Construct: ORF ccsbBroadEn_08014

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015342.1_s300c1, BRDN0000456187
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GBGT1 (26301)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26301 GBGT1 globoside alpha-1,3-N-acety... NM_021996.6 99.8% 99.7% 58C>T;987C>T
2 human 26301 GBGT1 globoside alpha-1,3-N-acety... NM_001282632.2 94.9% 94.5% 58C>T;137_138ins51;936C>T
3 human 26301 GBGT1 globoside alpha-1,3-N-acety... NM_001288572.2 86.3% 86.4% 0_1ins141;846C>T
4 human 26301 GBGT1 globoside alpha-1,3-N-acety... NM_001282629.2 84.6% 38.2% 58C>T;359_360insGTACACTCATTTCATCCAG;882_883ins140
5 human 26301 GBGT1 globoside alpha-1,3-N-acety... NM_001288573.2 60.7% 60.8% 0_1ins408;579C>T
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1107
ORF length:
1041
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCA TCGCCGGAGA CTGGCCCTGG GTCTGGGGTT CTGCCTGTTG GCGGGCACAA
121GCTTCAGTGT CCTGTGGGTG TATCTTGAGA ACTGGCTGCC AGTCTCCTAT GTCCCCTATT
181ATCTCCCCTG CCCAGAGATC TTCAACATGA AGCTGCACTA CAAGAGGGAG AAGCCACTCC
241AGCCCGTGGT ATGGTCACAG TACCCTCAGC CCAAGCTGCT GGAGCACAGG CCCACACAGC
301TGCTGACACT CACACCCTGG TTGGCGCCCA TCGTCTCCGA GGGAACCTTC AACCCAGAGC
361TTCTGCAGCA CATCTACCAG CCACTGAACC TGACCATTGG GGTCACGGTG TTTGCCGTGG
421GGAAGTACAC TCATTTCATC CAGTCCTTCC TGGAGTCAGC CGAGGAGTTC TTCATGCGTG
481GGTACCGGGT GCACTACTAC ATCTTCACTG ACAACCCTGC AGCCGTTCCC GGGGTCCCGC
541TGGGTCCCCA CCGGCTTCTC AGCTCCATCC CCATCCAGGG TCACTCCCAC TGGGAGGAGA
601CATCCATGCG CCGGATGGAG ACCATCAGCC AGCACATTGC TAAGAGGGCT CACCGGGAGG
661TGGACTACCT CTTCTGCCTT GATGTGGACA TGGTGTTTCG GAACCCGTGG GGCCCTGAGA
721CCTTGGGAGA CCTGGTGGCT GCCATTCACC CAAGCTACTA CGCCGTTCCC CGCCAGCAGT
781TCCCCTATGA GCGCAGGCGT GTTTCCACTG CCTTTGTGGC AGACAGCGAA GGGGACTTCT
841ATTATGGTGG GGCAGTCTTC GGGGGGCAGG TGGCCAGGGT ATATGAGTTT ACTAGGGGCT
901GCCACATGGC CATCCTGGCG GACAAGGCCA ATGGCATCAT GGCTGCCTGG CGGGAGGAAA
961GCCACCTGAA CCGTCACTTC ATCTCAAACA AGCCGTCCAA GGTGCTGTCC CCCGAGTACC
1021TCTGGGACGA CAGGAAGCCC CAGCCACCCA GTCTGAAGCT GATCCGCTTT TCTACACTGG
1081ACAAGGATAT CAGCTGCCTG AGGAGCTGCC CAACTTTCTT GTACAAAGTT GGcattataa
1141gaaagcattg cttatcaatt tgttgcaacg aac

Download FASTA (ORF) (Full)