Construct: ORF ccsbBroadEn_07239

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008928.1_s300c1, BRDN0000457245
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NSMAF (8439)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8439 NSMAF neutral sphingomyelinase ac... NM_003580.4 99.9% 100% 1428C>T
2 human 8439 NSMAF neutral sphingomyelinase ac... NM_001144772.1 95.9% 94.9% (many diffs)
3 mouse 18201 Nsmaf neutral sphingomyelinase (N... NM_010945.2 87.3% 91.7% (many diffs)
4 mouse 18201 Nsmaf neutral sphingomyelinase (N... XM_006537664.2 86.7% 91% (many diffs)
5 mouse 18201 Nsmaf neutral sphingomyelinase (N... XM_006537663.2 84.6% 88.9% (many diffs)
6 mouse 18201 Nsmaf neutral sphingomyelinase (N... XM_006537662.3 84.1% 88.2% (many diffs)
7 mouse 18201 Nsmaf neutral sphingomyelinase (N... XM_011249943.2 71.4% 75% (many diffs)
8 mouse 18201 Nsmaf neutral sphingomyelinase (N... XM_011249944.2 71% 75% (many diffs)
9 mouse 18201 Nsmaf neutral sphingomyelinase (N... XR_390314.3 59.9% (many diffs)
10 mouse 18201 Nsmaf neutral sphingomyelinase (N... XR_001784107.1 51.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2817
ORF length:
2751
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC GTTTATCCGG AAGAAGCAGC AGGAGCAGCA GCTGCAGCTC TACTCCAAGG
121AGAGATTTTC CTTGCTGCTG CTTAACTTGG AGGAGTACTA CTTTGAACAG CATAGAGCCA
181ATCACATTTT GCACAAGGGC AGTCACCATG AAAGGAAAAT CAGAGGCTCC TTAAAAATAT
241GTTCAAAATC GGTGATTTTT GAACCAGATT CAATATCCCA GCCCATCATC AAGATTCCTT
301TGAGAGACTG TATAAAAATA GGAAAGCATG GAGAAAATGG AGCCAATAGA CACTTCACAA
361AGGCAAAATC TGGGGGTATT TCACTCATTT TCAGTCAGGT ATATTTCATT AAAGAACATA
421ATGTTGTTGC ACCATATAAA ATAGAAAGGG GCAAAATGGA ATATGTTTTT GAATTGGATG
481TTCCCGGGAA AGTGGAAGAT GTTGTGGAGA CGTTGCTTCA GCTTCACAGA GCATCCTGCC
541TTGACAAATT GGGTGACCAA ACCGCCATGA TAACAGCTAT TTTGCAGTCT CGTTTAGCTA
601GAACATCATT TGACAAAAAC AGGTTCCAAA ACATTTCTGA AAAGCTGCAC ATGGAATGCA
661AAGCAGAAAT GGTGACGCCT CTGGTGACTA ATCCTGGACA CGTGTGCATC ACGGACACAA
721ACCTGTATTT TCAGCCCCTC AACGGCTACC CGAAACCTGT GGTCCAGATA ACACTCCAAG
781ATGTCCGCCG CATCTACAAA AGGAGGCACG GCCTCATGCC TCTGGGCTTG GAAGTATTTT
841GCACAGAAGA TGATCTGTGT TCCGACATCT ACCTAAAGTT CTATGAACCT CAAGATAGAG
901ATGATCTCTA TTTTTACATT GCCACATACC TAGAGCACCA TGTGGCGGAG CACACTGCTG
961AGAGCTACAT GCTGCAGTGG CAGCGTGGAC ACCTTTCCAA CTATCAGTAC CTCCTTCACC
1021TCAACAACCT GGCCGACCGC AGCTGCAACG ACCTCTCCCA GTACCCTGTG TTTCCATGGA
1081TAATACATGA TTATTCCAGC TCAGAACTAG ATTTGTCAAA TCCAGGAACC TTCCGGGATC
1141TCAGTAAGCC AGTAGGGGCC CTAAATAAGG AACGGCTGGA GAGACTACTG ACACGCTACC
1201AGGAAATGCC TGAACCAAAG TTCATGTATG GGAGTCACTA CTCTTCCCCG GGTTATGTAC
1261TTTTTTATCT TGTTAGGATT GCACCAGAGT ATATGCTGTG CCTGCAGAAT GGAAGATTTG
1321ATAATGCAGA TAGAATGTTC AACAGTATTG CAGAAACTTG GAAAAACTGT CTGGATGGTG
1381CAACGGATTT TAAAGAGTTA ATTCCAGAAT TCTATGGTGA TGATGTGAGC TTTCTAGTCA
1441ATAGCCTGAA GTTGGATTTG GGAAAGAGAC AAGGAGGACA GATGGTTGAC GATGTGGAGC
1501TTCCCCCTTG GGCTTCCAGT CCCGAGGACT TTCTCCAGAA GAGCAAAGAT GCATTGGAAA
1561GCAATTATGT GTCTGAACAC CTTCACGAGT GGATTGATCT AATATTTGGC TACAAACAAA
1621AAGGGAGTGA TGCAGTTGGG GCCCATAATG TATTTCATCC CCTGACCTAT GAAGGAGGTG
1681TAGACTTGAA CAGCATCCAG GATCCTGATG AGAAGGTAGC CATGCTTACG CAAATCTTGG
1741AATTTGGGCA GACACCAAAA CAACTATTTG TGACACCACA TCCTCGAAGG ATCACCCCAA
1801AGTTTAAAAG TTTGTCCCAG ACCTCCAGTT ATAATGCTTC TATGGCAGAT TCCCCAGGTG
1861AAGAGTCTTT TGAAGACCTG ACCGAAGAAA GCAAAACACT GGCCTGGAAT AACATCACCA
1921AACTGCAGTT ACACGAGCAC TATAAAATCC ACAAAGAAGC AGTTACTGGA ATCACGGTCT
1981CTCGCAATGG ATCTTCAGTA TTCACAACAT CCCAAGATTC CACCTTGAAG ATGTTTTCTA
2041AAGAATCAAA AATGCTACAA AGAAGTATAT CATTTTCAAA TATGGCTTTA TCGTCTTGTT
2101TACTTTTACC AGGAGATGCC ACTGTCATAA CTTCTTCATG GGATAATAAT GTCTATTTTT
2161ATTCCATAGC ATTTGGAAGA CGCCAGGACA CGTTAATGGG ACATGATGAT GCTGTTAGTA
2221AGATCTGTTG GCATGACAAC AGGCTATATT CTGCATCGTG GGACTCTACA GTGAAGGTGT
2281GGTCTGGTGT TCCTGCAGAG ATGCCAGGCA CCAAAAGACA CCACTTTGAC TTGCTGGCCG
2341AGCTGGAACA TGATGTCAGT GTAGATACAA TCAGTTTAAA TGCTGCAAGC ACACTGTTAG
2401TTTCCGGCAC CAAAGAAGGC ACAGTGAATA TTTGGGACCT CACAACGGCC ACCTTAATGC
2461ACCAGATTCC ATGCCATTCA GGGATTGTAT GTGACACTGC TTTTAGCCCA GATAGTCGCC
2521ATGTCCTCAG CACAGGAACA GATGGCTGTC TTAATGTCAT TGATGTGCAG ACAGGAATGC
2581TCATCTCCTC CATGACATCA GATGAGCCCC AGAGGTGCTT TGTCTGGGAT GGAAATTCCG
2641TTTTATCTGG CAGTCAGTCT GGTGAACTGC TCGTTTGGGA CCTCCTTGGA GCAAAAATCA
2701GTGAGAGAAT ACAGGGCCAC ACAGGTGCTG TGACATGTAT ATGGATGAAT GAACAGTGTA
2761GCAGTATCAT CACAGGAGGG GAAGACAGAC AAATTATATT CTGGAAATTG CAGTATTACC
2821CAACTTTCTT GTACAAAGTT GGcattataa gaaagcattg cttatcaatt tgttgcaacg
2881aac

Download FASTA (ORF) (Full)

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