Construct: ORF ccsbBroadEn_08117
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009020.1_s300c1, BRDN0000385333
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BLNK (29760)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29760 | BLNK | B cell linker | NM_013314.3 | 99.9% | 99.7% | 184G>C |
| 2 | human | 29760 | BLNK | B cell linker | XM_011539728.2 | 97.1% | 96.9% | (many diffs) |
| 3 | human | 29760 | BLNK | B cell linker | NM_001114094.1 | 94.8% | 94.7% | 184G>C;605_606ins69 |
| 4 | human | 29760 | BLNK | B cell linker | XM_017016159.1 | 92.1% | 91.8% | (many diffs) |
| 5 | human | 29760 | BLNK | B cell linker | NM_001258440.1 | 88.5% | 88.3% | 184G>C;1093_1094ins156 |
| 6 | human | 29760 | BLNK | B cell linker | NM_001258441.1 | 83.4% | 83.3% | 184G>C;605_606ins69;1024_1025ins156 |
| 7 | human | 29760 | BLNK | B cell linker | NR_047680.1 | 67.3% | (many diffs) | |
| 8 | human | 29760 | BLNK | B cell linker | NM_001258442.1 | 65.4% | 65.3% | 184G>C;347_348ins315;778_779ins156 |
| 9 | human | 29760 | BLNK | B cell linker | NR_047682.1 | 64.1% | (many diffs) | |
| 10 | human | 29760 | BLNK | B cell linker | NR_047681.1 | 63.9% | (many diffs) | |
| 11 | human | 29760 | BLNK | B cell linker | NR_047683.1 | 60.5% | (many diffs) | |
| 12 | mouse | 17060 | Blnk | B cell linker | NM_008528.4 | 83.9% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1434
- ORF length:
- 1368
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGA CAAGCTTAAT AAAATAACCG TCCCCGCCAG TCAGAAGTTG AGGCAGCTTC 121 AAAAGATGGT CCATGATATT AAAAACAATG AAGGTGGAAT AATGAATAAA ATCAAAAAGC 181 TAAAAGTCAA AGCACCTCCA AGTGTTCCTC GAAGGGACTA CGCTTCAGAG AGCCCTGCTG 241 ACGAAGAGCA GCAGTGGTCC GATGACTTTG ACAGCGACTA TGAAAATCCA GATGAGCACT 301 CGGACTCAGA GATGTACGTG ATGCCCGCCG AGGAGAACGC TGATGACAGC TACGAGCCGC 361 CTCCAGTAGA GCAGGAAACC AGGCCGGTTC ACCCAGCCCT GCCCTTCGCC AGAGGCGAGT 421 ATATAGACAA TCGATCAAGC CAGAGGCATT CCCCACCCTT CAGCAAGACA CTTCCCAGTA 481 AGCCCAGCTG GCCTTCAGAG AAAGCAAGGC TCACCTCCAC CCTGCCGGCC CTGACTGCTT 541 TGCAGAAACC TCAAGTCCCA CCCAAACCCA AAGGCCTCCT TGAGGATGAG GCTGATTATG 601 TGGTCCCCGT GGAAGATAAT GATGAAAACT ATATTCATCC CACAGAAAGC AGTTCACCTC 661 CACCTGAAAA AGCTCCCATG GTGAATAGAT CAACCAAGCC AAATTCCTCA ACGCCCGCCT 721 CTCCTCCAGG AACAGCTTCA GGTCGAAACA GTGGGGCCTG GGAAACCAAG TCACCTCCAC 781 CAGCTGCACC ATCCCCGTTG CCACGGGCCG GGAAAAAACC AACGACACCA CTGAAGACAA 841 CTCCAGTTGC CTCTCAACAG AATGCTTCAA GTGTTTGTGA AGAAAAACCT ATACCTGCTG 901 AACGCCACCG AGGGTCAAGT CACAGACAAG AAGCTGTGCA GTCACCAGTG TTTCCTCCTG 961 CCCAGAAACA AATCCACCAA AAACCCATAC CTCTGCCAAG ATTTACAGAA GGGGGAAACC 1021 CAACTGTGGA TGGGCCCCTA CCCAGCTTTT CATCTAATTC CACTATTTCA GAACAGGAAG 1081 CTGGCGTTCT CTGCAAGCCA TGGTATGCTG GAGCCTGTGA TCGAAAGTCT GCTGAAGAGG 1141 CATTGCACAG ATCAAACAAG GATGGATCAT TTCTTATTCG GAAAAGCTCT GGCCATGATT 1201 CCAAACAACC ATATACACTA GTTGTATTCT TTAATAAGCG AGTATATAAT ATTCCTGTGC 1261 GATTTATTGA AGCAACAAAA CAATATGCCT TGGGCAGAAA GAAAAATGGT GAAGAGTACT 1321 TTGGAAGTGT TGCTGAAATC ATCAGGAATC ATCAACATAG TCCTTTGGTT CTTATTGACA 1381 GTCAGAATAA CACAAAAGAT TCCACCAGAC TGAAGTATGC AGTTAAAGTT TCATAAAcaa 1441 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac