Transcript: Human NM_001288573.2

Homo sapiens globoside alpha-1,3-N-acetylgalactosaminyltransferase 1 (FORS blood group) (GBGT1), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
GBGT1 (26301)
Length:
1991
CDS:
727..1362

Additional Resources:

NCBI RefSeq record:
NM_001288573.2
NBCI Gene record:
GBGT1 (26301)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001288573.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000372286 ATTGCCCTTAACGTAGCATTT pLKO_005 1718 3UTR 100% 10.800 15.120 N GBGT1 n/a
2 TRCN0000036288 GCCAGGGTATATGAGTTTACT pLKO.1 1126 CDS 100% 5.625 7.875 N GBGT1 n/a
3 TRCN0000372339 GTACCGGGTGCACTACTACAT pLKO_005 735 CDS 100% 0.000 0.000 N GBGT1 n/a
4 TRCN0000372338 CCCAACCACATGTAGCCAATT pLKO_005 1811 3UTR 100% 10.800 7.560 N GBGT1 n/a
5 TRCN0000036284 CCGTCACTTCATCTCAAACAA pLKO.1 1224 CDS 100% 5.625 3.938 N GBGT1 n/a
6 TRCN0000036286 CCTGTGGGTGTATCTTGAGAA pLKO.1 347 5UTR 100% 4.950 3.465 N GBGT1 n/a
7 TRCN0000036287 CTTCTGCCTTGATGTGGACAT pLKO.1 924 CDS 100% 4.050 2.835 N GBGT1 n/a
8 TRCN0000036285 GCCCGTGGTATGGTCACAGTA pLKO.1 458 5UTR 100% 1.650 1.155 N GBGT1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001288573.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08014 pDONR223 100% 60.7% 60.8% None 0_1ins408;579C>T n/a
2 ccsbBroad304_08014 pLX_304 0% 60.7% 60.8% V5 0_1ins408;579C>T n/a
3 TRCN0000479602 CCTATTCGAATTCCTTACTCGTTT pLX_317 33.8% 60.7% 60.8% V5 0_1ins408;579C>T n/a
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