Construct: ORF ccsbBroadEn_08238

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013472.1_s300c1, BRDN0000389617
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DCTN4 (51164)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51164 DCTN4 dynactin subunit 4 NM_016221.3 99.7% 99.3% (many diffs)
2 human 51164 DCTN4 dynactin subunit 4 NM_001135643.1 98.2% 97.8% (many diffs)
3 human 51164 DCTN4 dynactin subunit 4 NM_001135644.1 87.3% 87.1% (many diffs)
4 human 51164 DCTN4 dynactin subunit 4 XM_011537644.1 87.3% 87.1% (many diffs)
5 human 51164 DCTN4 dynactin subunit 4 XM_011537645.1 87.3% 87.1% (many diffs)
6 human 51164 DCTN4 dynactin subunit 4 XM_011537646.1 82.6% 82.3% (many diffs)
7 human 51164 DCTN4 dynactin subunit 4 XM_005268457.1 68.7% 68.6% 0_1ins429;594C>A;948T>N
8 human 51164 DCTN4 dynactin subunit 4 XM_011537647.1 68.7% 68.6% 0_1ins429;594C>A;948T>N
9 mouse 67665 Dctn4 dynactin 4 NM_026302.3 89.4% 96% (many diffs)
10 mouse 67665 Dctn4 dynactin 4 XM_006526189.3 88% 94.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1446
ORF length:
1380
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC GTCCTTGCTG CAGTCGGACC GGGTTCTCTA TCTAGTCCAG GGAGAAAAGA
121AGGTTCGGGC CCCGCTCTCG CAACTCTACT TCTGCCGCTA TTGTAGCGAA CTGCGGTCGC
181TGGAATGTGT GTCTCACGAG GTGGNCTCCC ATTATTGTCC CAGTTGTTTA GAAAATATGC
241CATCGGCTGA AGCCAAACTA AAAAAGAATA GATGTGCCAA TTGTTTTGAC TGTCCTGGCT
301GCATGCACAC CCTCTCTACT CGGGCCACGA GCATCTCCAC ACAGCTTACA GATGACCCAG
361CCAAGACCAC CATGAAGAAA GCCTATTACC TGGCATGTGG ATTTTGTCGC TGGACGTCTA
421GAGATGTGGG CATGGCAGAC AAATCTGTAG CTAGTGGCGG TTGGCAGGAA CCTGAAAATC
481CTCACACACA ACGGATGAAC AAATTGATTG AATATTACCA GCAGCTTGCT CAGAAAGAGA
541AGGTTGAGCG AGATCGCAAG AAACTGGCAC GACGTAGAAA CTATATGCCT CTGGCTTTTT
601CGGACAAATA TGGTCTTGGA ACCAGGCTTC AGCGACCACG AGCTGGTGCA TCCATCAGTA
661CCCTTGCCGG ACTTTCCCTT AAAGAAGGAG AGGATCAGAA AGAGATAAAG ATTGAGCCAG
721CTCAGGCTGT GGATGAAGTG GAACCTCTAC CTGAAGACTA TTATACAAGA CCAGTAAATT
781TAACAGAGGT AACAACCCTT CAGCAGCGTC TGTTACAGCC TGACTTCCAG CCAGTCTGTG
841CTTCACAGCT CTATCCTCGC CACAAACATC TTCTGATCAA ACGGTCCCTG CGCTGCCGTA
901AATGTGAACA TAATTTGAGC AAGCCAGAAT TTAACCCAAC GTCAATCAAA TTCAAAATCC
961AGCTGGTCGC TGTCAATTAT ATTCCAGAAG TGAGAATCAT GTCAATTCCC AACCTTCGCT
1021ACATGAAGGA GAGCCAGGTC CTCCTGACTC TTACAAATCC AGTTGAGAAC CTCACCCATG
1081TGACTCTATT CGAGTGTGAG GAGGGGGACC CTGATGATAT CAACAGCACT GCTAAGGTGG
1141TGGTGCCTCC CAAAGAGCTC GTTTTAGCTG GCAAGGATGC AGCAGCAGAG TACGATGAGT
1201TGGCAGAACC TCAAGACTTT CAGGACGATC CTGACATTAT AGCCTTCAGA AAGGCCAACA
1261AAGTGGGTAT TTTCATCAAA GTTACACCAC AGCGTGAGGA GGGTGAAGTG ACCGTGTGCT
1321TCAAGATGAA GCATGATTTT AAAAACCTGG CAGCCCCCAT TCGCCCCATT GAAGAAAGTG
1381ACCAGGGAAC AGAAGTCATC TGGCTCACCC AGCATGTGGA ACTTAGCTTG GGCCCACTTC
1441TNCCTTACCC AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc ttatcaattt
1501gttgcaacga ac

Download FASTA (ORF) (Full)