Construct: ORF ccsbBroadEn_08329
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004251.1_s300c1, BRDN0000394361
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCZ1 (51622)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51622 | CCZ1 | CCZ1 homolog, vacuolar prot... | NM_015622.6 | 99.8% | 99.7% | 759T>C;1421A>C |
| 2 | human | 221960 | CCZ1B | CCZ1 homolog B, vacuolar pr... | NM_198097.3 | 99.8% | 99.7% | 1104T>C;1421A>C |
| 3 | mouse | 231874 | Ccz1 | CCZ1 vacuolar protein traff... | NM_177682.3 | 88.4% | 95.6% | (many diffs) |
| 4 | mouse | 231874 | Ccz1 | CCZ1 vacuolar protein traff... | XM_017320876.1 | 62.2% | 67.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1515
- ORF length:
- 1446
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGCTGCAGCG GCGGCCGGGG CCGGGAGCGG GCCCTGGGCG GCCCAGGAGA 121 AGCAGTTCCC GCCGGCGCTG CTGAGTTTCT TCATCTACAA CCCGCGCTTC GGGCCGCGCG 181 AAGGACAGGA GGAAAATAAG ATTTTATTTT ATCATCCAAA TGAGGTAGAA AAGAATGAGA 241 AGATTAGAAA TGTCGGATTG TGTGAAGCTA TTGTACAGTT TACAAGGACA TTTAGCCCAT 301 CAAAACCTGC AAAATCTTTA CATACACAGA AGAACAGACA GTTCTTCAAT GAACCAGAAG 361 AAAATTTCTG GATGGTCATG GTTGTTCGGA ATCCTATAAT TGAAAAACAG AGTAAAGATG 421 GAAAACCAGT TATTGAATAT CAAGAGGAGG AGTTGTTGGA CAAGGTTTAT AGCTCGGTGC 481 TGCGGCAGTG CTACAGCATG TACAAGCTTT TTAATGGTAC ATTTCTGAAA GCCATGGAAG 541 ACGGAGGCGT CAAGCTTCTG AAAGAAAGAT TAGAGAAATT CTTCCATCGG TATTTGCAAA 601 CGCTACATTT GCAGTCATGT GACCTACTTG ACATTTTTGG TGGAATCAGC TTCTTCCCGT 661 TGGATAAAAT GACTTATTTG AAAATCCAGT CCTTTATTAA TAGAATGGAG GAAAGCCTGA 721 ATATAGTCAA ATACACTGCT TTTCTCTATA ACGATCAGCT CATCTGGAGT GGATTAGAAC 781 AAGATGACAT GAGAATTTTA TACAAATACC TTACCACCTC CCTTTTCCCA AGGCACATCG 841 AACCTGAGTT AGCAGGAAGG GATTCTCCAA TAAGAGCAGA AATGCCAGGA AATCTTCAAC 901 ACTATGGAAG ATTTCTTACC GGACCCTTGA ACCTCAATGA TCCAGATGCA AAATGCAGAT 961 TCCCCAAAAT TTTTGTAAAT ACAGATGACA CTTATGAAGA GCTCCATTTA ATCGTTTATA 1021 AGGCCATGAG TGCGGCTGTG TGCTTTATGA TCGACGCCTC TGTCCACCCA ACGTTGGATT 1081 TTTGCCGAAG ACTGGACAGC ATCGTTGGGC CCCAGCTCAC AGTGCTGGCC TCTGACATCT 1141 GTGAACAGTT TAACATCAAC AAGAGGATGT CcgggTCTGA GAAAGAACCC CAGTTTAAGT 1201 TTATCTACTT CAACCACATG AATCTCGCCG AGAAGAGCAC AGTTCACATG AGGAAAACGC 1261 CCAGCGTGTC GCTCACTTCC GTGCACCCGG ATTTAATGAA GATTCTCGGT GACATCAACA 1321 GTGACTTTAC CAGAGTGGAT GAAGATGAGG AGATCATTGT GAAGGCCATG AGTGATTACT 1381 GGGTTGTTGG AAAGAAGTCT GATCGGCGGG AGCTCTATGT TATTTTGAAT CAAAAAAATG 1441 CAAACCTGAT TGAAGTAAAT GAAGAGGTCA AGAAACTTTG TGCAACGCCG TTCAACAACA 1501 TCTTCTTCTT GGATTTGCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct 1561 tatcaatttg ttgcaacgaa c