Construct: ORF ccsbBroadEn_07363
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009830.1_s300c1, BRDN0000456700
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AIFM1 (9131)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9131 | AIFM1 | apoptosis inducing factor m... | NM_145812.2 | 99.8% | 100% | 261T>C;1803C>A |
| 2 | human | 9131 | AIFM1 | apoptosis inducing factor m... | NM_004208.4 | 95.1% | 95.1% | (many diffs) |
| 3 | human | 9131 | AIFM1 | apoptosis inducing factor m... | NR_132647.1 | 68.4% | (many diffs) | |
| 4 | human | 9131 | AIFM1 | apoptosis inducing factor m... | NM_001130847.3 | 48.6% | 47.7% | (many diffs) |
| 5 | human | 9131 | AIFM1 | apoptosis inducing factor m... | NM_001130846.3 | 44.9% | 44.9% | 0_1ins1005;798C>A |
| 6 | mouse | 26926 | Aifm1 | apoptosis-inducing factor, ... | NM_001290364.1 | 90.4% | 92.1% | (many diffs) |
| 7 | mouse | 26926 | Aifm1 | apoptosis-inducing factor, ... | NM_012019.3 | 86.7% | 87.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1896
- ORF length:
- 1827
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GTTCCGGTGT GGAGGCCTGG CGGCGGGTGC TTTGAAGCAG AAGCTGGTGC 121 CCTTGGTGCG GACCGTGTGC GTCCGAAGCC CGAGGCAGAG GAACCGGCTC CCAGTTGTGC 181 AGTCTCATCA CCTAGGATCC CCTTCTAGAT CACTAGCATC TACAGGTGCT TCTGGGAAAG 241 ATGGCAGCAA CCTAGTGTAC TTCTTAATTG TAGGAGCAAC AGTCACTGGG GCAGGAGTTT 301 ATTATGCCTA CAAGACTATG AAAGAGGACG AAAAAAGATA CAATGAAAGA ATTTCAGGGT 361 TAGGGCTGAC ACCAGAACAG AAACAGAAAA AGGCCGCGTT ATCTGCTTCA GAAGGAGAGG 421 AAGTTCCTCA AGACAAGGCG CCAAGTCATG TTCCTTTCCT GCTAATTGGT GGAGGCACAG 481 CTGCTTTTGC TGCAGCCAGA TCCATCCGGG CTCGGGATCC TGGGGCCAGG GTACTGATTG 541 TATCTGAAGA TCCTGAGCTG CCGTACATGC GACCTCCTCT TTCAAAAGAA CTGTGGTTTT 601 CAGATGACCC AAATGTCACA AAGACACTGC GATTCAAACA GTGGAATGGA AAAGAGAGAA 661 GCATATATTT CCAGCCACCT TCTTTCTATG TCTCTGCTCA GGACCTGCCT CATATTGAGA 721 ATGGTGGTGT GGCTGTCCTC ACTGGGAAGA AGGTAGTACA GCTGGATGTG AGAGACAACA 781 TGGTGAAACT TAATGATGGC TCTCAAATAA CCTATGAAAA GTGCTTGATT GCAACAGGAG 841 GTACTCCAAG AAGTCTGTCT GCCATTGATA GGGCTGGAGC AGAGGTGAAG AGTAGAACAA 901 CGCTTTTCAG AAAGATTGGA GACTTTAGAA GCTTGGAGAA GATTTCACGG GAAGTCAAAT 961 CAATTACGAT TATCGGTGGG GGCTTCCTTG GTAGCGAACT GGCCTGTGCT CTTGGCAGAA 1021 AGGCTCGAGC CTTGGGCACA GAAGTGATTC AACTCTTCCC CGAGAAAGGA AATATGGGAA 1081 AGATCCTCCC CGAATACCTC AGCAACTGGA CCATGGAAAA AGTCAGACGA GAGGGGGTTA 1141 AGGTGATGCC CAATGCTATT GTGCAATCCG TTGGAGTCAG CAGTGGCAAG TTACTTATCA 1201 AGCTGAAAGA CGGCAGGAAG GTAGAAACTG ACCACATAGT GGCAGCTGTG GGCCTGGAGC 1261 CCAATGTTGA GTTGGCCAAG ACTGGTGGCC TGGAAATAGA CTCAGATTTT GGTGGCTTCC 1321 GGGTAAATGC AGAGCTACAA GCACGCTCTA ACATCTGGGT GGCAGGAGAT GCTGCATGCT 1381 TCTACGATAT AAAGTTGGGA AGGAGGCGGG TAGAGCACCA TGATCACGCT GTTGTGAGTG 1441 GAAGATTGGC TGGAGAAAAT ATGACTGGAG CTGCTAAGCC GTACTGGCAT CAGTCAATGT 1501 TCTGGAGTGA TTTGGGCCCC GATGTTGGCT ATGAAGCTAT TGGTCTTGTG GACAGTAGTT 1561 TGCCCACAGT TGGTGTTTTT GCAAAAGCAA CTGCACAAGA CAACCCCAAA TCTGCCACAG 1621 AGCAGTCAGG AACTGGTATC CGATCAGAGA GTGAGACAGA GTCCGAGGCC TCAGAAATTA 1681 CTATTCCTCC CAGCACCCCG GCAGTTCCAC AGGCTCCCGT CCAGGGGGAG GACTACGGCA 1741 AAGGTGTCAT CTTCTACCTC AGGGACAAAG TGGTCGTGGG GATTGTGCTA TGGAACATCT 1801 TTAACCGAAT GCCAATAGCA AGGAAGATCA TTAAGGACGG TGAGCAGCAT GAAGATCTCA 1861 ATGAAGTAGC AAAACTATTC AACATTCATG AAGACTTGCC AACTTTCTTG TACAAAGTTG 1921 Gcattataag aaagcattgc ttatcaattt gttgcaacga ac