Transcript: Human XR_001738728.2

PREDICTED: Homo sapiens grainyhead like transcription factor 1 (GRHL1), transcript variant X9, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GRHL1 (29841)
Length:
3655
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001738728.2
NBCI Gene record:
GRHL1 (29841)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001738728.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000329982 CATCGAGGAGATTGCGTATAA pLKO_005 1106 3UTR 100% 13.200 18.480 N GRHL1 n/a
2 TRCN0000369359 TCACCCTGACGGAGATCTAAA pLKO_005 2064 3UTR 100% 13.200 10.560 N GRHL1 n/a
3 TRCN0000329981 GGACATCTGCTACGTTATTTA pLKO_005 2385 3UTR 100% 15.000 10.500 N GRHL1 n/a
4 TRCN0000369360 CTGGCTTGGTGGTAGCATTTA pLKO_005 2235 3UTR 100% 13.200 9.240 N GRHL1 n/a
5 TRCN0000017704 GCATTGACATAGCTGACTATA pLKO.1 1060 3UTR 100% 13.200 9.240 N GRHL1 n/a
6 TRCN0000017703 CCCATCTTTGAAGGGCTTGAT pLKO.1 1876 3UTR 100% 4.950 3.465 N GRHL1 n/a
7 TRCN0000329980 CCCATCTTTGAAGGGCTTGAT pLKO_005 1876 3UTR 100% 4.950 3.465 N GRHL1 n/a
8 TRCN0000017705 CCTCTTAACATTCAAGTTGAT pLKO.1 1230 3UTR 100% 4.950 3.465 N GRHL1 n/a
9 TRCN0000017706 GCCAGTGTTGGTTCTTCAGAA pLKO.1 160 3UTR 100% 4.950 3.465 N GRHL1 n/a
10 TRCN0000329979 GCCAGTGTTGGTTCTTCAGAA pLKO_005 160 3UTR 100% 4.950 3.465 N GRHL1 n/a
11 TRCN0000017707 GTGCCATTTAACATTGTCCTT pLKO.1 485 3UTR 100% 2.640 1.848 N GRHL1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001738728.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08126 pDONR223 100% 47.5% None (many diffs) n/a
2 ccsbBroad304_08126 pLX_304 0% 47.5% V5 (many diffs) n/a
3 TRCN0000478144 ACGGGGGCGGTTGAACCTTCTGTA pLX_317 12.1% 47.5% V5 (many diffs) n/a
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