Construct: ORF ccsbBroadEn_07583
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013400.1_s300c1, BRDN0000457274
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MARCHF6 (10299)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10299 | MARCHF6 | membrane associated ring-CH... | NM_005885.4 | 99.9% | 100% | 2709A>G |
| 2 | human | 10299 | MARCHF6 | membrane associated ring-CH... | NM_001270660.2 | 94.6% | 94.6% | 190_191ins144;2565A>G |
| 3 | human | 10299 | MARCHF6 | membrane associated ring-CH... | XM_011513932.2 | 92.4% | 92.5% | 0_1ins204;2505A>G |
| 4 | human | 10299 | MARCHF6 | membrane associated ring-CH... | NM_001270661.2 | 87.8% | 87.6% | (many diffs) |
| 5 | human | 10299 | MARCHF6 | membrane associated ring-CH... | XM_011513934.2 | 85.3% | 84.3% | (many diffs) |
| 6 | human | 10299 | MARCHF6 | membrane associated ring-CH... | XR_925577.2 | 82.2% | (many diffs) | |
| 7 | human | 10299 | MARCHF6 | membrane associated ring-CH... | XM_011513936.3 | 69.4% | 69.3% | 1898C>T;1900_1908delAAAAAGGCCinsT;1911_1912ins827 |
| 8 | human | 10299 | MARCHF6 | membrane associated ring-CH... | XR_925576.3 | 55.7% | (many diffs) | |
| 9 | human | 10299 | MARCHF6 | membrane associated ring-CH... | XM_017008944.2 | 49.8% | 48.9% | (many diffs) |
| 10 | mouse | 223455 | March6 | membrane-associated ring fi... | NM_172606.2 | 90.4% | 97.9% | (many diffs) |
| 11 | mouse | 223455 | March6 | membrane-associated ring fi... | XM_011245351.2 | 89.9% | 97.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2799
- ORF length:
- 2730
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGACACCGCG GAGGAAGACA TATGTAGAGT GTGTCGGTCA GAAGGAACAC 121 CTGAGAAACC GCTTTATCAT CCTTGTGTAT GTACTGGCAG TATTAAGTTT ATCCATCAAG 181 AATGCTTAGT TCAATGGCTG AAACACAGTC GAAAAGAATA CTGTGAATTA TGCAAGCACA 241 GATTTGCTTT TACACCAATT TATTCTCCAG ATATGCCTTC ACGGCTTCCA ATTCAAGACA 301 TATTTGCTGG ACTGGTTACA AGTATTGGCA CTGCAATACG ATATTGGTTT CATTATACAC 361 TTGTGGCCTT TGCATGGTTG GGAGTTGTTC CTCTTACAGC ATGCCGCATC TACAAGTGCT 421 TGTTTACTGG CTCCGTGAGC TCACTACTGA CGCTGCCATT AGATATGCTG TCAACGGAAA 481 ATTTGTTGGC AGATTGTTTG CAGGGTTGTT TTGTGGTGAC GTGCACACTG TGTGCATTCA 541 TCAGCCTGGT GTGGTTGAGA GAGCAGATAG TCCATGGGGG AGCACCAATT TGGTTGGAGC 601 ATGCTGCCCC ACCGTTCAAT GCTGCGGGGC ATCACCAAAA TGAGGCTCCA GCAGGAGGAA 661 ATGGTGCAGA AAATGTTGCT GCTGATCAGC CTGCTAACCC ACCAGCTGAG AACGCAGTGG 721 TGGGGGAAAA CCCTGATGCC CAGGATGACC AGGCAGAAGA GGAGGAGGAG GACAATGAGG 781 AGGAAGATGA CGCTGGTGTG GAGGATGCGG CAGATGCTAA TAACGGAGCC CAGGATGACA 841 TGAATTGGAA TGCTTTAGAA TGGGACCGAG CTGCTGAAGA GCTTACATGG GAAAGAATGC 901 TAGGACTTGA TGGATCACTA GTTTTTCTGG AACATGTCTT CTGGGTGGTA TCTTTAAATA 961 CACTGTTCAT TCTTGTTTTT GCATTTTGCC CTTACCATAT TGGTCATTTC TCCCTTGTTG 1021 GTTTGGGATT TGAAGAACAC GTCCAAGCAT CTCATTTTGA AGGCCTAATC ACAACCATAG 1081 TTGGGTATAT ACTTTTAGCA ATAACACTGA TAATTTGTCA TGGCTTGGCA ACTCTTGTGA 1141 AATTTCATAG ATCTCGTCGC TTACTGGGAG TCTGCTATAT TGTTGTTAAG GTCTCTTTGT 1201 TAGTGGTGGT AGAAATTGGA GTATTCCCTC TCATTTGTGG TTGGTGGCTG GATATCTGTT 1261 CCTTGGAAAT GTTTGATGCT ACTCTGAAAG ATCGAGAACT GAGCTTTCAG TCGGCTCCAG 1321 GTACTACCAT GTTTCTGCAT TGGCTAGTGG GAATGGTATA TGTCTTCTAC TTTGCCTCCT 1381 TCATTCTACT ACTGAGAGAG GTACTTCGAC CTGGTGTCCT GTGGTTTCTA AGGAATTTGA 1441 ATGATCCAGA TTTCAATCCA GTACAGGAAA TGATCCATTT GCCAATATAT AGGCATCTCC 1501 GAAGATTTAT TTTGTCAGTG ATTGTCTTTG GCTCCATTGT CCTCCTGATG CTTTGGCTTC 1561 CTATACGTAT AATTAAGAGT GTGCTGCCTA ATTTTCTTCC ATACAATGTC ATGCTCTACA 1621 GTGATGCTCC AGTGAGTGAA CTGTCCCTCG AGCTGCTTCT GCTTCAGGTT GTCTTGCCAG 1681 CATTACTCGA ACAGGGACAC ACGAGGCAGT GGCTGAAGGG GCTGGTGCGA GCGTGGACTG 1741 TGACCGCCGG ATACTTGCTG GATCTTCATT CTTATTTATT GGGAGACCAG GAAGAAAATG 1801 AAAACAGTGC AAATCAACAA GTTAACAATA ATCAGCATGC TCGAAATAAC AACGCTATTC 1861 CTGTGGTGGG AGAAGGCCTT CATGCAGCCC ACCAAGCCAT ACTCCAGCAG GGAGGGCCTG 1921 TTGGCTTTCA GCCTTACCGC CGACCTTTAA ATTTTCCACT CAGGATATTT CTGTTGATTG 1981 TCTTCATGTG TATAACATTA CTGATTGCCA GCCTCATCTG CCTTACTTTA CCAGTATTTG 2041 CTGGCCGTTG GTTAATGTCG TTTTGGACGG GGACTGCCAA AATCCATGAG CTCTACACAG 2101 CTGCTTGTGG TCTCTATGTT TGCTGGCTAA CCATAAGGGC TGTGACGGTG ATGGTGGCAT 2161 GGATGCCTCA GGGACGCAGA GTGATCTTCC AGAAGGTTAA AGAGTGGTCT CTCATGATCA 2221 TGAAGACTTT GATAGTTGCG GTGCTGTTGG CTGGAGTTGT CCCTCTCCTT CTGGGGCTCC 2281 TGTTTGAGCT GGTCATTGTG GCTCCCCTGA GGGTTCCCTT GGATCAGACT CCTCTTTTTT 2341 ATCCATGGCA GGACTGGGCA CTTGGAGTCC TGCATGCCAA AATCATTGCA GCTATAACAT 2401 TGATGGGTCC TCAGTGGTGG TTGAAAACTG TAATTGAACA GGTTTACGCA AATGGCATCC 2461 GGAACATTGA CCTTCACTAT ATTGTTCGTA AACTGGCAGC TCCCGTGATC TCTGTGCTGT 2521 TGCTTTCCCT GTGTGTACCT TATGTCATAG CTTCTGGTGT TGTTCCTTTA CTAGGTGTTA 2581 CTGCGGAAAT GCAAAACTTA GTCCATCGGC GGATTTATCC ATTTTTACTG ATGGTCGTGG 2641 TATTGATGGC AATTTTGTCC TTCCAAGTCC GCCAGTTTAA GCGCCTTTAT GAACATATTA 2701 AAAATGACAA GTACCTTGTG GGTCAACGAC TCGTGAACTA CGAACGGAAA TCTGGCAAAC 2761 AAGGCTCATC TCCACCGCCT CCACAGTCAT CCCAAGAATT GCCAACTTTC TTGTACAAAG 2821 TTGGcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac