Construct: ORF ccsbBroadEn_08417

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001527.1_s300c1, BRDN0000394951
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ESRP1 (54845)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54845 ESRP1 epithelial splicing regulat... NM_017697.4 99.9% 100% 984C>T;1800T>C
2 human 54845 ESRP1 epithelial splicing regulat... NM_001034915.3 99.3% 99.4% 984C>T;1635_1636insCCATGTAAGTTA;1788T>C
3 human 54845 ESRP1 epithelial splicing regulat... XM_005250991.3 96.8% 96.4% (many diffs)
4 human 54845 ESRP1 epithelial splicing regulat... NM_001122826.2 96.2% 95.8% (many diffs)
5 human 54845 ESRP1 epithelial splicing regulat... XM_005250992.3 91.7% 90.1% (many diffs)
6 human 54845 ESRP1 epithelial splicing regulat... NM_001122825.2 89.1% 89.1% (many diffs)
7 human 54845 ESRP1 epithelial splicing regulat... NM_001122827.2 88.5% 88.5% (many diffs)
8 mouse 207920 Esrp1 epithelial splicing regulat... NM_194055.3 90.7% 97.2% (many diffs)
9 mouse 207920 Esrp1 epithelial splicing regulat... NM_001290383.1 90.6% 97% (many diffs)
10 mouse 207920 Esrp1 epithelial splicing regulat... XM_006537715.1 90.1% 96.6% (many diffs)
11 mouse 207920 Esrp1 epithelial splicing regulat... XM_006537716.1 87.7% 93.8% (many diffs)
12 mouse 207920 Esrp1 epithelial splicing regulat... XM_006537717.1 82.9% 87.6% (many diffs)
13 mouse 207920 Esrp1 epithelial splicing regulat... XM_006537718.1 80.2% 86.6% (many diffs)
14 mouse 207920 Esrp1 epithelial splicing regulat... NM_001347052.1 79.6% 86% (many diffs)
15 mouse 207920 Esrp1 epithelial splicing regulat... XM_006537721.3 65.2% 69.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2112
ORF length:
2043
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GACGGCCTCT CCGGATTACT TGGTGGTGCT TTTTGGGATC ACTGCTGGGG
121CCACCGGGGC CAAGCTAGGC TCGGATGAGA AGGAGTTGAT CCTGCTGTTC TGGAAAGTCG
181TGGATCTGGC CAACAAGAAG GTGGGACAGT TGCACGAAGT GCTAGTTAGA CCGGATCAGT
241TGGAACTGAC GGAGGACTGC AAAGAAGAAA CTAAAATAGA CGTCGAAAGC CTGTCCTCGG
301CGTCGCAGCT GGACCAAGCC CTCCGACAGT TTAACCAGTC AGTGAGCAAT GAACTGAATA
361TTGGAGTAGG GACTTCCTTC TGTCTCTGTA CTGATGGGCA GCTTCATGTC AGGCAAATCC
421TGCATCCTGA GGCTTCCAAG AAGAATGTAC TATTACCTGA ATGCTTCTAT TCCTTTTTTG
481ATCTTCGAAA AGAATTCAAG AAATGTTGCC CTGGTTCACC TGATATTGAC AAACTGGACG
541TTGCCACAAT GACAGAGTAT TTAAATTTTG AGAAGAGTAG TTCAGTCTCT CGATATGGAG
601CCTCTCAAGT TGAAGATATG GGGAATATAA TTTTAGCAAT GATTTCAGAG CCTTATAATC
661ACAGGTTTTC AGATCCAGAG AGAGTGAATT ACAAGTTTGA AAGTGGAACT TGCAGCAAGA
721TGGAACTTAT TGATGATAAC ACCGTAGTCA GGGCACGAGG TTTACCATGG CAGTCTTCAG
781ATCAAGATAT TGCAAGATTC TTCAAAGGAC TCAATATTGC CAAGGGAGGT GCAGCACTTT
841GTCTGAATGC TCAGGGTCGA AGGAACGGAG AAGCTCTGGT TAGGTTTGTA AGTGAGGAGC
901ACCGAGACCT AGCACTACAG AGGCACAAAC ATCACATGGG GACCCGGTAT ATTGAGGTTT
961ACAAAGCAAC AGGTGAAGAT TTCCTTAAAA TTGCTGGTGG TACTTCCAAT GAGGTAGCCC
1021AGTTTCTCTC CAAGGAAAAT CAAGTCATTG TTCGCATGCG GGGGCTCCCT TTCACGGCCA
1081CAGCTGAAGA AGTGGTGGCC TTCTTTGGAC AGCATTGCCC TATTACTGGG GGAAAGGAAG
1141GCATCCTCTT TGTCACCTAC CCAGATGGTA GGCCAACAGG GGACGCTTTT GTCCTCTTTG
1201CCTGTGAGGA ATATGCACAG AATGCGTTGA GGAAGCATAA AGACTTGTTG GGTAAAAGAT
1261ACATTGAACT CTTCAGGAGC ACAGCAGCTG AAGTTCAGCA GGTGCTGAAT CGATTCTCCT
1321CGGCCCCTCT CATTCCACTT CCAACCCCTC CCATTATTCC AGTACTACCT CAGCAATTTG
1381TGCCCCCTAC AAATGTTAGA GACTGTATAC GCCTTCGAGG TCTTCCCTAT GCAGCCACAA
1441TTGAGGACAT CCTGGATTTC CTGGGGGAGT TCGCCACAGA TATTCGTACT CATGGGGTTC
1501ACATGGTTTT GAATCACCAG GGCCGCCCAT CAGGAGATGC CTTTATCCAG ATGAAGTCTG
1561CGGACAGAGC ATTTATGGCT GCACAGAAGT GTCATAAAAA AAACATGAAG GACAGATATG
1621TTGAAGTCTT TCAGTGTTCA GCTGAGGAGA TGAACTTTGT GTTAATGGGG GGCACTTTAA
1681ATCGAAATGG CTTATCCCCA CCGCCATGTA AGTTACCATG CCTGTCTCCT CCCTCCTACA
1741CATTTCCAGC TCCTGCTGCA GTTATTCCTA CAGAAGCTGC CATTTACCAG CCCTCTGTGA
1801TTTTGAATCC ACGAGCACTG CAGCCCTCCA CAGCGTACTA CCCAGCAGGC ACTCAGCTCT
1861TCATGAACTA CACAGCGTAC TATCCCAGCC CCCCAGGTTC GCCTAATAGT CTTGGCTACT
1921TCCCTACAGC TGCTAATCTT AGCGGTGTCC CTCCACAGCC TGGCACGGTG GTCAGAATGC
1981AGGGCCTGGC CTACAATACT GGAGTTAAGG AAATTCTTAA CTTCTTCCAA GGTTACCAGT
2041ATGCAACCGA GGATGGACTT ATACACACAA ATGACCAGGC CAGGACTCTA CCCAAAGAAT
2101GGGTTTGTAT TTTGCCAACT TTCTTGTACA AAGTtggcat tataagaaag cattgcttat
2161caatttgttg caacgaac

Download FASTA (ORF) (Full)