Construct: ORF ccsbBroadEn_08233
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009668.1_s300c1, BRDN0000385224
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SDF4 (51150)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51150 | SDF4 | stromal cell derived factor 4 | NM_016176.4 | 99.9% | 100% | 570T>C |
| 2 | human | 51150 | SDF4 | stromal cell derived factor 4 | NM_016547.2 | 88.9% | 82.8% | (many diffs) |
| 3 | human | 51150 | SDF4 | stromal cell derived factor 4 | XM_024447473.1 | 88.8% | 88.9% | 570T>C;736_870del |
| 4 | human | 51150 | SDF4 | stromal cell derived factor 4 | XM_011541556.1 | 55% | 53.8% | (many diffs) |
| 5 | mouse | 20318 | Sdf4 | stromal cell derived factor 4 | NM_001302467.1 | 82.8% | 87% | (many diffs) |
| 6 | mouse | 20318 | Sdf4 | stromal cell derived factor 4 | NM_001302468.1 | 82.8% | 87% | (many diffs) |
| 7 | mouse | 20318 | Sdf4 | stromal cell derived factor 4 | NM_011341.5 | 82.8% | 87% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1152
- ORF length:
- 1086
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGT CTGGCCCTGG GTGGCGATGG CGTCCAGGTG GGGTCCCCTC ATTGGCCTGG 121 CTCCGTGCTG CCTCTGGCTC CTGGGGGCAG TCCTTCTGAT GGACGCGTCT GCACGGCCTG 181 CCAACCACTC GTCCACTCGA GAGAGAGTAG CCAACAGGGA GGAGAATGAG ATCCTGCCCC 241 CAGACCACCT GAACGGGGTG AAGCTGGAGA TGGACGGGCA CCTCAATCGC GGCTTCCACC 301 AGGAGGTCTT CCTAGGCAAG GACCTGGGTG GCTTTGATGA GGACGCGGAG CCGCGGCGGA 361 GCCGGAGGAA GCTGATGGTC ATCTTTTCCA AGGTGGATGT GAACACTGAC CGGAAGATCA 421 GTGCCAAGGA GATGCAGCGC TGGATCATGG AGAAGACGGC CGAGCACTTC CAGGAGGCCA 481 TGGAGGAGAG CAAGACACAC TTCCGCGCCG TGGACCCTGA CGGGGACGGT CACGTGTCTT 541 GGGACGAGTA TAAGGTGAAG TTTTTGGCGA GTAAAGGCCA TAGCGAGAAG GAGGTTGCCG 601 ACGCCATCAG GCTCAACGAG GAACTCAAAG TGGACGAGGA AACACAGGAA GTCCTGGAGA 661 ACCTGAAGGA CCGCTGGTAC CAGGCGGACA GCCCCCCTGC AGACCTGCTG CTGACGGAGG 721 AGGAGTTCCT GTCGTTCCTC CACCCCGAGC ACAGCCGGGG AATGCTCAGG TTCATGGTGA 781 AGGAGATCGT CCGGGACCTG GACCAGGACG GTGACAAGCA GCTCTCTGTG CCCGAGTTCA 841 TCTCCCTGCC CGTGGGCACC GTGGAGAACC AGCAGGGCCA GGACATTGAC GACAACTGGG 901 TGAAAGACAG AAAAAAGGAG TTTGAGGAGC TCATTGACTC CAACCACGAC GGCATCGTGA 961 CCGCCGAGGA GCTGGAGAGC TACATGGACC CCATGAACGA GTACAACGCG CTGAACGAGG 1021 CCAAGCAGAT GATCGCCGTC GCCGACGAGA ACCAGAACCA CCACCTGGAG CCCGAGGAGG 1081 TGCTCAAGTA CAGCGAGTTC TTCACGGGCA GCAAGCTGGT GGACTACGCG CGCAGCGTGC 1141 ACGAGGAGTT TTGCCCAACT TTCTTGTACA AAGTtggcat tataagaaag cattgcttat 1201 caatttgttg caacgaac