTCCTGC TGCTGCTGCT GGTGTTCCTG CTGGAGGCCA 361 CCATCGCCAT CCTCTTCTTC GCCTACACGG ACAAGATTGA CAGGTATGCC CAGCAAGACC 421 TGAAGAAAGG CTTGCACCTG TACGGCACGC AGGGCAACGT GGGCCTCACC AACGCCTGGA 481 GCATCATCCA GACCGACTTC CGCTGCTGTG GCGTCTCCAA CTACACTGAC TGGTTCGAGG 541 TGTACAACGC CACGCGGGTA CCTGACTCCT GCTGCTTGGA GTTCAGTGAG AGCTGTGGGC 601 TGCACGCCCC CGGCACCTGG TGGAAGGCGC CGTGCTACGA GACGGTGAAG GTGTGGCTTC 661 AGGAGAACCT GCTGGCTGTG GGCATCTTTG GGCTGTGCAC GGCGCTGGTG CAGATCCTGG 721 GCCTGACCTT CGCCATGACC ATGTACTGCC AAGTGGTCAA GGCAGACACC TACTGCGCGT 781 ACCCAACTTT CTTGTACAAA GTtggcatta taagaaagca ttgcttatca atttgttgca 841 acgaac