Transcript: Human XM_017024777.1

PREDICTED: Homo sapiens notchless homolog 1 (NLE1), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NLE1 (54475)
Length:
5198
CDS:
935..1516

Additional Resources:

NCBI RefSeq record:
XM_017024777.1
NBCI Gene record:
NLE1 (54475)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017024777.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000275230 CTCCGACGACTTCACCTTATT pLKO_005 1099 CDS 100% 13.200 18.480 N NLE1 n/a
2 TRCN0000275229 GACACTGGGTCCTTAGTATAT pLKO_005 525 5UTR 100% 13.200 18.480 N NLE1 n/a
3 TRCN0000155612 CATCAAAGTCTGGAGAGCTCA pLKO.1 862 5UTR 100% 2.640 2.112 N NLE1 n/a
4 TRCN0000275228 CCAGGTTCTATGACCAAATAA pLKO_005 1855 3UTR 100% 15.000 10.500 N NLE1 n/a
5 TRCN0000154710 GACAGAGAAGGTCCTAGACAT pLKO.1 278 5UTR 100% 4.950 3.465 N NLE1 n/a
6 TRCN0000275231 GACAGAGAAGGTCCTAGACAT pLKO_005 278 5UTR 100% 4.950 3.465 N NLE1 n/a
7 TRCN0000179337 GACACCACATTTCACATGCAA pLKO.1 496 5UTR 100% 3.000 2.100 N NLE1 n/a
8 TRCN0000275232 GACACCACATTTCACATGCAA pLKO_005 496 5UTR 100% 3.000 2.100 N NLE1 n/a
9 TRCN0000195929 CTAGACATCATCTACCAGCCA pLKO.1 291 5UTR 100% 0.660 0.462 N NLE1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017024777.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08383 pDONR223 100% 39.7% 39.5% None 0_1ins876;79C>A n/a
2 ccsbBroad304_08383 pLX_304 0% 39.7% 39.5% V5 0_1ins876;79C>A n/a
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