Construct: ORF ccsbBroadEn_08383

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008641.1_s300c1, BRDN0000396549
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NLE1 (54475)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54475 NLE1 notchless homolog 1 NM_018096.5 99.7% 99.3% 16C>G;506G>A;955C>A
2 human 54475 NLE1 notchless homolog 1 NM_001014445.2 39.7% 39.5% 0_1ins876;79C>A
3 human 54475 NLE1 notchless homolog 1 XM_017024777.1 39.7% 39.5% 0_1ins876;79C>A
4 mouse 217011 Nle1 notchless homolog 1 (Drosop... NM_145431.2 89.2% 95.2% (many diffs)
5 mouse 217011 Nle1 notchless homolog 1 (Drosop... XR_879591.1 70% (many diffs)
6 mouse 217011 Nle1 notchless homolog 1 (Drosop... XR_388409.2 69.9% (many diffs)
7 mouse 217011 Nle1 notchless homolog 1 (Drosop... XM_006532975.2 58.4% 62.2% (many diffs)
8 mouse 217011 Nle1 notchless homolog 1 (Drosop... XM_006532976.2 51.3% 54% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1521
ORF length:
1455
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC GGCAGCAGTG GCGGACGAGG CGGTGGCGCG CGATGTGCAG CGGTTGCTAG
121TGCAGTTCCA GGATGAGGGC GGGCAGCTGC TGGGTTCCCC GTTCGACGTG CCCGTGGACA
181TCACCCCGGA CAGGCTGCAG CTCGTGTGCA ACGCGCTACT GGCCCAGGAG GATCCCCTGC
241CACTGGCTTT CTTTGTCCAC GATGCTGAGA TCGTCTCCTC ACTGGGGAAG ACGTTGGAGT
301CCCAGGCAGT GGAGACAGAG AAGGTCCTAG ACATCATCTA CCAGCCACAG GCTATCTTCA
361GAGTCCGGGC TGTGACTCGC TGCACCAGCT CCTTGGAGGG TCACAGTGAG GCAGTCATTT
421CTGTGGCCTT CAGCCCTACG GGAAAGTACC TGGCCAGTGG CTCTGGAGAC ACCACCGTGC
481GCTTCTGGGA TCTCAGCACA GAGACACCAC ATTTCACATG CAAGGGACAC AGACACTGGG
541TCCTTAGTAT ATCCTGGTCT CCAGATGGCA AGAAGCTGGC CTCAGGCTGC AAGAATGGCC
601AGATTCTCCT CTGGGACCCA AGCACAGGGA AGCAGGTGGG CAGGACCCTC GCTGGCCACA
661GCAAGTGGAT CACAGGCCTG AGCTGGGAGC CCCTCCATGC GAACCCTGAG TGCCGCTATG
721TGGCCAGCAG CTCCAAGGAT GGCAGTGTGC GGATCTGGGA CACAACTGCA GGCCGCTGTG
781AGCGCATCCT CACCGGGCAC ACCCAGTCGG TCACCTGTCT CCGGTGGGGA GGGGACGGGC
841TTCTCTACTC TGCCTCCCAG GACCGCACCA TCAAAGTCTG GAGAGCTCAT GACGGTGTGC
901TGTGCCGGAC TCTGCAAGGC CACGGCCACT GGGTGAACAC CATGGCCCTC AGCACTGACT
961ATGCCCTGCG CACTGGGGCC TTTGAACCTG CTGAGGCCTC AGTTAATCCC CAAGACCTCA
1021AAGGATCCTT GCAGGAGTTG AAGGAGAGGG CTCTGAGCCG ATACAACCTC GTGCGGGGCC
1081AGGGTCCAGA GAGGCTGGTG TCTGGCTCCG ACGACTTCAC CTTATTCCTG TGGTCCCCAG
1141CAGAGGACAA AAAGCCTCTC ACTCGGATGA CAGGACACCA AGCTCTCATC AACCAGGTGC
1201TCTTCTCTCC TGACTCCCGC ATCGTGGCTA GTGCCTCCTT TGACAAGTCC ATCAAGCTGT
1261GGGATGGCAG GACGGGCAAG TACCTGGCTT CCCTACGCGG CCACGTGGCT GCCGTGTACC
1321AGATTGCGTG GTCAGCTGAC AGTCGGCTCC TGGTCAGCGG CAGCAGTGAC AGCACACTGA
1381AGGTGTGGGA TGTGAAGGCC CAGAAGCTGG CCATGGACCT GCCCGGCCAC GCGGATGAGG
1441TATATGCTGT TGACTGGAGT CCAGATGGCC AGAGAGTGGC AAGTGGTGGG AAGGACAAAT
1501GCCTCCGGAT ATGGAGGAGA TGCCCAACTT TCTTGTACAA AGTtggcatt ataagaaagc
1561attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)