Transcript: Human NM_001349716.2

Homo sapiens mitochondrial trans-2-enoyl-CoA reductase (MECR), transcript variant 8, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
MECR (51102)
Length:
2599
CDS:
29..1234

Additional Resources:

NCBI RefSeq record:
NM_001349716.2
NBCI Gene record:
MECR (51102)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001349716.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000063087 CTGGGCCTAAGAACCATCAAT pLKO.1 731 CDS 100% 5.625 7.875 N MECR n/a
2 TRCN0000063084 CCCTATCAATCCATCTGACAT pLKO.1 352 CDS 100% 4.950 6.930 N MECR n/a
3 TRCN0000063083 CTTCATATCTTCAAAGCAGAT pLKO.1 1201 CDS 100% 4.050 3.240 N MECR n/a
4 TRCN0000245051 ATCAATCCATCTGACATAAAT pLKO_005 356 CDS 100% 15.000 10.500 N MECR n/a
5 TRCN0000245054 CCTCTGTGAGCCTGCTCATTT pLKO_005 996 CDS 100% 13.200 9.240 N MECR n/a
6 TRCN0000245055 GCCAGCCTTAAGGTATCTAAT pLKO_005 1393 3UTR 100% 13.200 9.240 N MECR n/a
7 TRCN0000245052 GGTCCGAGACAGACCTGATAT pLKO_005 754 CDS 100% 13.200 9.240 N MECR n/a
8 TRCN0000245053 GAGCTAAGAAGGCCCGAAATG pLKO_005 833 CDS 100% 10.800 7.560 N MECR n/a
9 TRCN0000063085 CTCAACTGTGTTGGTGGGAAA pLKO.1 893 CDS 100% 4.050 2.430 N MECR n/a
10 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 2031 3UTR 100% 4.950 2.475 Y CFLAR n/a
11 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 2031 3UTR 100% 4.950 2.475 Y C19orf31 n/a
12 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 2029 3UTR 100% 4.950 2.475 Y ERN2 n/a
13 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 2029 3UTR 100% 4.950 2.475 Y P3H4 n/a
14 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 2029 3UTR 100% 4.950 2.475 Y P3H4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001349716.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08219 pDONR223 100% 92.8% 92.7% None 177_260del;370T>C;681A>G n/a
2 ccsbBroad304_08219 pLX_304 0% 92.8% 92.7% V5 177_260del;370T>C;681A>G n/a
3 TRCN0000470216 AGTTCCATTTCCCTCCTGACCCGT pLX_317 41.6% 92.8% 92.7% V5 177_260del;370T>C;681A>G n/a
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