Construct: ORF ccsbBroadEn_07781

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015949.1_s300c1, BRDN0000387540
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HPS5 (11234)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11234 HPS5 HPS5 biogenesis of lysosoma... NM_007216.4 99.9% 99.9% 480C>A;2704G>A
2 human 11234 HPS5 HPS5 biogenesis of lysosoma... NM_181508.1 99.9% 99.9% 480C>A;2704G>A
3 human 11234 HPS5 HPS5 biogenesis of lysosoma... XM_017017154.1 99.9% 99.9% 480C>A;2704G>A
4 human 11234 HPS5 HPS5 biogenesis of lysosoma... XM_011519866.1 96.8% 95% (many diffs)
5 human 11234 HPS5 HPS5 biogenesis of lysosoma... XM_011519867.1 96.8% 95% (many diffs)
6 human 11234 HPS5 HPS5 biogenesis of lysosoma... XM_011519868.3 96.8% 95% (many diffs)
7 human 11234 HPS5 HPS5 biogenesis of lysosoma... XM_017017152.1 92.8% 92.8% 1_231del;711C>A;2935G>A
8 human 11234 HPS5 HPS5 biogenesis of lysosoma... XM_017017153.2 92.8% 92.8% 1_231del;711C>A;2935G>A
9 human 11234 HPS5 HPS5 biogenesis of lysosoma... XM_011519865.1 90.1% 88.5% (many diffs)
10 human 11234 HPS5 HPS5 biogenesis of lysosoma... XM_017017151.2 90.1% 88.5% (many diffs)
11 human 11234 HPS5 HPS5 biogenesis of lysosoma... NM_181507.2 89.8% 89.8% 1_342del;822C>A;3046G>A
12 human 11234 HPS5 HPS5 biogenesis of lysosoma... XM_017017149.1 89.8% 89.8% 1_342del;822C>A;3046G>A
13 human 11234 HPS5 HPS5 biogenesis of lysosoma... XM_017017150.1 89.8% 89.8% 1_342del;822C>A;3046G>A
14 human 11234 HPS5 HPS5 biogenesis of lysosoma... XM_011519862.1 87.2% 85.7% (many diffs)
15 human 11234 HPS5 HPS5 biogenesis of lysosoma... XM_011519863.1 87.2% 85.7% (many diffs)
16 human 11234 HPS5 HPS5 biogenesis of lysosoma... XM_011519864.1 87.2% 85.7% (many diffs)
17 human 11234 HPS5 HPS5 biogenesis of lysosoma... XR_001747753.1 61.9% (many diffs)
18 human 11234 HPS5 HPS5 biogenesis of lysosoma... XR_001747751.1 61.7% (many diffs)
19 human 11234 HPS5 HPS5 biogenesis of lysosoma... XR_001747752.1 61.7% (many diffs)
20 human 11234 HPS5 HPS5 biogenesis of lysosoma... XR_001747750.1 60.3% (many diffs)
21 human 11234 HPS5 HPS5 biogenesis of lysosoma... XR_001747754.2 50.9% (many diffs)
22 human 11234 HPS5 HPS5 biogenesis of lysosoma... XR_001747755.2 49.3% (many diffs)
23 human 11234 HPS5 HPS5 biogenesis of lysosoma... XR_001747756.2 48.5% (many diffs)
24 mouse 246694 Hps5 HPS5, biogenesis of lysosom... XM_006540907.3 36.1% 35.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3111
ORF length:
3045
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTA TGTGTCTTCA GAACACAAAG GCCGAAGAGT CACAGCTCTC TGCTGGGATA
121CAGCTATTCT TAGAGTTTTT GTAGGTGATC ATGCTGGGAA GGTTTCTGCT ATCAAACTCA
181ATACTTCTAA ACAAGCAAAG GCAGCTGCTG CTTTTGTGAT GTTTCCTGTT CAGACAATCA
241CAACTGTTGA CTCCTGTGTT GTACAGTTAG ATTATTTGGA TGGAAGGCTA CTTATATCTT
301CACTTACTCG ATCCTTCTTG TGTGACACTG AGAGAGAAAA GTTTTGGAAA ATTGGAAACA
361AGGAAAGAGA TGGAGAATAT GGAGCTTGTT TCTTTCCTGG AAGATGTTCT GGGGGCCAGC
421AACCTCTGAT ATATTGTGCT CGCCCAGGCT CTAGGATGTG GGAAGTGAAC TTTGATGGAG
481AAGTTATAAG TACACATCAG TTCAAGAAAC TCCTCTCGTT GCCACCTCTC CCTGTGATTA
541CTCTAAGATC AGAACCTCAG TATGATCATA CAGCTGGATC CTCCCAGTCT TTGTCTTTCC
601CCAAACTCTT ACATCTTAGT GAGCATTGTG TGCTGACTTG GACAGAAAGA GGAATTTATA
661TTTTCATTCC TCAGAATGTT CAAGTTCTTC TTTGGAGTGA AGTCAAAGAT ATTCAGGATG
721TGGCTGTCTG TAGGAATGAA TTGTTCTGTT TGCACCTAAA TGGGAAAGTC TCACATCTCT
781CCCTGATATC TGTGGAGCGC TGTGTGGAAC GCCTGCTAAG AAGAGGCCTA TGGAACTTGG
841CTGCTCGTAC ATGCTGTCTT TTCCAAAATT CTGTCATTGC CAGCAGAGCA AGAAAAACTT
901TGACTGCAGA TAAATTGGAG CATTTGAAAT CTCAGCTGGA CCATGGCACC TACAATGATC
961TAATTTCTCA ACTGGAAGAA TTGATCTTAA AATTTGAACC TTTGGATTCA GCTTGTAGCA
1021GTAGAAGAAG CTCCATTTCA TCACATGAAA GTTTCAGCAT CTTGGACTCT GGTATTTATC
1081GTATCATTAG TAGTAGAAGA GGCAGTCAGT CAGATGAAGA CTCTTGCTCC CTTCACAGCC
1141AAACCCTCTC AGAAGATGAG AGATTTAAAG AATTCACCTC ACAGCAGGAA GAGGACCTGC
1201CAGATCAGTG TTGTGGCTCA CACGGAAATG AAGACAATGT TTCTCATGCT CCAGTGATGT
1261TTGAGACAGA TAAGAATGAA ACTTTTCTCC CGTTCGGCAT TCCATTACCA TTTCGTTCTC
1321CATCTCCTCT TGTGTCTCTT CAGGCTGTCA AAGAAAGTGT TTCTAGCTTT GTGCGTAAAA
1381CTACTGAGAA GATTGGCACC CTTCACACGA GCCCTGATCT GAAAGTGAGA CCAGAGCTCA
1441GGGGTGATGA GCAATCATGT GAAGAGGATG TGAGTTCAGA TACCTGCCCA AAGGAGGAAG
1501ACACTGAGGA GGAAAAAGAG GTAACTAGTC CACCTCCAGA AGAAGACAGG TTCCAGGAGC
1561TTAAAGTAGC AACAGCAGAA GCAATGACCA AGCTACAGGA CCCTCTGGTT TTATTTGAAT
1621CCGAGTCTCT GAGAATGGTT TTACAGGAGT GGCTTTCACA TTTAGAAAAA ACATTTGCCA
1681TGAAGGACTT TTCAGGTGTT TCAGATACTG ACAACTCATC CATGAAATTG AACCAGGATG
1741TGCTATTAGT TAATGAATCA AAAAAGGGAA TATTAGATGA AGATAATGAA AAAGAAAAAA
1801GGGACTCTTT AGGCAATGAA GAATCTGTTG ATAAAACAGC ATGTGAATGT GTAAGGAGTC
1861CAAGGGAGTC TTTGGATGAC CTGTTTCAAA TATGTTCTCC ATGCGCCATT GCAAGTGGTC
1921TTCGGAACGA CCTGGCTGAA TTGACAACAT TATGTTTGGA GTTGAATGTA TTGAATTCTA
1981AGATCAAAAG CACCAGTGGA CATGTGGACC ACACTTTGCA ACAGTACTCT CCTGAAATTC
2041TGGCTTGCCA GTTCCTGAAG AAGTACTTTT TTCTCCTGAA CTTGAAAAGA GCGAAGGAGA
2101GTATCAAGCT TAGTTACAGT AATAGCCCTT CTGTTTGGGA TACTTTTATT GAAGGATTGA
2161AAGAAATGGC AAGTTCCAAT CCTGTGTATA TGGAGATGGA AAAAGGAGAT CTACCAACAA
2221GGTTAAAGTT ACTAGATGAC GAGGTTCCTT TTGATAGTCC GTTGTTGGTT GTTTATGCTA
2281CCCGGTTGTA TGAAAAGTTT GGGGAGTCTG CTCTTCGATC CTTAATCAAG TTCTTTCCAT
2341CCATTTTGCC ATCGGATATC ATACAACTTT GTCATCATCA TCCTGCTGAG TTTTTGGCCT
2401ATTTAGACAG TCTGGTGAAA TCAAGGCCTG AAGATCAGCG GTCATCTTTT CTTGAGTCCC
2461TTCTGCAACC AGAGTCTTTA AGGTTGGATT GGCTGCTTTT GGCAGTGTCC CTTGATGCTC
2521CACCAAGCAC CAGCACAATG GATGATGAAG GTTATCCCAG GCCTCATTCA CACTTGCTTT
2581CCTGGGGTTA CAGTCAGCTG ATCCTTCATC TAATTAAACT TCCTGCAGAT TTTATAACCA
2641AAGAGAAAAT GACAGACATC TGCAGGTCTT GTGGTTTCTG GCCTGGATAT CTAATTCTCT
2701GTTTGGAGCT GGAGAGAAGA AGAGAGGCCT TCACCAATAT TGTGTATCTG AATGATATGA
2761GCCTGATGAA AGGGGACAAT GGTTGGATCC CAGAGACCGT GGAGGAATGG AAGCTTCTCC
2821TTCATCTCAT ACAGAGCAAG AGCACGAGGC CAGCCCCCCA GGAGTCACTA AATGGGAGCC
2881TCAGTGATGG GCCTTCCCCC ATCAATGTGG AGAATGTGGC ACTTCTGTTA GCTAAGGCCA
2941TGGGCCCAGA TCGGGCTTGG TCACTGCTAC AGGAATGTGG TCTGGCCCTT GAGTTGTCAG
3001AGAAGTTTAC CAGAACCTGC GATATCCTGA GGATTGCTGA GAAAAGGCAG AGGGCCTTGA
3061TACAAAGCAT GCTTGAAAAA TGCGATCGGT TTCTCTGGTC CCAGCAGGCC TACCCAACTT
3121TCTTGTACAA AGTtggcatt ataagaaagc attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)