Construct: ORF ccsbBroadEn_08219
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008049.1_s300c1, BRDN0000389135
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MECR (51102)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | NM_016011.5 | 99.8% | 99.7% | 286T>C;597A>G |
| 2 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | NM_001349716.2 | 92.8% | 92.7% | 177_260del;370T>C;681A>G |
| 3 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | NM_001349715.2 | 91.2% | 90.9% | 177_281del;391T>C;702A>G |
| 4 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | NM_001349717.2 | 86.4% | 84.1% | (many diffs) |
| 5 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_011541540.2 | 86.3% | 77.9% | (many diffs) |
| 6 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_011541539.1 | 81.1% | 73.2% | (many diffs) |
| 7 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | NM_001024732.4 | 79.4% | 79.3% | 0_1ins228;58T>C;369A>G |
| 8 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | NM_001349711.2 | 79.4% | 79.3% | 0_1ins228;58T>C;369A>G |
| 9 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | NM_001349712.2 | 79.4% | 79.3% | 0_1ins228;58T>C;369A>G |
| 10 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | NM_001349713.2 | 79.4% | 79.3% | 0_1ins228;58T>C;369A>G |
| 11 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | NM_001349714.2 | 79.4% | 79.3% | 0_1ins228;58T>C;369A>G |
| 12 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_017001413.1 | 79.4% | 79.3% | 0_1ins228;58T>C;369A>G |
| 13 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_024447442.1 | 79.4% | 79.3% | 0_1ins228;58T>C;369A>G |
| 14 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_024447443.1 | 79.4% | 79.3% | 0_1ins228;58T>C;369A>G |
| 15 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_024447444.1 | 79.4% | 79.3% | 0_1ins228;58T>C;369A>G |
| 16 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_024447446.1 | 79.4% | 79.3% | 0_1ins228;58T>C;369A>G |
| 17 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_024447448.1 | 79.4% | 79.3% | 0_1ins228;58T>C;369A>G |
| 18 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_024447450.1 | 79.4% | 79.3% | 0_1ins228;58T>C;369A>G |
| 19 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_011541541.1 | 79.3% | 74.7% | (many diffs) |
| 20 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XR_946663.2 | 70.9% | (many diffs) | |
| 21 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_011541543.1 | 68.7% | 60.4% | (many diffs) |
| 22 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_011541545.1 | 68.7% | 60.4% | (many diffs) |
| 23 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_011541546.1 | 68.7% | 60.4% | (many diffs) |
| 24 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_011541547.1 | 68.7% | 60.4% | (many diffs) |
| 25 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_011541549.1 | 68.7% | 60.4% | (many diffs) |
| 26 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_011541550.1 | 68.7% | 60.4% | (many diffs) |
| 27 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_011541553.1 | 68.7% | 60.4% | (many diffs) |
| 28 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_011541554.1 | 68.7% | 60.4% | (many diffs) |
| 29 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_017001411.1 | 68.7% | 60.4% | (many diffs) |
| 30 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_017001412.1 | 68.7% | 60.4% | (many diffs) |
| 31 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XR_001737209.1 | 66.4% | (many diffs) | |
| 32 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_017001416.1 | 65.2% | 60.1% | (many diffs) |
| 33 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XM_024447452.1 | 65.2% | 60.1% | (many diffs) |
| 34 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XR_002956765.1 | 61.4% | (many diffs) | |
| 35 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | XR_001737210.1 | 57.3% | (many diffs) | |
| 36 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | NR_146213.2 | 49.9% | (many diffs) | |
| 37 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | NR_146215.2 | 47.2% | (many diffs) | |
| 38 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | NR_146212.2 | 40.6% | (many diffs) | |
| 39 | human | 51102 | MECR | mitochondrial trans-2-enoyl... | NR_146214.2 | 35.3% | (many diffs) | |
| 40 | mouse | 26922 | Mecr | mitochondrial trans-2-enoyl... | XM_011250264.1 | 68.2% | 69.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1185
- ORF length:
- 1119
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGTG GGTCTGCAGT ACCCTGTGGC GGGTGCGAAC CCCCGCCCGG CAGTGGCGGG 121 GGCTGCTCCC AGCTTCTGGC TGTCACGGAC CTGCCGCCTC CTCCTACTCC GCATCCGCCG 181 AGCCTGCCCG GGTCCGGGCG CTTGTCTATG GGCACCACGG GGATCCAGCC AAGGTCGTCG 241 AACTCAAGAA CCTGGAGCTA GCTGCTGTGA GAGGATCAGA TGTCCGTGTG AAGATGCTGG 301 CGGCCCCTAT CAATCCATCT GACATAAATA TGATCCAAGG AAACTACGGA CTCCTTCCTG 361 AACTGCCTGC TGTTGGAGGG AACGAAGGTG TTGCACAGGT GGTAGCGGTG GGCAGCAATG 421 TGACCGGGCT GAAGCCAGGA GACTGGGTGA TTCCAGCAAA TGCTGGTTTA GGAACCTGGC 481 GGACCGAGGC TGTGTTCAGC GAGGAAGCAC TGATCCAAGT TCCGAGTGAC ATCCCTCTTC 541 AGAGCGCTGC CACCCTGGGT GTCAATCCCT GCACAGCCTA CAGGATGTTG ATGGACTTCG 601 AGCAACTGCA GCCAGGGGAT TCTGTCATCC AGAATGCATC CAACAGCGGA GTGGGGCAAG 661 CGGTCATCCA GATCGCCGCA GCCCTGGGCC TAAGAACCAT CAATGTGGTC CGAGACAGAC 721 CTGATATCCA GAAGCTGAGT GACAGACTGA AGAGTCTGGG GGCTGAGCAT GTCATCACAG 781 AAGAGGAGCT AAGAAGGCCC GAAATGAAAA ACTTCTTTAA GGACATGCCC CAGCCACGGC 841 TTGCTCTCAA CTGTGTTGGT GGGAAAAGCT CCACAGAGCT GCTGCGGCAG TTAGCGCGTG 901 GAGGAACCAT GGTAACCTAT GGGGGGATGG CCAAGCAGCC CGTCGTAGCC TCTGTGAGCC 961 TGCTCATTTT TAAGGATCTC AAACTTCGAG GCTTTTGGTT GTCCCAGTGG AAGAAGGATC 1021 ACAGTCCAGA CCAGTTCAAG GAGCTGATCC TCACACTGTG CGATCTCATC CGCCGAGGCC 1081 AGCTCACAGC CCCTGCCTGC TCCCAGGTCC CGCTGCAGGA CTACCAGTCT GCCTTGGAAG 1141 CCTCCATGAA GCCCTTCATA TCTTCAAAGC AGATTCTCAC CATGTGCCCA ACTTTCTTGT 1201 ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa c