Construct: ORF ccsbBroadEn_07468

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016346.1_s300c1, BRDN0000390273
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EMC2 (9694)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9694 EMC2 ER membrane protein complex... NM_014673.5 99.8% 99.6% 434A>G
2 human 9694 EMC2 ER membrane protein complex... NM_001329493.1 96.9% 96.7% 155_181del;461A>G
3 human 9694 EMC2 ER membrane protein complex... NM_001329495.1 96.8% 95.6% (many diffs)
4 human 9694 EMC2 ER membrane protein complex... NM_001329494.1 66% 59% (many diffs)
5 human 9694 EMC2 ER membrane protein complex... NR_138033.1 23.3% (many diffs)
6 mouse 66736 Emc2 ER membrane protein complex... NM_025736.2 91.9% 96.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
957
ORF length:
891
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC GAAGGTCTCA GAGCTTTACG ATGTCACTTG GGAAGAAATG AGAGATAAAA
121TGAGAAAATG GAGAGAAGAA AACTCAAGAA ATAGTGAGCA AATTGTGGAA GTTGGAGAAG
181AATTAATTAA TGAATATGCT TCTAAGCTGG GAGATGATAT TTGGATCATA TATGAACAGG
241TGATGATTGC AGCACTAGAC TATGGTCGGG ATGACTTGGC ATTGTTTTGT CTTCAAGAGC
301TGAGAAGACA GTTCCCTGGC AGTCACAGAG TCAAGCGATT AACAGGCATG AGATTTGAAG
361CCATGGAAAG ATATGATGAT GCTATACAGC TATATGATAG GATTTTACAA GAAGATCCAA
421CTAACACTGC TGCAAGAAAG CGTAAGATTG CCATTCGAAA AGCCCAGGGG AAAAATGTGG
481AGGCCATTCG GGAGCTGAGT GAGTATCTGG AACAATTTGT TGGAGACCAA GAAGCCTGGC
541ATGAACTTGC AGAACTTTAC ATCAATGAAC ATGACTATGC AAAAGCAGCC TTTTGTTTAG
601AGGAACTAAT GATGACTAAT CCACACAACC ACTTATACTG TCAGCAGTAT GCTGAAGTTA
661AGTATACCCA AGGTGGACTT GAAAACCTCG AACTTTCAAG AAAGTATTTT GCACAGGCAT
721TGAAACTGAA CAACAGAAAT ATGAGAGCTT TGTTTGGACT TTATATGTCG GCAAGTCATA
781TTGCTTCTAA TCCAAAAGCA AGTGCAAAAA CGAAAAAGGA CAACATGAAA TATGCTAGTT
841GGGCAGCTAG TCAAATAAAC AGAGCTTATC AGTTTGCAGG TCGAAGTAAG AAGGAAACCA
901AATATTCTCT TAAGGCTGTC GAAGACATGT TGGAAACATT GCAGATCACC CAGTCTTACC
961CAACTTTCTT GTACAAAGTt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
1021aac

Download FASTA (ORF) (Full)