Construct: ORF ccsbBroadEn_07331

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005225.1_s300c1, BRDN0000393342
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TOP3B (8940)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8940 TOP3B DNA topoisomerase III beta NM_001282112.2 99.9% 99.8% 960C>T;1093G>A
2 human 8940 TOP3B DNA topoisomerase III beta NM_001282113.2 99.9% 99.8% 960C>T;1093G>A
3 human 8940 TOP3B DNA topoisomerase III beta NM_001349845.2 99.9% 99.8% 960C>T;1093G>A
4 human 8940 TOP3B DNA topoisomerase III beta NM_001349847.2 99.9% 99.8% 960C>T;1093G>A
5 human 8940 TOP3B DNA topoisomerase III beta NM_003935.5 99.9% 99.8% 960C>T;1093G>A
6 human 8940 TOP3B DNA topoisomerase III beta NM_001349848.2 84.1% 84.1% 0_1ins408;552C>T;685G>A
7 human 8940 TOP3B DNA topoisomerase III beta NM_001349850.2 84.1% 84.1% 0_1ins408;552C>T;685G>A
8 human 8940 TOP3B DNA topoisomerase III beta NM_001349851.2 66.9% 65.9% (many diffs)
9 human 8940 TOP3B DNA topoisomerase III beta NM_001349852.2 66.9% 65.9% (many diffs)
10 human 8940 TOP3B DNA topoisomerase III beta NR_146277.2 66.1% (many diffs)
11 mouse 21976 Top3b topoisomerase (DNA) III beta NM_001326576.1 86.8% 95.4% (many diffs)
12 mouse 21976 Top3b topoisomerase (DNA) III beta NM_011624.3 86.8% 95.4% (many diffs)
13 mouse 21976 Top3b topoisomerase (DNA) III beta XM_006521991.3 86.8% 95.4% (many diffs)
14 mouse 21976 Top3b topoisomerase (DNA) III beta XM_006521992.3 86.8% 95.4% (many diffs)
15 mouse 21976 Top3b topoisomerase (DNA) III beta XM_006521994.3 86.8% 95.4% (many diffs)
16 mouse 21976 Top3b topoisomerase (DNA) III beta XM_006521995.2 86.8% 95.4% (many diffs)
17 mouse 21976 Top3b topoisomerase (DNA) III beta XM_017316937.1 86.8% 95.4% (many diffs)
18 mouse 21976 Top3b topoisomerase (DNA) III beta XM_006521997.3 72.9% 77% (many diffs)
19 mouse 21976 Top3b topoisomerase (DNA) III beta XR_001781810.1 71.6% (many diffs)
20 mouse 21976 Top3b topoisomerase (DNA) III beta XR_384545.3 61.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2652
ORF length:
2586
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGAA GACTGTGCTC ATGGTTGCTG AAAAGCCGTC CTTGGCACAG TCAATTGCCA
121AAATCCTCTC TAGAGGGAGC CTGTCCTCAC ACAAAGGGCT GAACGGGGCC TGCTCAGTCC
181ACGAGTACAC TGGGACCTTT GCTGGCCAGC CAGTGCGCTT CAAGATGACG TCTGTCTGTG
241GTCACGTGAT GACCCTGGAT TTCCTGGGAA AATACAACAA ATGGGACAAA GTGGACCCCG
301CAGAACTGTT CAGCCAAGCT CCCACGGAGA AGAAAGAAGC TAACCCCAAG CTGAACATGG
361TGAAGTTCCT GCAGGTGGAG GGCAGAGGCT GCGACTACAT CGTGCTGTGG CTGGACTGCG
421ACAAGGAGGG GGAGAACATC TGCTTTGAGG TTCTTGATGC TGTTCTGCCC GTCATGAACA
481AGGCCCATGG TGGCGAGAAG ACCGTGTTCC GGGCCAGGTT TAGCTCCATC ACGGACACAG
541ACATCTGTAA TGCCATGGCC TGCCTAGGCG AGCCTGACCA CAACGAGGCG CTCTCAGTGG
601ATGCTCGCCA GGAGCTGGAC CTGCGAATCG GCTGTGCATT CACCAGGTTT CAGACTAAAT
661ATTTCCAGGG GAAATACGGT GATTTAGACA GCTCTCTCAT CTCCTTTGGG CCGTGTCAGA
721CTCCAACCCT GGGATTCTGT GTGGAGAGAC ATGATAAAAT CCAGTCCTTC AAACCAGAGA
781CCTACTGGGT GCTGCAGGCC AAGGTTAACA CTGACAAAGA CAGATCTCTC CTTTTGGACT
841GGGACCGAGT AAGAGTGTTT GACCGGGAGA TCGCACAGAT GTTTTTAAAC ATGACAAAGC
901TGGAGAAGGA AGCCCAGGTG GAGGCCACAA GCAGGAAAGA AAAGGCCAAG CAGAGGCCCC
961TGGCCCTGAA CACTGTGGAG ATGCTGCGTG TGGCCAGCTC TTCTCTGGGC ATGGGGCCGC
1021AGCATGCCAT GCAGACGGCT GAGCGGCTCT ACACGCAAGG CTACATCAGC TACCCACGGA
1081CAGAGACCAC CCACTACCCT GAGAACTTTG ACCTGAAGGG CTCTCTGCGG CAGCAGGCCA
1141ACCACCCCTA CTGGGCCAAC ACGGTGAAGC GGTTGTTAGC AGAAGGTATC AACCGCCCGC
1201GGAAAGGCCA TGACGCCGGC GACCATCCCC CCATCACCCC CATGAAGTCT GCCACAGAGG
1261CCGAATTAGG GGGTGACGCG TGGCGGCTCT ATGAGTACAT CACCAGACAC TTCATCGCCA
1321CGGTCAGCCA TGACTGCAAG TACCTGCAGA GCACCATCTC CTTCAGAATT GGGCCCGAGC
1381TCTTCACCTG CTCCGGGAAG ACCGTCCTCT CACCAGGCTT CACGGAGGTC ATGCCCTGGC
1441AGAGCGTGCC CCTGGAGGAG AGCCTGCCCA CTTGCCAGCG GGGTGATGCC TTCCCTGTGG
1501GCGAGGTGAA GATGCTGGAG AAGCAGACGA ACCCACCCGA CTACCTGACG GAGGCCGAGC
1561TCATCACGCT CATGGAGAAG CATGGCATCG GCACGGATGC CAGCATCCCT GTGCATATCA
1621ACAACATCTG CCAGCGCAAC TATGTCACGG TGGAGAGCGG GCGCCGGCTC AAGCCCACCA
1681ACCTCGGCAT CGTCCTGGTG CACGGCTACT ATAAGATTGA TGCAGAGCTG GTGCTCCCCA
1741CCATCCGCAG TGCAGTGGAG AAGCAGCTGA ACCTGATCGC CCAGGGCAAG GCCGACTACC
1801GCCAGGTCCT GGGCCACACC CTGGACGTGT TCAAGAGGAA GTTCCACTAC TTTGTCGACT
1861CCATTGCTGG CATGGATGAG TTGATGGAGG TGTCTTTCTC GCCCCTGGCG GCCACAGGCA
1921AGCCCCTCTC ACGCTGTGGG AAGTGCCACC GCTTCATGAA GTACATCCAG GCCAAGCCAA
1981GCCGCCTGCA CTGCTCCCAC TGCGATGAGA CCTACACGCT CCCCCAGAAC GGCACCATCA
2041AGCTCTACAA GGAGCTCCGC TGCCCTCTGG ATGACTTCGA GCTGGTCCTG TGGTCATCAG
2101GCTCTCGGGG CAAGAGCTAC CCGCTGTGCC CCTACTGCTA CAACCACCCA CCCTTCCGAG
2161ACATGAAGAA AGGCATGGGC TGCAACGAGT GTACGCACCC CTCCTGCCAG CACTCGCTGA
2221GCATGCTGGG CATCGGCCAG TGCGTGGAAT GTGAGAGCGG GGTGCTGGTG CTGGACCCCA
2281CCTCGGGCCC CAAGTGGAAG GTGGCCTGCA ACAAGTGCAA CGTGGTAGCG CACTGCTTCG
2341AGAACGCCCA CCGCGTGCGG GTGTCCGCCG ACACCTGCAG TGTCTGTGAG GCCGCCTTGC
2401TTGATGTGGA CTTCAACAAG GCCAAGTCCC CACTCCCGGG CGATGAGACG CAGCACATGG
2461GCTGCGTCTT TTGTGACCCC GTCTTCCAGG AGCTGGTGGA GCTGAAGCAT GCGGCCTCCT
2521GCCACCCCAT GCACCGCGGT GGACCAGGGA GAAGGCAGGG TCGAGGGCGG GGCCGGGCCA
2581GGAGGCCCCC TGGGAAGCCC AACCCCAGAC GGCCCAAGGA CAAGATGTCA GCCCTGGCCG
2641CCTACTTTGT ATGCCCAACT TTCTTGTACA AAGTtggcat tataagaaag cattgcttat
2701caatttgttg caacgaac

Download FASTA (ORF) (Full)