Construct: ORF ccsbBroadEn_08126

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003599.1_s300c1, BRDN0000456678
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GRHL1 (29841)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29841 GRHL1 grainyhead like transcripti... NM_198182.3 99.8% 99.6% 1051A>G;1189G>A
2 human 29841 GRHL1 grainyhead like transcripti... XM_006711882.3 97.9% 97.6% 1051A>G;1189G>A;1322_1357del
3 human 29841 GRHL1 grainyhead like transcripti... XM_011510343.2 90.5% 90.1% (many diffs)
4 human 29841 GRHL1 grainyhead like transcripti... XM_017003901.1 75.4% 75.2% 0_1ins453;598A>G;736G>A
5 human 29841 GRHL1 grainyhead like transcripti... XM_017003900.1 74% 73.6% (many diffs)
6 human 29841 GRHL1 grainyhead like transcripti... XM_005246159.4 69.3% 58.4% (many diffs)
7 human 29841 GRHL1 grainyhead like transcripti... XM_006711884.4 67.9% 57.1% (many diffs)
8 human 29841 GRHL1 grainyhead like transcripti... XR_001738727.2 49.5% (many diffs)
9 human 29841 GRHL1 grainyhead like transcripti... XR_001738726.2 49% (many diffs)
10 human 29841 GRHL1 grainyhead like transcripti... XR_001738728.2 47.5% (many diffs)
11 mouse 195733 Grhl1 grainyhead-like 1 (Drosophila) NM_001161406.1 88% 94.6% (many diffs)
12 mouse 195733 Grhl1 grainyhead-like 1 (Drosophila) XM_006515016.3 87.7% 94.3% (many diffs)
13 mouse 195733 Grhl1 grainyhead-like 1 (Drosophila) XM_006515017.3 81.2% 87% (many diffs)
14 mouse 195733 Grhl1 grainyhead-like 1 (Drosophila) NM_145890.2 75.8% 80.2% (many diffs)
15 mouse 195733 Grhl1 grainyhead-like 1 (Drosophila) XM_017315004.1 55.2% 57.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1923
ORF length:
1854
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTAGCACCAT GACACAGGAG TACGACAACA AACGGCCAGT GTTGGTTCTT CAGAATGAAG
121CACTTTATCC ACAGCGGCGG TCCTACACTA GTGAGGATGA GGCCTGGAAA TCCTTCCTGG
181AAAACCCTCT CACTGCAGCG ACCAAAGCGA TGATGAGCAT CAATGGAGAT GAAGACAGCG
241CCGCTGCGCT GGGCCTGCTC TATGACTACT ACAAGGTTCC AAGAGAGAGA AGGTCATCAA
301CAGCAAAGCC AGAGGTGGAG CACCCTGAGC CAGATCACAG CAAAAGAAAC AGCATACCAA
361TTGTGACAGA GCAGCCCCTC ATCTCTGCTG GAGAAAACAG AGTGCAAGTA CTGAAAAATG
421TGCCATTTAA CATTGTCCTT CCCCATGGCA ACCAGCTGGG CATTGATAAG AGAGGCCATC
481TGACAGCTCC AGATACGACA GTCACTGTCT CCATAGCAAC GATGCCTACC CACTCCATCA
541AGACAGAAAC CCAGCCACAT GGCTTCGCTG TGGGAATCCC CCCAGCAGTG TATCATCCTG
601AGCCCACTGA GCGGGTGGTG GTTTTCGATC GGAATCTCAA TACTGACCAG TTCAGCTCTG
661GTGCTCAAGC CCCAAATGCT CAAAGGCGAA CTCCAGACTC GACCTTCTCA GAGACCTTCA
721AGGAAGGCGT TCAGGAGGTT TTCTTCCCCT CGGATCTCAG TCTGCGGATG CCTGGCATGA
781ATTCAGAGGA CTATGTTTTT GACAGTGTTT CTGGGAACAA CTTTGAATAT ACCCTAGAAG
841CTTCAAAATC ACTTCGACAG AAGCCAGGAG ACAGTACCAT GACGTACCTG AACAAAGGCC
901AGTTCTATCC CATCACCTTG AAGGAGGTGA GCAGCAGTGA AGGAATCCAT CATCCCATCA
961GCAAAGTTCG AAGTGTGATC ATGGTGGTTT TTGCTGAAGA CAAAAGCAGA GAAGATCAGT
1021TAAGGCATTG GAAGTACTGG CACTCCCGGC AGCACACCGC TAAACAAAGA TGCATTGACA
1081TAGCTGACTA TAAAGAAAGC TTCAACACTA TCAGTAACGT CGAGGAGATT GCGTATAACG
1141CCATTTCCTT CACATGGGAC ATCAACGATG AAGCAAAGGT TTTCATCTCT GTGAACTGCT
1201TAAGCACAGA TTTCTCTTCC CAGAAGGGAG TGAAGGGGTT GCCTCTTAAC ATTCAAATTG
1261ATACCTATAG TTACAACAAC CGCAGCAACA AGCCTGTGCA CCGGGCCTAC TGCCAGATCA
1321AGGTCTTCTG TGACAAGGGA GCTGAGCGGA AAATCAGGGA TGAAGAACGA AAGCAAAGCA
1381AAAGAAAAGT TTCTGATGTT AAAGTGCCAC TGCTTCCCTC TCACAAGCGA ATGGATATCA
1441CAGTTTTCAA ACCCTTCATT GATCTCGATA CTCAGCCTGT CCTCTTCATT CCTGACGTGC
1501ACTTTGCCAA CTTGCAGCGG GGCACTCATG TCCTTCCCAT TGCCTCTGAA GAATTGGAGG
1561GTGAAGGCTC TGTCTTGAAA AGGGGGCCGT ACGGCACAGA AGATGACTTT GCTGTCCCTC
1621CTTCTACCAA GCTGGCCCGG ATAGAAGAAC CAAAGAGAGT GCTGCTCTAC GTTCGAAAGG
1681AGTCAGAAGA AGTCTTTGAT GCCCTGATGC TCAAAACCCC ATCTTTGAAG GGCTTGATGG
1741AAGCTATCTC AGACAAATAC GATGTTCCCC ATGACAAGAT TGGGAAAATA TTCAAGAAGT
1801GTAAAAAGGG GATCCTGGTG AACATGGACG ACAACATTGT GAAGCATTAC TCCAATGAGG
1861ACACCTTCCA GCTGCAGATT GAAGAAGCCG GGGGGTCTTA CAAGCTCACC CTGACGGAGA
1921TCTTGCCAAC TTTCTTGTAC AAAGTTGGca ttataagaaa gcattgctta tcaatttgtt
1981gcaacgaac

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.