Construct: ORF ccsbBroadEn_07207

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013339.1_s300c1, BRDN0000397925
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GDF5 (8200)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8200 GDF5 growth differentiation fact... NM_000557.5 99.8% 99.8% 826G>T;1017A>G;1401G>T
2 human 8200 GDF5 growth differentiation fact... NM_001319138.2 99.8% 99.8% 826G>T;1017A>G;1401G>T
3 mouse 14563 Gdf5 growth differentiation fact... NM_008109.3 89.9% 91.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1569
ORF length:
1503
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAG ACTCCCCAAA CTCCTCACTT TCTTGCTTTG GTACCTGGCT TGGCTGGACC
121TGGAATTCAT CTGCACTGTG TTGGGTGCCC CTGACTTGGG CCAGAGACCC CAGGGGACCA
181GGCCAGGATT GGCCAAAGCA GAGGCCAAGG AGAGGCCCCC CCTGGCCCGG AACGTCTTCA
241GGCCAGGGGG TCACAGCTAT GGTGGGGGGG CCACCAATGC CAATGCCAGG GCAAAGGGAG
301GCACCGGGCA GACAGGAGGC CTGACACAGC CCAAGAAGGA TGAACCCAAA AAGCTGCCCC
361CCAGACCGGG CGGCCCTGAA CCCAAGCCAG GACACCCTCC CCAAACAAGG CAGGCTACAG
421CCCGGACTGT GACCCCAAAA GGACAGCTTC CCGGAGGCAA GGCACCCCCA AAAGCAGGAT
481CTGTCCCCAG CTCCTTCCTG CTGAAGAAGG CCAGGGAGCC CGGGCCCCCA CGAGAGCCCA
541AGGAGCCGTT TCGCCCACCC CCCATCACAC CCCACGAGTA CATGCTCTCG CTGTACAGGA
601CGCTGTCCGA TGCTGACAGA AAGGGAGGCA ACAGCAGCGT GAAGTTGGAG GCTGGCCTGG
661CCAACACCAT CACCAGCTTT ATTGACAAAG GGCAAGATGA CCGAGGTCCC GTGGTCAGGA
721AGCAGAGGTA CGTGTTTGAC ATTAGTGCCC TGGAGAAGGA TGGGCTGCTG GGGGCCGAGC
781TGCGGATCTT GCGGAAGAAG CCCTCGGACA CGGCCAAGCC AGCGGCCCCC GGAGGCGGGC
841GGGCTGCCCA GCTGAAGCTG TCCAGCTGCC CCAGCGGCCG GCAGCCGGCC TCCTTGCTGG
901ATGTGCGCTC CGTGCCAGGC CTGGACGGAT CTGGCTGGGA GGTGTTCGAC ATCTGGAAGC
961TCTTCCGAAA CTTTAAGAAC TCGGCCCAGC TGTGCCTGGA GCTGGAGGCC TGGGAACGGG
1021GCAGGGCCGT GGACCTCCGT GGCCTGGGCT TCGACCGCGC CGCCCGGCAG GTCCACGAGA
1081AGGCCCTGTT CCTGGTGTTT GGCCGCACCA AGAAACGGGA CCTGTTCTTT AATGAGATTA
1141AGGCCCGCTC TGGCCAGGAC GATAAGACCG TGTATGAGTA CCTGTTCAGC CAGCGGCGAA
1201AACGGCGGGC CCCACTGGCC ACTCGCCAGG GCAAGCGACC CAGCAAGAAC CTTAAGGCTC
1261GCTGCAGTCG GAAGGCACTG CATGTCAACT TCAAGGACAT GGGCTGGGAC GACTGGATCA
1321TCGCACCCCT TGAGTACGAG GCTTTCCACT GCGAGGGGCT GTGCGAGTTC CCATTGCGCT
1381CCCACCTGGA GCCCACGAAT CATGCAGTCA TCCAGACCCT GATGAACTCC ATGGACCCCG
1441AGTCCACACC ACCCACCTGC TGTGTTCCCA CGCGGCTGAG TCCCATCAGC ATCCTCTTCA
1501TTGACTCTGC CAACAACGTG GTGTATAAGC AGTATGAGGA CATGGTCGTG GAGTCGTGTG
1561GCTGCAGGTA CCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa
1621tttgttgcaa cgaac

Download FASTA (ORF) (Full)